Keywords
Neisseria meningitidis group B infections
counterimmunoelectrophoresis
diagnostic methods
counterimmunoelectrophoresis
diagnostic methods
How to Cite
1.
das Graças Adelino Alkmin M, Maria Landgraf I, de Fátima Paiva Vieira M. Contribution of counterimmunoelectrophoresis in cerebrospinal fluid and/or serum in the diagnosis of Neisseria meningitidis group B infections in Brazil. Rev Inst Adolfo Lutz [Internet]. 1996 Jun. 28 [cited 2024 Jul. 3];56(1):13-7. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/36568
Abstract
Countcrimmunoelcctrophoresis (CIE) has been used to show the presence of
Neisseria meningitidis group B antigcn in 185 cerebrospina1 fluid (CSF) and/or 125 serum samples. From the CSF samples, 103 (55.7%) wcre only cu1ture positive and 36 (19.5%) showed only positive ClE reactions. In 34 (18.4%) of thesc samples both methods had positive results. Among patients who had negative CSF culture, ClE in CSF and/or serum contributed for the diagnosis of group B meningococcus disease in26. 70/" from which 99.1 % had already received previous antibacterial therapy. Considering lhe on1y positive results by ClE in serum, it was observcd a percentage of 5.6%. The contribution of ClE in CSF and/or serum was significant.
References
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2. ALKMIN, M.G.A.; MELLES, C.E.A. & LANGRAF, I.M. - Contribution of latex agglutination test for the diagnosis of pretreated meningitis. Serodiagn. Immunother. Infect. Disease,7: 141-142, 1995.
3. ALKMIN, M.G.A.; LANDGRAF, I.M.L., VIEIRA, M.F.P.; CAMARGO, M.C.C. & GONÇALVES, M.I.C. - Diagnosis of bacterial meningitis and septicemia by serum counterimmunoelectrophoresis, Brasilian 1. Med. Biol. Res. 28: 1065-1068, 1995.
4. BALLOWS, A.(Editor)-Manual of Clinical Microbiologv, 5th ed., Washington, American Society for Microbiology, 1991.
5. COLL, P.; BORCHE, L.; AUSINA, v, MIRELIS, B. & PRATS, G. - Dot-Immunobinding assay with a monoc1onal antibody for detection of group B meningococcal antigen. Eur. J. Clin. Microhiol., 5:44-46, 1986.
6. FASOLA, E. & FERRIERT, P. - Laboratory diagnostic methods for central nervous system infections. Neurosurg. Clin. North Am., 3:279289, 1992.
7. FINNE,l,MAKELA,P.H. &LEINONEN,M. -Antigenic similaritics between brain components and bacteria causing meningitis. Lancet, i:355-357, 1983.
8. GHANASSTA, l.P.; SLIM, A.; BERGNONEBEREZIN, E. & MODAl, J. - Failure of diagnosing group B meningococca1 meningitis by immunoeletrophoresis. Scand. J. Infect. Dis., 9:313-314, 1977.
9. GOLD, R. - Clinical aspects of meningococcal disease. ln: Vedros, N.A. - Evolution of meningococcal disease, CRC, Boca Raton, v.2, p. 69-97, 1987.
10. GOTSCHLICH, E.C.; LIU, T.Y. & ARNSTEIN, M.S. - Human immunity to the meningococcus. III. Preparation and immunochemical properties of the group A, group B and C meningococcal polysaccharides. 1. Exp. Med., 129: 1349-1365, 1969.
11. GREENWOOD, BM; WHITTLE, H.C. & DOMINIC-RAlKOVIC, O. - Counter-current immunoelectrophoresis in the diagnosis of meningococcal infections. Lancei, 2: 519-521 ,1971.
12. JONES, D.M. & ABBOTT, l.D. - Diagnosis of pyogenic meningitis. Lancet, 2:257, 1976.
13. KALDOR, J.,ASZNOVICZ, R. & BUIST, D.G.P. - Latex agglutination in diagnosis of bacterial infections with specia1 reference to patients with meningitis and septicemia. A. J. Clin. Path., 68: 284-289, 1977.
14. LANDGRAF, LM.; ALKIMIN, M.GA &VIEIRA, M.F.P. - Bacterial antigen detection in cerebrospinal fluid by the latex agg1utination test. Rev. Inst. Med. Trop. São Paulo, 37: 257-260, 1995.
15. MELLES, C.E.A.; LEE, LM.L. & TAUNAY, A.E. - Pesquisa de antibacterianos no líquido cefalorraquidiano. Rev. Inst. Adolfo Lutr, 44: 155-159, 1984.
16. MORAIS, J.C., PERKINS, B.A; CAMARGO, M.C.C.; HIDALGO, N.T.R.; BARBOSA, H.A.B.; SACCHI, C.T.; LANDGRAF, I.M; GATTAS, VL; VASCONCELOS, H.G.; ALIKAYTIS, B.D; WENGER, lD. & BROOME, c.v. - Protective efficacy of a serogroup B meningococcal vaccine in São Paulo, Brazil. Lancet, 340: 1074-1078, 1992.
17. PANACHÃO, M.R.I.; KALLÁS, E.G.; BARBOSA, H.A., OKAI, M.r.C.G.; CAMARGO, M.C.C.; SACCHI, c.r., GORLA, M.C.O. & HIDALGO, N.T.R. - Group B meningococcal disease in São Paulo, Brazil: An epidmiological and microbiological study. In: Neisseria 94 Procedings of the Ninth International Pathogenic Neisseria Conference. Evans, J.S.; Yost, S.E.; Maiden, lVl.C.J. & Feavers, LM., eds.- Winchester, England. The Guidhall, p. 395, 1994.
