Palavras-chave
protozoários ciliados
diversidade biológica
vegetais folhosos
contaminação alimentar
doenças transmitidas por alimentos
segurança alimentar
diversidade biológica
vegetais folhosos
contaminação alimentar
doenças transmitidas por alimentos
segurança alimentar
Como Citar
1.
Bonatti TR, Yamashiro S, Freitas PF de CS e, Siqueira-Castro ICV, Franco RMB. Ocorrência e diversidade de protozoários ciliados nos vegetais folhosos mais consumidos no Brasil: um estudo qualitativo. Rev Inst Adolfo Lutz [Internet]. 20º de dezembro de 2020 [citado 5º de novembro de 2024];79(1):1-9. Disponível em: https://periodicos.saude.sp.gov.br/RIAL/article/view/36027
Resumo
Os protozoários ciliados constituem um grupo onipresente de eucariotos pouco relatados em vegetais
folhosos. O presente estudo teve como objetivo verificar a ocorrência e a diversidade de protozoários
ciliados nas folhas e raízes de três dos vegetais folhosos mais consumidos no Brasil - alface, rúcula
e coentro. As amostras de vegetais foram lavadas por agitação manual (3 minutos) em dois meios
diferentes (água mineral e Solução Salina para Ameba). Após a lavagem, o conteúdo foi incubado
em placas de Petri e alíquotas foram retiradas para identificação microscópica e observação in vivo
dos organismos. Um total de 21 espécies de protozoários ciliados foi encontrado, a maioria das quais
era bacterívora. Tais hortaliças têm sido comumente associadas a surtos transmitidos por alimentos
e os protozoários ciliados que, embora não sejam um problema de saúde pública, podem atuar como
“Cavalos de Tróia” ao abrigar bactérias, vírus além de cistos e oocistos de protozoários o que pode
sugerir uma nova rota para a avaliação da qualidade microbiológica relacionada à cadeia alimentar.
Este é o primeiro relato de protozoários ciliados em folhas verdes consumidas no Brasil.
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fm.2015.11.013
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org//10.1128/AEM.02709-07
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and transmission of pathogenic bacteria. Front
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fmicb.2014.00240
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environmental waters. Cooperative Research Centre
for Water Quality and Treatment, Salisbury. Research
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https://www.waterra.com.au/publications/documentsearch/?
download=81
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1988; 54(12): 3023-33. https://dx.doi.org/ 10.1128/
AEM.54.12.3023-3033.1988
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on common sold vegetables in Alqalamoun region. Int
J Pharm Sci Rev Res. 2015;30(1):94-7.
16. Foissner W, Agatha S, Berger H. Soil ciliates (Protozoa,
Ciliophora) from Namibia (Southwest Africa), with
emphasis on two contrasting environments, the Etosha
Region and the Namib Desert. Denisia. 2002;5:1-1459.
[access 2019 nov 20]. Avaliable in: http://www.wfoissner.
at/data_prot/Foissner_etal_2002_1-1063.pdf
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ciliates yielding reproducible results. Europ J Protistol.
1995;31(4):372–82. https://dx.doi.org/10.1016/S0932-
4739(11)80449-9
18. Berger H. Monograph of the Oxytrichidae (Ciliophora,
Hypotrichia). In: Dumont HI, Werger MJA (editors).
Monographiae Biologicae, vol. 78. Springer Science;
1999.p.1-1080. https://doi.org/10.1007/978-94-011-
4637-1
19. Berger H. Monograph of the Gonostomatidae and
Kahliellidae (Ciliophora, Hypotricha). In: Monographiae
Biologicae, vol. 90, Springer Science; 2011.p.1-741.
https://10.1007/978-94-007-455-8
20. Foissner W, Berger H. A user-friendly guide to ciliates
(Protozoa, Ciliophora) commonly used by hydrobiologists
as bioindicators in rivers, lakes and waste water, with
notes in their ecology. Freshw Biol. 1996;35(2):375-482.
https://doi.org/10.1111/j.1365-2427.1996.tb01775.x
21. Foissner W, Blatterer H, Berger H, Kohmann F.
Taxonomische und ökologische revision der ciliaten des
Saprobiensystems. Band I: Cyrtophorida, Oligotrichida,
Hypotrichia, Colpodea. Informationsberichte des
Bayerischen Landesamtes für Wasserwirtschaft; 1991.p.1-
478 [access 2019 mai 09]. Avaliable in: https://www.
protozoology.com/ciliateatlas-I/
22. Foissner W, Berger H, Kohmann F. Taxonomische und
ökologische revision der ciliaten des Saprobiensystems.
Band II: Peritrichia, Heterotrichida, Odontostomatida.
Informationsberichtedes Bayer. Landesamtes für
Wasserwirtschaft; 1992.p.502. [access 2019 maio 09].
Avaliable in: https://www.protozoology.com/ciliateatlas/
23. Foissner W, Berger H, Kohmann F. Taxonomische und
ökologische revision der ciliaten des Saprobiensystems –
Band III: Hymenostomata, Prostomatida, Nassulida.
Informationsberichtedes Bayer. Landesamtes für
Wasserwirtschaft; 1994.p.548.[access 2019 mai 09].
Avaliable in: https://www.protozoology.com/
ciliateatlas-III/
24. Lynn DH, Small EB. Phylum Ciliophora. In: Lee JJ,
Bradbury PC, Leedale GF (editors.). An illustrated
guide to the protozoa. Lawrence (KS): Society of
Protozoologists; 2002.p.371-656.
25. Mohamed MA, Siddig EE, Elaagip AH, Edris AMM,
Nasr AA. Parasitic contamination of fresh vegetables
sold at central markets in Khartoum state, Sudan. Ann
Clin Microbiol Antimicrob. 2016;15:17. https://doi.
org/10.1186/s12941-016-0133-5
26. Bichai F, Dullemont Y, Hijnen W, Barbeau B. Predation
and transport of persistent pathogens in GAC and
slow sand filters: A threat to drinking water safety?
Water Res. 2014;64:296-308. https://doi.org/10.1016/j.
watres.2014.07.005
27. Chavatte N, Baré J, Lambrecht E, Van Damme I,
Vaerewijck M, Sabbe K et al. Co-occurrence of
free-living protozoa and foodborne pathogens on
dishcloths: implications for food safety. Int J Food
Microbiol. 2014;191:89-96. https://doi.org/10.1016/j.
ijfoodmicro.2014.08.030
28. Nadhanan RR, Thomas CJ. Colpoda secrete viable
Listeria monocytogenes within faecal pellets.
Environ Microbiol. 2014;16(2),396-404. https://doi.
org/10.1111/1462-2920.12230
29. Fayer R, Trout JM, Walsh E, Cole R. Rotifers
ingest oocysts of Cryptosporidium parvum. J
Eukaryot Microbiol. 2000;47(2):161-3. https://doi.
org/10.1111/j.1550-7408.2000.tb00026.x
30. Stott R, May E, Matsushita E, Warren A. Protozoan
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Copyright (c) 2020 Taís Rondello Bonatti, Sandra Yamashiro, Pedro Ferreira de Castro Santana e Freitas, Isabel Cristina Vidal Siqueira-Castro, Regina Maura Bueno Franco
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