Palavras-chave
laranja de acridina ação sobre o v´´írus
vírus herpético e vacínico
vírus herpético e vacínico
Como Citar
1.
Malachowska MI, Roth A. Ação do corante laranja de acridina sobre os vírus herpético e vacínico em culturas celulares. Rev Inst Adolfo Lutz [Internet]. 30º de dezembro de 1974 [citado 26º de dezembro de 2024];34(1-2):9-17. Disponível em: https://periodicos.saude.sp.gov.br/RIAL/article/view/37051
Resumo
Estudou-se a ação inibidora do corante foto-sensibilizante laranja de acridina (LA) sobre os vírus herpético e vacíníco. A ação conjunta do corante e luz resultou em 100% de inibição do crescimento dos vírus, quando o LA esteve em contacto durante 1 hora com os vírus. Não se observou qualquer partícula víral nas micrografias eletrônicas, enquanto que nas células inoculadas com vírus expostos à luz, mas sem LA, observaram-se numerosas partículas de vírus. A microscopia fluorescente demonstrou diminuição de ADN em células inoculadas com mistura de LA e vírus (VLA) em comparação com os controles de células normais.
Referências
1. BERTALANFFY, L. von & BICKIS, I. Identification or cytoplasmic basophilia (Ribonucleic acid) by fluorescence mtcroscopy. J. Histochem. Cytochem., 4: 481-93, 1956.
2. BOOTH, J.C. & STERN, H. - Phatodynamic inactivation of rubella virus. J. medo Microbiol., 5: 515-28, 1972.
3. DRAKE, J. W. & McGUIRE, J. - Propert.íes or r mutants or bacteriophage T4 photodynamically induced in the presence of thiopyranin and psoralen. J. Virol., 1: 260-7, 1967.
4. FELBER, T.D.; SMITH, E.B.; KNOX, J. M.; WALLIS, C. & MELNICK, J.L. - Photodynamic ínactívatíon of herpes simplex. J. amo medo Ass., 223: 289-92, 1973.
5. FRANKLIN, R.M. - The synthesis of fowl plague virus products in a proflavine-inhibited tis sue culture system. Virology, 6: 525-39, 1958.
6. FREIFELDER, D. & URETZ, R.B. - Mechanism of photainactivation of coliphage T7 sensitized by acridine orange. Virology, 30: 97-103, 1966.
7. HIATT, C.W.; KAUFMAN, E.; HELPRIN, J.J. & BARON, S. - Inactivation of viruses by the photodynamic action of toluidine blue. J. Immun. 845 480-4, 1960.
8. HILL JR., R.B.; BENSCH, K.G. & KING, D. W. - Photosensitization of nucleic acids and proteins. The photodynamic action of acridine orange on living cells in culture. Expl ceu Res., 21: 106-17, 1960.
9. LEI, J. C. - Der intravirale charakter der profla vinsensi bilisierten Photoinakti vierung des Maul-und-Klauenseuche-Virus. Arch .. exp. VetMed., 24: 125-39, 1970.
10. MALACHOWSKA, M. I. & 'ROTH, A. - Ação do ferro sobre o grupo dos poxvírus in vitro. Rev. Inst. Aâoljo Lutz, 31: 57-61, 1971.
11. MAYOR, H.D. - Cytochemical and fluorescent antibody studies on the growth of poliovirus in tissue culture. Tex. Rep. Biol. Med., 19: 106-22, 1961.
12. MOORE, C.; WALLIS, C. ; MELNICK, J. L. & KUNS, M. D. - Photodynamic treatment of herpes keratitis. Tnfection & Immunity, 5: 169-71, 1972,.
13. ORLOB, G.B. - Some effects of photosensitízmg dyes on three plant víruses. Virology, 21; 291-9, 1963.
14. ROMANOWSKAJA, L.N. et alii - K Woprosu o mecanizmie zwiazywania akridinowych Krasitieley z nukleinewymi kisletemi. Biofizika, 17: 313-36, 1972.
15. ROSS, R. W. & ORLANS, E. - The redístribution of nucleic acid and the appearance of specific antigen in HeLa cells infected with herpes vírus. J. Path. Bact., 76: 393-402, 1958.
16. SASTRY, K.S. & GORDON, M.P. - The photodynamic inactivation of tobacco mosaic virus and its ribonucleic acid by acridine orange. Biochim. biophys. Acta, 129: 32-41, 1966.
17. SCHAFFER, F.L. & HACKETT, A.J. Early events ín poliovirus-HeLa cell interaction: acridíne oranre photosensitization and detergent extractíon. Virology, 21: 124-6, 1963.
18. SHORTT, H.E. & BROOKS, A.G. - Photodynamic action of methylene blue on fixed rabies vírus. Indian J. medo Res.,
21: 581-5, 1934.
