Keywords
Ochratoxin A
coffee
mycotoxins
coffee
mycotoxins
How to Cite
1.
Prado Zanes Furlani R, M. Valente Soares L, Lauro C. de Oliveira P. Evaluation of methods for Ochratoxin A determination in green and roasted coffees. Rev Inst Adolfo Lutz [Internet]. 1999 Dec. 30 [cited 2024 May 16];58(2):87-98. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/36698
Abstract
Different extraction solvents and cIeanup techniques for mycotoxins determination were evaluated for green and roasted coffee. Classical techniques such as partition and cIarification as well as solid phase extraction (silica, octadecylsilyl, cianopropyl and immunoaffinity columns) were tested. Only immunoaffinity columns were capable to effectively remove interferents frorn coffee extracts allowing ochratoxin A to be determined by HPLC and fluorescence detection at levels of 0,7 ng/g. Two commercial brands of immunoaffinity columns were evaluated for ochratoxin A recovery and repeated use. Average recovery for one brand was 97% and was above 70% on the fourth use. Average recovery for a second brand was 73% on the first use and below 30% on the second use.
References
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16, NAKAJIMA, M,; TERADA, H.; HISADA, K; TSUBOUCHI, H.; YAMAMOTO,K; UDA, T; ITOH, Y & UENO, Y Determination of ochratoxin A in coffee beans and coffee products by monoclonal antibody affinity chromatography. Food Agric. Immunol., 2: 189-195, 1990.
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19. PITET, A; TORNARE, D.; HUGGET, A & VIANI, R Liquid chromatographic determination of ochratoxin A in pure and adulterated soluble coffee using analyze immunoaffinity colurnn cleanup procedure. J. Agrie. Food Chem., 44: 35643569,1996.
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21. POHLAND, A. E.; NESHEIM, S. & FRIEDMAN, L. Ochratoxin APure Appl. Chem. 64: 1029-1046, 1992.
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23. SCOTT, P. M. & KANHERE, S. R. Determination of ochratoxin A in beer. Food Add. Contam., 12: 591-598, 1995.
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25. SOARES, L.M.V. & RODRIGUES-AMAYA, D.B., Screening and quantitation of ochratoxin A in com, peanuts, beans, rice, and cassava, 1. Assoc.
Off.Anal. Chem., 68: 1128-1130,1985.
26. SPEIJERS, l.A & VAN EGMOND, H.P. Worl-dwide ochratoxin A 1eve1s in food and feeds, in: Human oehratoxieosis and its pathologies, Paris, CREPPY, E.E; CASTEGNARO, M. & DIRHEIMER, G., 1993, p. 85 - 100.
27. STEGEN, G. V. D.; lORRISSEN, D.; PITTET, A; SACCON, M.; STEINER,W.; VICENZI, M.; WINKLER, M.; ZAPP, l. & SCHLATTER, C. Screening of European coffee final products for occurrence of ochratoxin A (OTA). Food Add. Contam., 14: 211-216, 1997.
28. STUDER-ROHR, L; DIETRICH, D. R; SCHLATTER,J.; SCHLATTER, C. The occurrence of ochratoxin A in coffee. Food Chemical Toxieology,.33: 341-355, 1995.
29. TERADA, H.; TSOUBUCHI, H.; YAMAMOTO, K ;HISADA, K & SAKABE, Y Liquid chromatographic determination of ochratoxin A in coffee beans and coffee products. J. Assoe. Off. Anal. Chem., 69: 960-964, 1986.
30. TRUCKSESS, M. W.; CHOU, l.P.; YOUNG,K & PAGE,S.W. Determination and survey of ochratoxin A in barley, green coffee and roasted coffee. FDA Seienee Forum Poster Abstract, [http://vm.cfsan.fda.gov/=frLforum97197A30 .htm] , 1997.
31. TSUBOUCHI, H.; TERADA, H.; YAMAMOTO, K; HISADA, K & SAKABE, Y Ochratoxin A found in commercial roast coffee. J. Agrie. Food Chem., 36: 540-542, 1988.
32. VALENTA, H. & GOLL, M., Determination of ochratoxin A in regional samples of cow's milk from Germany. Food Add. Contam., l3: 669-676, 1996.
