Palavras-chave
Ocratoxina A
café
micotoxinas
café
micotoxinas
Como Citar
1.
Prado Zanes Furlani R, M. Valente Soares L, Lauro C. de Oliveira P. Avaliação de métodos para determinação de Ocratoxina A em cafés verdes e torrados. Rev Inst Adolfo Lutz [Internet]. 30º de dezembro de 1999 [citado 4º de dezembro de 2024];58(2):87-98. Disponível em: https://periodicos.saude.sp.gov.br/RIAL/article/view/36698
Resumo
Diferentes solventes de extração, assim como técnicas para limpeza de extratos para determinação de rnicotoxinas, tanto clássicas (clarificação, partição) como aquelas envolvendo colunas para extração em fase sólida (sílica, octadecilsilil e imunoafinidade) foram avaliadas para determinação de ocratoxina A em café verde e torrado. Apenas as colunas de imunoafinidade foram capazes de remover os interferentes existentes nos extratos de café permitindo a determinação de ocratoxina A por cromatografia líquida de alta eficiência com detecção por fluorescência em níveis de 0,7 ng/g. Colunas de imunoafinidade de duas marcas diferentes foram testadas com relação à recuperação da toxina e à reutilização em outras análises. Colunas de uma marca apresentaram recuperação média de 97% no primeiro uso e acima de 70% até a quarta utilização. Colunas de uma segunda marca apresentaram recuperação média de 73% no primeiro uso e abaixo de 30% no segundo.
Referências
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2. AOAC INTERNATIONAL. Official Methods of Analysis, 163 ed., 33 rev., Gaithersburg, MD, Method 975.38, 1997.
3. BREITHOLTZ-EMANUELSSON, A.; OLSEN, M.; OSKARSSON, A; PALMINGER, L & HULT, K. Ochratoxin A in cow's milk and in human milk with corresponding human blood samples, 1. AOAC Int., 76: 842-846, 1993.
4. BUSBY JR., W.F. & WOGAN, G.N. Ochratoxins. In: Mycotoxins and Nmitoso compounds: environmental risks, Boca Raton, SHANK, R.C., 1981, vol.II, 12.129-136.
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6. DIPARTIMENTO DOGANE - Circolare n. 76 Ministerio delle Finanze -Roma, Itália, 21/03/1996.
7. DIRHEIMER, G. Mechanistic approaches to ochratoxin toxicity. Food Add. Contam., 13: 45-48, Supplement, 1996.
8. HÃGGBLOM, P.E. & GHOSH, J. Postharvest production of ochratoxin A by Aspergillus ochraceus and Penicillium viridicatum in barley with different protein levels, Appl. Environ. Microbiol., 49: 787790, 1985.
9. HOWELL, M.V. & TAYLOR, P.W. Determination of aflatoxins, ochratoxin A, and zearalenone in mixed feeds, with detection by thin layer chromatography or high performance liquid chromatography, 1. Assoc. Off. Anal. Chem., 64: 1356-1363, 1981.
10. INTERNATIONAL UNION FOR PURE AND APPLIED CHEMISTRY. Nomenclature for chromatography. Pure Appl. Chem. 65: 819-872, 1993.
11. JORGENSEN, K. Survey of pork, poultry, coffee, beer and pulses for ochratoxin A, Food Add. Contam. 15: 550-554, 1998.
12. JW Scientific Products Catalog & Reference Guide, 1992-93.
13. MORICOCHI, L. Café. [http:www.iea.sp.gov.br/ café 98.htm], 29/06/99.
14. MICCO, c. GROSSI, M.; MIRAGLIA, M. & BRERA, C. Study of the contamination by ochratoxin A of green and roasted coffee beans. Food Add. Contam" 6: 333-339, 1989,
15, MOSS, M, O, Mode of formation of ochratoxin A Food Add, Contam" 13: 5-9, Supplement, 1996.
16, NAKAJIMA, M,; TERADA, H.; HISADA, K; TSUBOUCHI, H.; YAMAMOTO,K; UDA, T; ITOH, Y & UENO, Y Determination of ochratoxin A in coffee beans and coffee products by monoclonal antibody affinity chromatography. Food Agric. Immunol., 2: 189-195, 1990.
