Keywords
acridine orange
effect on the virus
vírus, herpetic and vaccinic
effect on the virus
vírus, herpetic and vaccinic
How to Cite
1.
Malachowska MI, Roth A. Effect of the acridine orange dye on tierpetic and vaccinic virus in cell cultures. Rev Inst Adolfo Lutz [Internet]. 1974 Dec. 30 [cited 2024 Dec. 26];34(1-2):9-17. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/37051
Abstract
Estudou-se a ação inibidora do corante foto-sensibilizante laranja de acridina (LA) sobre os vírus herpético e vacíníco. A ação conjunta do corante e luz resultou em 100% de inibição do crescimento dos vírus, quando o LA esteve em contacto durante 1 hora com os vírus. Não se observou qualquer partícula víral nas micrografias eletrônicas, enquanto que nas células inoculadas com vírus expostos à luz, mas sem LA, observaram-se numerosas partículas de vírus. A microscopia fluorescente demonstrou diminuição de ADN em células inoculadas com mistura de LA e vírus (VLA) em comparação com os controles de células normais.
References
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17. SCHAFFER, F.L. & HACKETT, A.J. Early events ín poliovirus-HeLa cell interaction: acridíne oranre photosensitization and detergent extractíon. Virology, 21: 124-6, 1963.
18. SHORTT, H.E. & BROOKS, A.G. - Photodynamic action of methylene blue on fixed rabies vírus. Indian J. medo Res.,
21: 581-5, 1934.
19. WALLIS, C. & MELNICK, J. L. - Photodynamíc inactivation of poliovirus. Virology, 21: 332-41, 1963.
20. WALLIS, C. & MELNICK, J. L. - Irreversible photosensitization of viruses. Virology, 23: 520-7, 1964.
21. WALLIS, C.; SCHEIRIS, C. & MELNICK, J. L. - Photodynamically inactivated vaccines prepared by growing víruses in cells contaíning neutral red. J. Immun.,
99: 1134-9, 1967.
22. WILSON, J. N. & COOPER, P. D. - Aspects of the growth of poliovirus as revealed by the photodynamic effects of neutral red and acridine orange. Virology, 21: 135-45, 1963.
2. BOOTH, J.C. & STERN, H. - Phatodynamic inactivation of rubella virus. J. medo Microbiol., 5: 515-28, 1972.
3. DRAKE, J. W. & McGUIRE, J. - Propert.íes or r mutants or bacteriophage T4 photodynamically induced in the presence of thiopyranin and psoralen. J. Virol., 1: 260-7, 1967.
4. FELBER, T.D.; SMITH, E.B.; KNOX, J. M.; WALLIS, C. & MELNICK, J.L. - Photodynamic ínactívatíon of herpes simplex. J. amo medo Ass., 223: 289-92, 1973.
5. FRANKLIN, R.M. - The synthesis of fowl plague virus products in a proflavine-inhibited tis sue culture system. Virology, 6: 525-39, 1958.
6. FREIFELDER, D. & URETZ, R.B. - Mechanism of photainactivation of coliphage T7 sensitized by acridine orange. Virology, 30: 97-103, 1966.
7. HIATT, C.W.; KAUFMAN, E.; HELPRIN, J.J. & BARON, S. - Inactivation of viruses by the photodynamic action of toluidine blue. J. Immun. 845 480-4, 1960.
8. HILL JR., R.B.; BENSCH, K.G. & KING, D. W. - Photosensitization of nucleic acids and proteins. The photodynamic action of acridine orange on living cells in culture. Expl ceu Res., 21: 106-17, 1960.
9. LEI, J. C. - Der intravirale charakter der profla vinsensi bilisierten Photoinakti vierung des Maul-und-Klauenseuche-Virus. Arch .. exp. VetMed., 24: 125-39, 1970.
10. MALACHOWSKA, M. I. & 'ROTH, A. - Ação do ferro sobre o grupo dos poxvírus in vitro. Rev. Inst. Aâoljo Lutz, 31: 57-61, 1971.
11. MAYOR, H.D. - Cytochemical and fluorescent antibody studies on the growth of poliovirus in tissue culture. Tex. Rep. Biol. Med., 19: 106-22, 1961.
12. MOORE, C.; WALLIS, C. ; MELNICK, J. L. & KUNS, M. D. - Photodynamic treatment of herpes keratitis. Tnfection & Immunity, 5: 169-71, 1972,.
13. ORLOB, G.B. - Some effects of photosensitízmg dyes on three plant víruses. Virology, 21; 291-9, 1963.
14. ROMANOWSKAJA, L.N. et alii - K Woprosu o mecanizmie zwiazywania akridinowych Krasitieley z nukleinewymi kisletemi. Biofizika, 17: 313-36, 1972.
15. ROSS, R. W. & ORLANS, E. - The redístribution of nucleic acid and the appearance of specific antigen in HeLa cells infected with herpes vírus. J. Path. Bact., 76: 393-402, 1958.
16. SASTRY, K.S. & GORDON, M.P. - The photodynamic inactivation of tobacco mosaic virus and its ribonucleic acid by acridine orange. Biochim. biophys. Acta, 129: 32-41, 1966.
17. SCHAFFER, F.L. & HACKETT, A.J. Early events ín poliovirus-HeLa cell interaction: acridíne oranre photosensitization and detergent extractíon. Virology, 21: 124-6, 1963.
18. SHORTT, H.E. & BROOKS, A.G. - Photodynamic action of methylene blue on fixed rabies vírus. Indian J. medo Res.,
21: 581-5, 1934.
19. WALLIS, C. & MELNICK, J. L. - Photodynamíc inactivation of poliovirus. Virology, 21: 332-41, 1963.
20. WALLIS, C. & MELNICK, J. L. - Irreversible photosensitization of viruses. Virology, 23: 520-7, 1964.
21. WALLIS, C.; SCHEIRIS, C. & MELNICK, J. L. - Photodynamically inactivated vaccines prepared by growing víruses in cells contaíning neutral red. J. Immun.,
99: 1134-9, 1967.
22. WILSON, J. N. & COOPER, P. D. - Aspects of the growth of poliovirus as revealed by the photodynamic effects of neutral red and acridine orange. Virology, 21: 135-45, 1963.
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Copyright (c) 1974 Instituto Adolfo Lutz Journal
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