Palavras-chave
Infecções por Neisseria meningitidis grupo B
Imunoeletroforose cruzada
Métodos diagnósticos
Imunoeletroforose cruzada
Métodos diagnósticos
Como Citar
1.
das Graças Adelino Alkmin M, Maria Landgraf I, de Fátima Paiva Vieira M. Contribuição da Imunoeletroforese cruzada em líquido cefalorraquidiano e/ou soro no diagnóstico de infecções Neisseria meningitidis grupo B no Brasil. Rev Inst Adolfo Lutz [Internet]. 28º de junho de 1996 [citado 15º de maio de 2024];56(1):13-7. Disponível em: https://periodicos.saude.sp.gov.br/RIAL/article/view/36568
Resumo
Irnunoeletroforesc cruzada (IEC) foi empregada para pesquisar a presença de antígeno de Neisseria meningitidis grupo B em 185 amostras de líquido cefalorraquidiano (LCR) e/ou 125 amostras de soro. Dentre as amostras de LCR, 103 (55,7%) foram positivas apenas através da cultura e 36 (19,5%) apenas na reação de IEe. Em 34 (18,4%) destas amostras, ambos os métodos apresentaram resultados positivos. Nos casos em que a cultura foi negativa, a IEC em amostras de LCR e/ou soro contribuiu para o diagnóstico da doença meningocócica grupo B em 26,7% dos casos, sendo que em 99,1% deles já havia sido iniciada terapia antibacteriana. Considerando os resultados positivos obtidos apenas na IEC do soro, foi observada uma percentagem de 5,6%. A contribuição da IEC em LCR e/ou soro foi signficativa.
Referências
1. ALKMIN, M.GA; SHIMIZU, S.H.; LANDGRAF, l.M.; GASPARI, E.N. & MELLES, C.E.A. - Production anda immunochemical characterization of Neisseria meningitidis group B antiserum for the diagnosis of purulent meningitis. Brazilian. J. Med. Biol. Res., 27:1627-1634, 1994.
2. ALKMIN, M.G.A.; MELLES, C.E.A. & LANGRAF, I.M. - Contribution of latex agglutination test for the diagnosis of pretreated meningitis. Serodiagn. Immunother. Infect. Disease,7: 141-142, 1995.
3. ALKMIN, M.G.A.; LANDGRAF, I.M.L., VIEIRA, M.F.P.; CAMARGO, M.C.C. & GONÇALVES, M.I.C. - Diagnosis of bacterial meningitis and septicemia by serum counterimmunoelectrophoresis, Brasilian 1. Med. Biol. Res. 28: 1065-1068, 1995.
4. BALLOWS, A.(Editor)-Manual of Clinical Microbiologv, 5th ed., Washington, American Society for Microbiology, 1991.
5. COLL, P.; BORCHE, L.; AUSINA, v, MIRELIS, B. & PRATS, G. - Dot-Immunobinding assay with a monoc1onal antibody for detection of group B meningococcal antigen. Eur. J. Clin. Microhiol., 5:44-46, 1986.
6. FASOLA, E. & FERRIERT, P. - Laboratory diagnostic methods for central nervous system infections. Neurosurg. Clin. North Am., 3:279289, 1992.
7. FINNE,l,MAKELA,P.H. &LEINONEN,M. -Antigenic similaritics between brain components and bacteria causing meningitis. Lancet, i:355-357, 1983.
8. GHANASSTA, l.P.; SLIM, A.; BERGNONEBEREZIN, E. & MODAl, J. - Failure of diagnosing group B meningococca1 meningitis by immunoeletrophoresis. Scand. J. Infect. Dis., 9:313-314, 1977.
9. GOLD, R. - Clinical aspects of meningococcal disease. ln: Vedros, N.A. - Evolution of meningococcal disease, CRC, Boca Raton, v.2, p. 69-97, 1987.
