Palabras clave
McCoy cell line
Rabies Virus
(CVS, ERA, PVl and street virus).Isolation
titration and cytopathic effect (CPE)
Rabies Virus
(CVS, ERA, PVl and street virus).Isolation
titration and cytopathic effect (CPE)
Cómo citar
1.
Nogueira YL, Amaral CFC do. Rabies virus in McCoy cell line: Part II – Titration. Rev Inst Adolfo Lutz [Internet]. 30 de diciembre de 1992 [citado 18 de mayo de 2024];52(1-2):17-21. Disponible en: https://periodicos.saude.sp.gov.br/RIAL/article/view/35959
Resumen
The McCoy cell line has showed high sensivity to rabies virus. This cell line presents cythopatic effect (CPE), when infected with rabies virus. Three strains of rabies virus were tested: CVS, ERA, and PV1 and three street virus were isolated: one from a patient bitten by bat, other from a rabid dog and another from bovine. This cell line was used for virus titration purposes due to the presence of cytophatic effect, two techniques for observation of the CPE were used and were compared to the classical method of intracerebral inoculation in mice.
Citas
1. BLANCOU, J.; AUBERT, M.F.A. & CAIN, E. - Comparaison de Quatre Techniques de Titrages des Anticorps Contre le Vírus de Ia Rage Chez le Chien. J. Biol. Standart.11: 271 - 277,1983.
2. HABEL, K. - Habel test for potency. In: KAPLAN, M. & KOPROWSKI, H., ed. Laboratory techniques in rabies, 3rd ed. Geneva, WHO, 1973. P. 290. (Monograph Series).
3. HEBERLING, R. L.; KALTER, S. S.; SMITH, J. S. & HILDEBRAND, D. G. - Serodiagnosis of
rabies by dot immunobinding assay. J. clin. Microbiol. July 1262 - 1264, 1987.
4. MANNEN, K.; MIFUNE, K.; SNADEN, R.; SMITH, J. S.; YAGER, P. A., SUMNER, J. w.; FISHBEIN, D. B.; TONG, T. C. & BAER, G. M. - Microneutralization test for rabies virus vased on an enzyme immunoassay. J. clin. Microbiol. Dec. 2440 - 2443, 1987.
5. NOGUEIRA, Y. L. - Cytopathie effect on tissue culture caused by rabies virus. In: INTERNATIONAL CONFERENCE ON IMPACT OF VIRAL DISEASES ON DEVELOPMENT OF LATIN AMERICAN COUNTRIES AND CARIBBEAN REGION, 1st, Rio de Janeiro, Brazil, 1982. Abstracts. Rio de Janeiro, Oswaldo Cruz Foundation, 1982, p. 113.
6. NOGUEIRA, Y. L. - Rabies virus in McCoy eellline. Part I: Cytopathie effect and replication. Rev. Inst. Adolfo Lutz, 1992. (ln press).
7. PERRIN, P.; ROLLIN, P. E. & SUREAU, P. - A Rapid enzyme immunodiagnosis (RREID): A usefulk and simple technique for the routine diagnosis of rabies. J. biol. Standart.14:: 217-222,1986.
8. RUDD, R. 1. & TRIMARCHI, C. V. – Comparation of sensitivity of BHK-21 and murine
neuroblastoma cells line in the isolation of a street strain rabies virus. J. clin. Microblol. Aug. 1456 - 1458, 1987.
9. REED-MUENCH - methods for calculation of end point. In KAPLAN, M. & KOPROWSKI, H. - Laboratory Techniques in Rabies, 3rd ed., Geneve, WHO, 1973, p. 348 - 3544 (Monograph Series).
10. SMITH, A. L.; TIGNOR, G. H.; EMMONS, R. W. & WOODIE, J. D. - Isolation of field rabies virus strain in CER and murine neuroblastoma cell cultures. Intervirology 9: 359-361, 1978.
11. WEBSTER, W. A. - A tissue culture infection test in routine rabies diagnosis. Can. J. Vet. Rev. 51: 367 - 369,1987.
12. WIKTOR, T. J. & CLARK, H. F. - Chronic rabies infection of cell cultures. Infect. Immun. Dec.: 988 - 995, 1972.
2. HABEL, K. - Habel test for potency. In: KAPLAN, M. & KOPROWSKI, H., ed. Laboratory techniques in rabies, 3rd ed. Geneva, WHO, 1973. P. 290. (Monograph Series).
3. HEBERLING, R. L.; KALTER, S. S.; SMITH, J. S. & HILDEBRAND, D. G. - Serodiagnosis of
rabies by dot immunobinding assay. J. clin. Microbiol. July 1262 - 1264, 1987.
4. MANNEN, K.; MIFUNE, K.; SNADEN, R.; SMITH, J. S.; YAGER, P. A., SUMNER, J. w.; FISHBEIN, D. B.; TONG, T. C. & BAER, G. M. - Microneutralization test for rabies virus vased on an enzyme immunoassay. J. clin. Microbiol. Dec. 2440 - 2443, 1987.
5. NOGUEIRA, Y. L. - Cytopathie effect on tissue culture caused by rabies virus. In: INTERNATIONAL CONFERENCE ON IMPACT OF VIRAL DISEASES ON DEVELOPMENT OF LATIN AMERICAN COUNTRIES AND CARIBBEAN REGION, 1st, Rio de Janeiro, Brazil, 1982. Abstracts. Rio de Janeiro, Oswaldo Cruz Foundation, 1982, p. 113.
6. NOGUEIRA, Y. L. - Rabies virus in McCoy eellline. Part I: Cytopathie effect and replication. Rev. Inst. Adolfo Lutz, 1992. (ln press).
7. PERRIN, P.; ROLLIN, P. E. & SUREAU, P. - A Rapid enzyme immunodiagnosis (RREID): A usefulk and simple technique for the routine diagnosis of rabies. J. biol. Standart.14:: 217-222,1986.
8. RUDD, R. 1. & TRIMARCHI, C. V. – Comparation of sensitivity of BHK-21 and murine
neuroblastoma cells line in the isolation of a street strain rabies virus. J. clin. Microblol. Aug. 1456 - 1458, 1987.
9. REED-MUENCH - methods for calculation of end point. In KAPLAN, M. & KOPROWSKI, H. - Laboratory Techniques in Rabies, 3rd ed., Geneve, WHO, 1973, p. 348 - 3544 (Monograph Series).
10. SMITH, A. L.; TIGNOR, G. H.; EMMONS, R. W. & WOODIE, J. D. - Isolation of field rabies virus strain in CER and murine neuroblastoma cell cultures. Intervirology 9: 359-361, 1978.
11. WEBSTER, W. A. - A tissue culture infection test in routine rabies diagnosis. Can. J. Vet. Rev. 51: 367 - 369,1987.
12. WIKTOR, T. J. & CLARK, H. F. - Chronic rabies infection of cell cultures. Infect. Immun. Dec.: 988 - 995, 1972.
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Derechos de autor 1992 Revista del Instituto Adolfo Lutz
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