Determination of Ki-67 and CD71 antigens expression on CD34-positive blastic cells in acute leukaemias
Vol. 71 No. 1 (2012), ORIGINAL ARTICLE
Vol. 71 No. 1 (2012)

Determination of Ki-67 and CD71 antigens expression on CD34-positive blastic cells in acute leukaemias

ORIGINAL ARTICLE
https://doi.org/10.53393/rial.2012.71.32407
Published 2012-01-01
Heliana Trindade Marinho
Universidade Federal do Maranhão, Hospital Universitário Presidente Dutra, Centro de Pesquisa Clínica do Maranhão, São Luís, MA
Elda Pereira Noronha
Universidade Federal do Maranhão, Hospital Universitário Presidente Dutra, Centro de Pesquisa Clínica do Maranhão, São Luís, MA
Érika Bárbara Abreu Fonseca Thomaz
Universidade Federal do Maranhão, Departamento de Saúde Pública, São Luís, MA
Geni Lourdes de Resende Ramos Veras
Instituto Maranhense de Oncologia Aldenora Bello, São Luís, MA
Raimundo Antônio Gomes Oliveira
Universidade Federal do Maranhão, Hospital Universitário Presidente Dutra, Centro de Pesquisa Clínica do Maranhão, São Luís, MA
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Keywords

acute leukemia
cell proliferation
Ki67
CD71

How to Cite

1.
Marinho HT, Noronha EP, Thomaz Érika BAF, Veras GL de RR, Oliveira RAG. Determination of Ki-67 and CD71 antigens expression on CD34-positive blastic cells in acute leukaemias. Rev Inst Adolfo Lutz [Internet]. 2012 Jan. 1 [cited 2024 May 19];71(1):166-71. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/32407
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Abstract

The expression of Ki-67 and CD71 was determined on the immature CD34+ cells from patients with acute leukaemias (AL) and the relationship between the markers was established. 54 patients with AL were selected from December 2008 to November 2009 at Oncology Reference Center, Maranhão, Brazil. Ki-67 and CD71 expression was determined by flow cytometry in cells positive for immaturity marker CD34. Of 54 patients, 34 (63.0%) had acute lymphoid leukaemias (ALL), and 73.5% of them were type B (ALL-B) and 26.5% type T (ALL-T). The highest Ki-67 expression in bone marrow (BM) and peripheral blood (PB) was found in ALL-B than in ALL-T or acute myeloid leukaemia (AML). The highest CD71 expression was detected in BM samples from ALL-T and in PB samples from AML. An association between Ki-67 and CD71 in BM from AML and in PB from ALL-B was observed. The expression of Ki-67 in acute leukaemias did not differ in the analyzed samples; however, great differences in CD71 expression were observed in BM from ALL-T and in PB from AML. These findings will be useful for diagnosis purposes and for monitoring leukemic patients by detecting the neoplastic cell proliferation markers.
https://doi.org/10.53393/rial.2012.71.32407
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