33. VAN EGMOND, H. P. Analytical methodology and regulations for ochratoxin A Food Add. Contam., 13: 11-l3, Supplement, 1996.26.
34. VERARDI, G.; ROSNER, H. Some Reflections on Establishing a Community Legislation on Mycotoxins. Natural Toxins, 3: 337-340, 1995.
35. ZIMMERLI, B. & DICK, R Study to repeated Use of commercial immunoaffinity columns. Mitt. Gebiete Lebensm. Hyg., 87: 732-742, 1996.
36. ZIMMERLI, B. & DICK, R. Ochratoxin A in table wine and grape-juice: occurrence and risk assessment, Food Add. Contam., 6: 655-668, 1996.
2249, 1980.
2. AOAC INTERNATIONAL. Official Methods of Analysis, 163 ed., 33 rev., Gaithersburg, MD, Method 975.38, 1997.
3. BREITHOLTZ-EMANUELSSON, A.; OLSEN, M.; OSKARSSON, A; PALMINGER, L & HULT, K. Ochratoxin A in cow's milk and in human milk with corresponding human blood samples, 1. AOAC Int., 76: 842-846, 1993.
4. BUSBY JR., W.F. & WOGAN, G.N. Ochratoxins. In: Mycotoxins and Nmitoso compounds: environmental risks, Boca Raton, SHANK, R.C., 1981, vol.II, 12.129-136.
5. CANTAFORA, A; GROSSI, M.; MIRAGLIA M. & BENELLI, L., Determination of ochratoxin A in coffee beans using reversed-phase high perforrnance liquid chromatography, Riv. Soe. Ital. Alimen., 12: 103-108, 1983.
6. DIPARTIMENTO DOGANE - Circolare n. 76 Ministerio delle Finanze -Roma, Itália, 21/03/1996.
7. DIRHEIMER, G. Mechanistic approaches to ochratoxin toxicity. Food Add. Contam., 13: 45-48, Supplement, 1996.
8. HÃGGBLOM, P.E. & GHOSH, J. Postharvest production of ochratoxin A by Aspergillus ochraceus and Penicillium viridicatum in barley with different protein levels, Appl. Environ. Microbiol., 49: 787790, 1985.
9. HOWELL, M.V. & TAYLOR, P.W. Determination of aflatoxins, ochratoxin A, and zearalenone in mixed feeds, with detection by thin layer chromatography or high performance liquid chromatography, 1. Assoc. Off. Anal. Chem., 64: 1356-1363, 1981.
10. INTERNATIONAL UNION FOR PURE AND APPLIED CHEMISTRY. Nomenclature for chromatography. Pure Appl. Chem. 65: 819-872, 1993.
11. JORGENSEN, K. Survey of pork, poultry, coffee, beer and pulses for ochratoxin A, Food Add. Contam. 15: 550-554, 1998.
12. JW Scientific Products Catalog & Reference Guide, 1992-93.
13. MORICOCHI, L. Café. [http:www.iea.sp.gov.br/ café 98.htm], 29/06/99.
14. MICCO, c. GROSSI, M.; MIRAGLIA, M. & BRERA, C. Study of the contamination by ochratoxin A of green and roasted coffee beans. Food Add. Contam" 6: 333-339, 1989,
15, MOSS, M, O, Mode of formation of ochratoxin A Food Add, Contam" 13: 5-9, Supplement, 1996.
16, NAKAJIMA, M,; TERADA, H.; HISADA, K; TSUBOUCHI, H.; YAMAMOTO,K; UDA, T; ITOH, Y & UENO, Y Determination of ochratoxin A in coffee beans and coffee products by monoclonal antibody affinity chromatography. Food Agric. Immunol., 2: 189-195, 1990.
17. PATEL, S.; HAZEL, C. M.; WINTERTON, A G. M. & GLEADLE, A E. Survey of ochratoxin A in UK retail coffees. FoodAdd. Contam., 14: 217-222, 1997.
18. PATEL, S.; HAZEL, C. M.; WINTERTON, A G. M. & MORTBY, E., Survey of ethnic foods for mycotoxins. Food Add. Contam., 13: 833-841, 1996.