19. WALLIS, C. & MELNICK, J. L. - Photodynamíc inactivation of poliovirus. Virology, 21: 332-41, 1963.
20. WALLIS, C. & MELNICK, J. L. - Irreversible photosensitization of viruses. Virology, 23: 520-7, 1964.
21. WALLIS, C.; SCHEIRIS, C. & MELNICK, J. L. - Photodynamically inactivated vaccines prepared by growing víruses in cells contaíning neutral red. J. Immun.,
99: 1134-9, 1967.
22. WILSON, J. N. & COOPER, P. D. - Aspects of the growth of poliovirus as revealed by the photodynamic effects of neutral red and acridine orange. Virology, 21: 135-45, 1963.
2. BOOTH, J.C. & STERN, H. - Phatodynamic inactivation of rubella virus. J. medo Microbiol., 5: 515-28, 1972.
3. DRAKE, J. W. & McGUIRE, J. - Propert.íes or r mutants or bacteriophage T4 photodynamically induced in the presence of thiopyranin and psoralen. J. Virol., 1: 260-7, 1967.
4. FELBER, T.D.; SMITH, E.B.; KNOX, J. M.; WALLIS, C. & MELNICK, J.L. - Photodynamic ínactívatíon of herpes simplex. J. amo medo Ass., 223: 289-92, 1973.
5. FRANKLIN, R.M. - The synthesis of fowl plague virus products in a proflavine-inhibited tis sue culture system. Virology, 6: 525-39, 1958.
6. FREIFELDER, D. & URETZ, R.B. - Mechanism of photainactivation of coliphage T7 sensitized by acridine orange. Virology, 30: 97-103, 1966.
7. HIATT, C.W.; KAUFMAN, E.; HELPRIN, J.J. & BARON, S. - Inactivation of viruses by the photodynamic action of toluidine blue. J. Immun. 845 480-4, 1960.
8. HILL JR., R.B.; BENSCH, K.G. & KING, D. W. - Photosensitization of nucleic acids and proteins. The photodynamic action of acridine orange on living cells in culture. Expl ceu Res., 21: 106-17, 1960.
9. LEI, J. C. - Der intravirale charakter der profla vinsensi bilisierten Photoinakti vierung des Maul-und-Klauenseuche-Virus. Arch .. exp. VetMed., 24: 125-39, 1970.
10. MALACHOWSKA, M. I. & 'ROTH, A. - Ação do ferro sobre o grupo dos poxvírus in vitro. Rev. Inst. Aâoljo Lutz, 31: 57-61, 1971.
11. MAYOR, H.D. - Cytochemical and fluorescent antibody studies on the growth of poliovirus in tissue culture. Tex. Rep. Biol. Med., 19: 106-22, 1961.
12. MOORE, C.; WALLIS, C. ; MELNICK, J. L. & KUNS, M. D. - Photodynamic treatment of herpes keratitis. Tnfection & Immunity, 5: 169-71, 1972,.
13. ORLOB, G.B. - Some effects of photosensitízmg dyes on three plant víruses. Virology, 21; 291-9, 1963.
14. ROMANOWSKAJA, L.N. et alii - K Woprosu o mecanizmie zwiazywania akridinowych Krasitieley z nukleinewymi kisletemi. Biofizika, 17: 313-36, 1972.
15. ROSS, R. W. & ORLANS, E. - The redístribution of nucleic acid and the appearance of specific antigen in HeLa cells infected with herpes vírus. J. Path. Bact., 76: 393-402, 1958.
16. SASTRY, K.S. & GORDON, M.P. - The photodynamic inactivation of tobacco mosaic virus and its ribonucleic acid by acridine orange. Biochim. biophys. Acta, 129: 32-41, 1966.
17. SCHAFFER, F.L. & HACKETT, A.J. Early events ín poliovirus-HeLa cell interaction: acridíne oranre photosensitization and detergent extractíon. Virology, 21: 124-6, 1963.
18. SHORTT, H.E. & BROOKS, A.G. - Photodynamic action of methylene blue on fixed rabies vírus. Indian J. medo Res.,
21: 581-5, 1934.
19. WALLIS, C. & MELNICK, J. L. - Photodynamíc inactivation of poliovirus. Virology, 21: 332-41, 1963.
20. WALLIS, C. & MELNICK, J. L. - Irreversible photosensitization of viruses. Virology, 23: 520-7, 1964.
21. WALLIS, C.; SCHEIRIS, C. & MELNICK, J. L. - Photodynamically inactivated vaccines prepared by growing víruses in cells contaíning neutral red. J. Immun.,
99: 1134-9, 1967.
22. WILSON, J. N. & COOPER, P. D. - Aspects of the growth of poliovirus as revealed by the photodynamic effects of neutral red and acridine orange. Virology, 21: 135-45, 1963.
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
Copyright (c) 1974 Maria Irena Malachowska, Adela Roth
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