18. SCHWARTZ, B.; MOORE, P.S. & BROOME, c.v.- Global epidemiology of meningococcal disease clin. Microbiol. Rev., 2: (suppl.I): SI18-S124,1989.
19. TILTON, R.C.; DIAS, F.& RYAN, R.W. Comparative evaluation of three commerciaJ products and counterimmunoelectrophoresis for the detection of antigens in cerebrospinal t1uid. J.Clin. Microbiol., 20: 231-234,1984.
2. ALKMIN, M.G.A.; MELLES, C.E.A. & LANGRAF, I.M. - Contribution of latex agglutination test for the diagnosis of pretreated meningitis. Serodiagn. Immunother. Infect. Disease,7: 141-142, 1995.
3. ALKMIN, M.G.A.; LANDGRAF, I.M.L., VIEIRA, M.F.P.; CAMARGO, M.C.C. & GONÇALVES, M.I.C. - Diagnosis of bacterial meningitis and septicemia by serum counterimmunoelectrophoresis, Brasilian 1. Med. Biol. Res. 28: 1065-1068, 1995.
4. BALLOWS, A.(Editor)-Manual of Clinical Microbiologv, 5th ed., Washington, American Society for Microbiology, 1991.
5. COLL, P.; BORCHE, L.; AUSINA, v, MIRELIS, B. & PRATS, G. - Dot-Immunobinding assay with a monoc1onal antibody for detection of group B meningococcal antigen. Eur. J. Clin. Microhiol., 5:44-46, 1986.
6. FASOLA, E. & FERRIERT, P. - Laboratory diagnostic methods for central nervous system infections. Neurosurg. Clin. North Am., 3:279289, 1992.
7. FINNE,l,MAKELA,P.H. &LEINONEN,M. -Antigenic similaritics between brain components and bacteria causing meningitis. Lancet, i:355-357, 1983.
8. GHANASSTA, l.P.; SLIM, A.; BERGNONEBEREZIN, E. & MODAl, J. - Failure of diagnosing group B meningococca1 meningitis by immunoeletrophoresis. Scand. J. Infect. Dis., 9:313-314, 1977.
9. GOLD, R. - Clinical aspects of meningococcal disease. ln: Vedros, N.A. - Evolution of meningococcal disease, CRC, Boca Raton, v.2, p. 69-97, 1987.
10. GOTSCHLICH, E.C.; LIU, T.Y. & ARNSTEIN, M.S. - Human immunity to the meningococcus. III. Preparation and immunochemical properties of the group A, group B and C meningococcal polysaccharides. 1. Exp. Med., 129: 1349-1365, 1969.
11. GREENWOOD, BM; WHITTLE, H.C. & DOMINIC-RAlKOVIC, O. - Counter-current immunoelectrophoresis in the diagnosis of meningococcal infections. Lancei, 2: 519-521 ,1971.
12. JONES, D.M. & ABBOTT, l.D. - Diagnosis of pyogenic meningitis. Lancet, 2:257, 1976.
13. KALDOR, J.,ASZNOVICZ, R. & BUIST, D.G.P. - Latex agglutination in diagnosis of bacterial infections with specia1 reference to patients with meningitis and septicemia. A. J. Clin. Path., 68: 284-289, 1977.
14. LANDGRAF, LM.; ALKIMIN, M.GA &VIEIRA, M.F.P. - Bacterial antigen detection in cerebrospinal fluid by the latex agg1utination test. Rev. Inst. Med. Trop. São Paulo, 37: 257-260, 1995.
15. MELLES, C.E.A.; LEE, LM.L. & TAUNAY, A.E. - Pesquisa de antibacterianos no líquido cefalorraquidiano. Rev. Inst. Adolfo Lutr, 44: 155-159, 1984.
16. MORAIS, J.C., PERKINS, B.A; CAMARGO, M.C.C.; HIDALGO, N.T.R.; BARBOSA, H.A.B.; SACCHI, C.T.; LANDGRAF, I.M; GATTAS, VL; VASCONCELOS, H.G.; ALIKAYTIS, B.D; WENGER, lD. & BROOME, c.v. - Protective efficacy of a serogroup B meningococcal vaccine in São Paulo, Brazil. Lancet, 340: 1074-1078, 1992.
17. PANACHÃO, M.R.I.; KALLÁS, E.G.; BARBOSA, H.A., OKAI, M.r.C.G.; CAMARGO, M.C.C.; SACCHI, c.r., GORLA, M.C.O. & HIDALGO, N.T.R. - Group B meningococcal disease in São Paulo, Brazil: An epidmiological and microbiological study. In: Neisseria 94 Procedings of the Ninth International Pathogenic Neisseria Conference. Evans, J.S.; Yost, S.E.; Maiden, lVl.C.J. & Feavers, LM., eds.- Winchester, England. The Guidhall, p. 395, 1994.
18. SCHWARTZ, B.; MOORE, P.S. & BROOME, c.v.- Global epidemiology of meningococcal disease clin. Microbiol. Rev., 2: (suppl.I): SI18-S124,1989.
19. TILTON, R.C.; DIAS, F.& RYAN, R.W. Comparative evaluation of three commerciaJ products and counterimmunoelectrophoresis for the detection of antigens in cerebrospinal t1uid. J.Clin. Microbiol., 20: 231-234,1984.
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Copyright (c) 1996 Instituto Adolfo Lutz Journal
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