10. GOTSCHLICH, E.C.; LIU, T.Y. & ARNSTEIN, M.S. - Human immunity to the meningococcus. III. Preparation and immunochemical properties of the group A, group B and C meningococcal polysaccharides. 1. Exp. Med., 129: 1349-1365, 1969.
11. GREENWOOD, BM; WHITTLE, H.C. & DOMINIC-RAlKOVIC, O. - Counter-current immunoelectrophoresis in the diagnosis of meningococcal infections. Lancei, 2: 519-521 ,1971.
12. JONES, D.M. & ABBOTT, l.D. - Diagnosis of pyogenic meningitis. Lancet, 2:257, 1976.
13. KALDOR, J.,ASZNOVICZ, R. & BUIST, D.G.P. - Latex agglutination in diagnosis of bacterial infections with specia1 reference to patients with meningitis and septicemia. A. J. Clin. Path., 68: 284-289, 1977.
14. LANDGRAF, LM.; ALKIMIN, M.GA &VIEIRA, M.F.P. - Bacterial antigen detection in cerebrospinal fluid by the latex agg1utination test. Rev. Inst. Med. Trop. São Paulo, 37: 257-260, 1995.
15. MELLES, C.E.A.; LEE, LM.L. & TAUNAY, A.E. - Pesquisa de antibacterianos no líquido cefalorraquidiano. Rev. Inst. Adolfo Lutr, 44: 155-159, 1984.
16. MORAIS, J.C., PERKINS, B.A; CAMARGO, M.C.C.; HIDALGO, N.T.R.; BARBOSA, H.A.B.; SACCHI, C.T.; LANDGRAF, I.M; GATTAS, VL; VASCONCELOS, H.G.; ALIKAYTIS, B.D; WENGER, lD. & BROOME, c.v. - Protective efficacy of a serogroup B meningococcal vaccine in São Paulo, Brazil. Lancet, 340: 1074-1078, 1992.
17. PANACHÃO, M.R.I.; KALLÁS, E.G.; BARBOSA, H.A., OKAI, M.r.C.G.; CAMARGO, M.C.C.; SACCHI, c.r., GORLA, M.C.O. & HIDALGO, N.T.R. - Group B meningococcal disease in São Paulo, Brazil: An epidmiological and microbiological study. In: Neisseria 94 Procedings of the Ninth International Pathogenic Neisseria Conference. Evans, J.S.; Yost, S.E.; Maiden, lVl.C.J. & Feavers, LM., eds.- Winchester, England. The Guidhall, p. 395, 1994.
18. SCHWARTZ, B.; MOORE, P.S. & BROOME, c.v.- Global epidemiology of meningococcal disease clin. Microbiol. Rev., 2: (suppl.I): SI18-S124,1989.
19. TILTON, R.C.; DIAS, F.& RYAN, R.W. Comparative evaluation of three commerciaJ products and counterimmunoelectrophoresis for the detection of antigens in cerebrospinal t1uid. J.Clin. Microbiol., 20: 231-234,1984.
2. ALKMIN, M.G.A.; MELLES, C.E.A. & LANGRAF, I.M. - Contribution of latex agglutination test for the diagnosis of pretreated meningitis. Serodiagn. Immunother. Infect. Disease,7: 141-142, 1995.
3. ALKMIN, M.G.A.; LANDGRAF, I.M.L., VIEIRA, M.F.P.; CAMARGO, M.C.C. & GONÇALVES, M.I.C. - Diagnosis of bacterial meningitis and septicemia by serum counterimmunoelectrophoresis, Brasilian 1. Med. Biol. Res. 28: 1065-1068, 1995.
4. BALLOWS, A.(Editor)-Manual of Clinical Microbiologv, 5th ed., Washington, American Society for Microbiology, 1991.
5. COLL, P.; BORCHE, L.; AUSINA, v, MIRELIS, B. & PRATS, G. - Dot-Immunobinding assay with a monoc1onal antibody for detection of group B meningococcal antigen. Eur. J. Clin. Microhiol., 5:44-46, 1986.