17. PATEL, S.; HAZEL, C. M.; WINTERTON, A G. M. & GLEADLE, A E. Survey of ochratoxin A in UK retail coffees. FoodAdd. Contam., 14: 217-222, 1997.
18. PATEL, S.; HAZEL, C. M.; WINTERTON, A G. M. & MORTBY, E., Survey of ethnic foods for mycotoxins. Food Add. Contam., 13: 833-841, 1996.
19. PITET, A; TORNARE, D.; HUGGET, A & VIANI, R Liquid chromatographic determination of ochratoxin A in pure and adulterated soluble coffee using analyze immunoaffinity colurnn cleanup procedure. J. Agrie. Food Chem., 44: 35643569,1996.
20. PLESTINA, R Nephrotoxicity of ochratoxin A Food Add. Contam., 13: 49-50, Supplement, 1996.
21. POHLAND, A. E.; NESHEIM, S. & FRIEDMAN, L. Ochratoxin APure Appl. Chem. 64: 1029-1046, 1992.
22. RHÔNE-DIAGNOSTICS TECHNOLOGIES LTD. oehaprep quantitative deteetion of ocratoxin A. Instructions for use, Products codes: P14, P14B, Version P14/v21 11.08.97.
23. SCOTT, P. M. & KANHERE, S. R. Determination of ochratoxin A in beer. Food Add. Contam., 12: 591-598, 1995.
24. SHARMA, RP. & SALUNKHE, D.K Occurrence of mycotoxins in foods and feeds. in: Myeotoxins and phytoalexins, Boca Raton, FL, SHARMA, RP &, SALUNKHE, D.K, 1991, p.13-31.
25. SOARES, L.M.V. & RODRIGUES-AMAYA, D.B., Screening and quantitation of ochratoxin A in com, peanuts, beans, rice, and cassava, 1. Assoc.
Off.Anal. Chem., 68: 1128-1130,1985.
26. SPEIJERS, l.A & VAN EGMOND, H.P. Worl-dwide ochratoxin A 1eve1s in food and feeds, in: Human oehratoxieosis and its pathologies, Paris, CREPPY, E.E; CASTEGNARO, M. & DIRHEIMER, G., 1993, p. 85 - 100.
27. STEGEN, G. V. D.; lORRISSEN, D.; PITTET, A; SACCON, M.; STEINER,W.; VICENZI, M.; WINKLER, M.; ZAPP, l. & SCHLATTER, C. Screening of European coffee final products for occurrence of ochratoxin A (OTA). Food Add. Contam., 14: 211-216, 1997.
28. STUDER-ROHR, L; DIETRICH, D. R; SCHLATTER,J.; SCHLATTER, C. The occurrence of ochratoxin A in coffee. Food Chemical Toxieology,.33: 341-355, 1995.
29. TERADA, H.; TSOUBUCHI, H.; YAMAMOTO, K ;HISADA, K & SAKABE, Y Liquid chromatographic determination of ochratoxin A in coffee beans and coffee products. J. Assoe. Off. Anal. Chem., 69: 960-964, 1986.
30. TRUCKSESS, M. W.; CHOU, l.P.; YOUNG,K & PAGE,S.W. Determination and survey of ochratoxin A in barley, green coffee and roasted coffee. FDA Seienee Forum Poster Abstract, [http://vm.cfsan.fda.gov/=frLforum97197A30 .htm] , 1997.
31. TSUBOUCHI, H.; TERADA, H.; YAMAMOTO, K; HISADA, K & SAKABE, Y Ochratoxin A found in commercial roast coffee. J. Agrie. Food Chem., 36: 540-542, 1988.
32. VALENTA, H. & GOLL, M., Determination of ochratoxin A in regional samples of cow's milk from Germany. Food Add. Contam., l3: 669-676, 1996.
33. VAN EGMOND, H. P. Analytical methodology and regulations for ochratoxin A Food Add. Contam., 13: 11-l3, Supplement, 1996.26.
