Keywords
Dot-ELISA
capture ELISA
agglutination with latex particles
food samples
How to Cite
Abstract
This paper describes the production and the use of new monoclonal antibodies for the detection of the bacteria Vibrio cholerae , Escherichia coli O157:H7 as well as for the toxins Stx1 e Stx2 in food samples. Immunological tests like Dot-ELISA - Capture and agglutination ELISA, employing latex particles liked to the monoclonal antibodies produced are under evaluation. Limits and microbiological patterns that must be adopted to warrant correct interpretation of the results of microbiological results, factors contributing to the occurrence of foodborne diseases outbreaks demand the implantation of detection systems of high specificity and sensibility, which are characteristic of these immunological techniques. Data indicate that Dot-ELISA, Capture ELISA Agglutination essays and latex particles are appropriate, depending on the food sample under analysis.
References
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This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 2007 Ruth E G Rowlands, Christiane A Ristori, Tatiane Ferreira, André Yoshio Yto, Daniela de Lima Franco, Monica C G Scola, Mioko Jakabi, Dilma S Gelli, Mark Tamplin, Tulio N. Cunha, Elizabeth N. De Gaspari