Keywords
ante-mortem diagnosis
cerebrospinal fluid
virus neutralizing antibodies
How to Cite
Abstract
The laboratorial ante-mortem diagnosis of human rabies include the detection of rabies virus in skin biopsy, saliva, cerebrospinal fluid (CSF), corneal smear and also the detection of rabies virus-neutralizing antibodies (VNA) in serum or CSF samples. The detection of rabies VNA in the serum or CSF of unvaccinated individuals is diagnostic of rabies, but it occurs late in course of the disease. In this study were analyzed the results of VNA in serum and /or CSF of three cases of patients suspect of rabies, with no history of previous vaccination or passive immunization. The VNA evaluation was performed by the simplified fluorescence inhibition microtest. The patient A received diagnostic of rabies, despite of there wasn’t analyses of central nervous system (CNS), supported by the clinical symptoms and results of VNA titers of 3,0 IU/mL in serum and 0,37 IU/mL in CSF. About the two patients who had the rabies virus identified for the techniques post-mortem in the CNS, the patient B presented 12 IU/mL of VNA in CSF and the patient C had negative results of VNA titers in serum and CSF samples, which were compatible to period of morbidity. These results indicated that the determination of VNA in serum and CSF samples of rabies human suspect cases, with no history of previous vaccination or passive immunization should contribute to ante-mortem diagnosis if successive collects were done to evaluate the progression of VNA titers establishing more safety in diagnostic, especially in those cases that the brain tissue can be collected for any reason.
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Copyright (c) 2007 Luciana Botelho Chaves, Andréa de Cássia Rodrigues Silva, Graciane Maria Medeiros Caporale, Karin Corrêa Scheffer, Salim Jorge Waquim Neto, Maria Luiza Carrieri, Ivanete Kotait