34. VERARDI, G.; ROSNER, H. Some Reflections on Establishing a Community Legislation on Mycotoxins. Natural Toxins, 3: 337-340, 1995.
35. ZIMMERLI, B. & DICK, R Study to repeated Use of commercial immunoaffinity columns. Mitt. Gebiete Lebensm. Hyg., 87: 732-742, 1996.
36. ZIMMERLI, B. & DICK, R. Ochratoxin A in table wine and grape-juice: occurrence and risk assessment, Food Add. Contam., 6: 655-668, 1996.
2249, 1980.
2. AOAC INTERNATIONAL. Official Methods of Analysis, 163 ed., 33 rev., Gaithersburg, MD, Method 975.38, 1997.
3. BREITHOLTZ-EMANUELSSON, A.; OLSEN, M.; OSKARSSON, A; PALMINGER, L & HULT, K. Ochratoxin A in cow's milk and in human milk with corresponding human blood samples, 1. AOAC Int., 76: 842-846, 1993.
4. BUSBY JR., W.F. & WOGAN, G.N. Ochratoxins. In: Mycotoxins and Nmitoso compounds: environmental risks, Boca Raton, SHANK, R.C., 1981, vol.II, 12.129-136.
5. CANTAFORA, A; GROSSI, M.; MIRAGLIA M. & BENELLI, L., Determination of ochratoxin A in coffee beans using reversed-phase high perforrnance liquid chromatography, Riv. Soe. Ital. Alimen., 12: 103-108, 1983.
6. DIPARTIMENTO DOGANE - Circolare n. 76 Ministerio delle Finanze -Roma, Itália, 21/03/1996.
7. DIRHEIMER, G. Mechanistic approaches to ochratoxin toxicity. Food Add. Contam., 13: 45-48, Supplement, 1996.
8. HÃGGBLOM, P.E. & GHOSH, J. Postharvest production of ochratoxin A by Aspergillus ochraceus and Penicillium viridicatum in barley with different protein levels, Appl. Environ. Microbiol., 49: 787790, 1985.
9. HOWELL, M.V. & TAYLOR, P.W. Determination of aflatoxins, ochratoxin A, and zearalenone in mixed feeds, with detection by thin layer chromatography or high performance liquid chromatography, 1. Assoc. Off. Anal. Chem., 64: 1356-1363, 1981.
10. INTERNATIONAL UNION FOR PURE AND APPLIED CHEMISTRY. Nomenclature for chromatography. Pure Appl. Chem. 65: 819-872, 1993.
11. JORGENSEN, K. Survey of pork, poultry, coffee, beer and pulses for ochratoxin A, Food Add. Contam. 15: 550-554, 1998.
12. JW Scientific Products Catalog & Reference Guide, 1992-93.
13. MORICOCHI, L. Café. [http:www.iea.sp.gov.br/ café 98.htm], 29/06/99.
14. MICCO, c. GROSSI, M.; MIRAGLIA, M. & BRERA, C. Study of the contamination by ochratoxin A of green and roasted coffee beans. Food Add. Contam" 6: 333-339, 1989,
15, MOSS, M, O, Mode of formation of ochratoxin A Food Add, Contam" 13: 5-9, Supplement, 1996.
16, NAKAJIMA, M,; TERADA, H.; HISADA, K; TSUBOUCHI, H.; YAMAMOTO,K; UDA, T; ITOH, Y & UENO, Y Determination of ochratoxin A in coffee beans and coffee products by monoclonal antibody affinity chromatography. Food Agric. Immunol., 2: 189-195, 1990.
17. PATEL, S.; HAZEL, C. M.; WINTERTON, A G. M. & GLEADLE, A E. Survey of ochratoxin A in UK retail coffees. FoodAdd. Contam., 14: 217-222, 1997.
18. PATEL, S.; HAZEL, C. M.; WINTERTON, A G. M. & MORTBY, E., Survey of ethnic foods for mycotoxins. Food Add. Contam., 13: 833-841, 1996.