19. PITET, A; TORNARE, D.; HUGGET, A & VIANI, R Liquid chromatographic determination of ochratoxin A in pure and adulterated soluble coffee using analyze immunoaffinity colurnn cleanup procedure. J. Agrie. Food Chem., 44: 35643569,1996.
20. PLESTINA, R Nephrotoxicity of ochratoxin A Food Add. Contam., 13: 49-50, Supplement, 1996.
21. POHLAND, A. E.; NESHEIM, S. & FRIEDMAN, L. Ochratoxin APure Appl. Chem. 64: 1029-1046, 1992.
22. RHÔNE-DIAGNOSTICS TECHNOLOGIES LTD. oehaprep quantitative deteetion of ocratoxin A. Instructions for use, Products codes: P14, P14B, Version P14/v21 11.08.97.
23. SCOTT, P. M. & KANHERE, S. R. Determination of ochratoxin A in beer. Food Add. Contam., 12: 591-598, 1995.
24. SHARMA, RP. & SALUNKHE, D.K Occurrence of mycotoxins in foods and feeds. in: Myeotoxins and phytoalexins, Boca Raton, FL, SHARMA, RP &, SALUNKHE, D.K, 1991, p.13-31.
25. SOARES, L.M.V. & RODRIGUES-AMAYA, D.B., Screening and quantitation of ochratoxin A in com, peanuts, beans, rice, and cassava, 1. Assoc.
Off.Anal. Chem., 68: 1128-1130,1985.
26. SPEIJERS, l.A & VAN EGMOND, H.P. Worl-dwide ochratoxin A 1eve1s in food and feeds, in: Human oehratoxieosis and its pathologies, Paris, CREPPY, E.E; CASTEGNARO, M. & DIRHEIMER, G., 1993, p. 85 - 100.
27. STEGEN, G. V. D.; lORRISSEN, D.; PITTET, A; SACCON, M.; STEINER,W.; VICENZI, M.; WINKLER, M.; ZAPP, l. & SCHLATTER, C. Screening of European coffee final products for occurrence of ochratoxin A (OTA). Food Add. Contam., 14: 211-216, 1997.
28. STUDER-ROHR, L; DIETRICH, D. R; SCHLATTER,J.; SCHLATTER, C. The occurrence of ochratoxin A in coffee. Food Chemical Toxieology,.33: 341-355, 1995.
29. TERADA, H.; TSOUBUCHI, H.; YAMAMOTO, K ;HISADA, K & SAKABE, Y Liquid chromatographic determination of ochratoxin A in coffee beans and coffee products. J. Assoe. Off. Anal. Chem., 69: 960-964, 1986.
30. TRUCKSESS, M. W.; CHOU, l.P.; YOUNG,K & PAGE,S.W. Determination and survey of ochratoxin A in barley, green coffee and roasted coffee. FDA Seienee Forum Poster Abstract, [http://vm.cfsan.fda.gov/=frLforum97197A30 .htm] , 1997.
31. TSUBOUCHI, H.; TERADA, H.; YAMAMOTO, K; HISADA, K & SAKABE, Y Ochratoxin A found in commercial roast coffee. J. Agrie. Food Chem., 36: 540-542, 1988.
32. VALENTA, H. & GOLL, M., Determination of ochratoxin A in regional samples of cow's milk from Germany. Food Add. Contam., l3: 669-676, 1996.
33. VAN EGMOND, H. P. Analytical methodology and regulations for ochratoxin A Food Add. Contam., 13: 11-l3, Supplement, 1996.26.
34. VERARDI, G.; ROSNER, H. Some Reflections on Establishing a Community Legislation on Mycotoxins. Natural Toxins, 3: 337-340, 1995.
35. ZIMMERLI, B. & DICK, R Study to repeated Use of commercial immunoaffinity columns. Mitt. Gebiete Lebensm. Hyg., 87: 732-742, 1996.
36. ZIMMERLI, B. & DICK, R. Ochratoxin A in table wine and grape-juice: occurrence and risk assessment, Food Add. Contam., 6: 655-668, 1996.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 1999 Instituto Adolfo Lutz Journal
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