6. FASOLA, E. & FERRIERT, P. - Laboratory diagnostic methods for central nervous system infections. Neurosurg. Clin. North Am., 3:279289, 1992.
7. FINNE,l,MAKELA,P.H. &LEINONEN,M. -Antigenic similaritics between brain components and bacteria causing meningitis. Lancet, i:355-357, 1983.
8. GHANASSTA, l.P.; SLIM, A.; BERGNONEBEREZIN, E. & MODAl, J. - Failure of diagnosing group B meningococca1 meningitis by immunoeletrophoresis. Scand. J. Infect. Dis., 9:313-314, 1977.
9. GOLD, R. - Clinical aspects of meningococcal disease. ln: Vedros, N.A. - Evolution of meningococcal disease, CRC, Boca Raton, v.2, p. 69-97, 1987.
10. GOTSCHLICH, E.C.; LIU, T.Y. & ARNSTEIN, M.S. - Human immunity to the meningococcus. III. Preparation and immunochemical properties of the group A, group B and C meningococcal polysaccharides. 1. Exp. Med., 129: 1349-1365, 1969.
11. GREENWOOD, BM; WHITTLE, H.C. & DOMINIC-RAlKOVIC, O. - Counter-current immunoelectrophoresis in the diagnosis of meningococcal infections. Lancei, 2: 519-521 ,1971.
12. JONES, D.M. & ABBOTT, l.D. - Diagnosis of pyogenic meningitis. Lancet, 2:257, 1976.
13. KALDOR, J.,ASZNOVICZ, R. & BUIST, D.G.P. - Latex agglutination in diagnosis of bacterial infections with specia1 reference to patients with meningitis and septicemia. A. J. Clin. Path., 68: 284-289, 1977.
14. LANDGRAF, LM.; ALKIMIN, M.GA &VIEIRA, M.F.P. - Bacterial antigen detection in cerebrospinal fluid by the latex agg1utination test. Rev. Inst. Med. Trop. São Paulo, 37: 257-260, 1995.
15. MELLES, C.E.A.; LEE, LM.L. & TAUNAY, A.E. - Pesquisa de antibacterianos no líquido cefalorraquidiano. Rev. Inst. Adolfo Lutr, 44: 155-159, 1984.
16. MORAIS, J.C., PERKINS, B.A; CAMARGO, M.C.C.; HIDALGO, N.T.R.; BARBOSA, H.A.B.; SACCHI, C.T.; LANDGRAF, I.M; GATTAS, VL; VASCONCELOS, H.G.; ALIKAYTIS, B.D; WENGER, lD. & BROOME, c.v. - Protective efficacy of a serogroup B meningococcal vaccine in São Paulo, Brazil. Lancet, 340: 1074-1078, 1992.
17. PANACHÃO, M.R.I.; KALLÁS, E.G.; BARBOSA, H.A., OKAI, M.r.C.G.; CAMARGO, M.C.C.; SACCHI, c.r., GORLA, M.C.O. & HIDALGO, N.T.R. - Group B meningococcal disease in São Paulo, Brazil: An epidmiological and microbiological study. In: Neisseria 94 Procedings of the Ninth International Pathogenic Neisseria Conference. Evans, J.S.; Yost, S.E.; Maiden, lVl.C.J. & Feavers, LM., eds.- Winchester, England. The Guidhall, p. 395, 1994.
18. SCHWARTZ, B.; MOORE, P.S. & BROOME, c.v.- Global epidemiology of meningococcal disease clin. Microbiol. Rev., 2: (suppl.I): SI18-S124,1989.
19. TILTON, R.C.; DIAS, F.& RYAN, R.W. Comparative evaluation of three commerciaJ products and counterimmunoelectrophoresis for the detection of antigens in cerebrospinal t1uid. J.Clin. Microbiol., 20: 231-234,1984.
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
Copyright (c) 1996 Maria das Graças Adelino Alkmin, Ilka Maria Landgraf, Maria de Fátima Paiva Vieira
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