19. PITET, A; TORNARE, D.; HUGGET, A & VIANI, R Liquid chromatographic determination of ochratoxin A in pure and adulterated soluble coffee using analyze immunoaffinity colurnn cleanup procedure. J. Agrie. Food Chem., 44: 35643569,1996.
20. PLESTINA, R Nephrotoxicity of ochratoxin A Food Add. Contam., 13: 49-50, Supplement, 1996.
21. POHLAND, A. E.; NESHEIM, S. & FRIEDMAN, L. Ochratoxin APure Appl. Chem. 64: 1029-1046, 1992.
22. RHÔNE-DIAGNOSTICS TECHNOLOGIES LTD. oehaprep quantitative deteetion of ocratoxin A. Instructions for use, Products codes: P14, P14B, Version P14/v21 11.08.97.
23. SCOTT, P. M. & KANHERE, S. R. Determination of ochratoxin A in beer. Food Add. Contam., 12: 591-598, 1995.
24. SHARMA, RP. & SALUNKHE, D.K Occurrence of mycotoxins in foods and feeds. in: Myeotoxins and phytoalexins, Boca Raton, FL, SHARMA, RP &, SALUNKHE, D.K, 1991, p.13-31.
25. SOARES, L.M.V. & RODRIGUES-AMAYA, D.B., Screening and quantitation of ochratoxin A in com, peanuts, beans, rice, and cassava, 1. Assoc.
Off.Anal. Chem., 68: 1128-1130,1985.
26. SPEIJERS, l.A & VAN EGMOND, H.P. Worl-dwide ochratoxin A 1eve1s in food and feeds, in: Human oehratoxieosis and its pathologies, Paris, CREPPY, E.E; CASTEGNARO, M. & DIRHEIMER, G., 1993, p. 85 - 100.
27. STEGEN, G. V. D.; lORRISSEN, D.; PITTET, A; SACCON, M.; STEINER,W.; VICENZI, M.; WINKLER, M.; ZAPP, l. & SCHLATTER, C. Screening of European coffee final products for occurrence of ochratoxin A (OTA). Food Add. Contam., 14: 211-216, 1997.
28. STUDER-ROHR, L; DIETRICH, D. R; SCHLATTER,J.; SCHLATTER, C. The occurrence of ochratoxin A in coffee. Food Chemical Toxieology,.33: 341-355, 1995.
29. TERADA, H.; TSOUBUCHI, H.; YAMAMOTO, K ;HISADA, K & SAKABE, Y Liquid chromatographic determination of ochratoxin A in coffee beans and coffee products. J. Assoe. Off. Anal. Chem., 69: 960-964, 1986.
30. TRUCKSESS, M. W.; CHOU, l.P.; YOUNG,K & PAGE,S.W. Determination and survey of ochratoxin A in barley, green coffee and roasted coffee. FDA Seienee Forum Poster Abstract, [http://vm.cfsan.fda.gov/=frLforum97197A30 .htm] , 1997.
31. TSUBOUCHI, H.; TERADA, H.; YAMAMOTO, K; HISADA, K & SAKABE, Y Ochratoxin A found in commercial roast coffee. J. Agrie. Food Chem., 36: 540-542, 1988.
32. VALENTA, H. & GOLL, M., Determination of ochratoxin A in regional samples of cow's milk from Germany. Food Add. Contam., l3: 669-676, 1996.
33. VAN EGMOND, H. P. Analytical methodology and regulations for ochratoxin A Food Add. Contam., 13: 11-l3, Supplement, 1996.26.
34. VERARDI, G.; ROSNER, H. Some Reflections on Establishing a Community Legislation on Mycotoxins. Natural Toxins, 3: 337-340, 1995.
35. ZIMMERLI, B. & DICK, R Study to repeated Use of commercial immunoaffinity columns. Mitt. Gebiete Lebensm. Hyg., 87: 732-742, 1996.
36. ZIMMERLI, B. & DICK, R. Ochratoxin A in table wine and grape-juice: occurrence and risk assessment, Food Add. Contam., 6: 655-668, 1996.
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
Copyright (c) 1999 Regina Prado Zanes Furlani, Lucia M. Valente Soares, Paulo Lauro C. de Oliveira
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