Survival of pathogenic microorganisms in kefir
PDF

Keywords

kefir
fermented milk
inhibition
pathogens

How to Cite

1.
Dias PA, Silva DT da, Tejada TS, Leal MCGM, Conceição R de C dos S da, Timm CD. Survival of pathogenic microorganisms in kefir. Rev Inst Adolfo Lutz [Internet]. 2012 Jan. 1 [cited 2024 Dec. 4];71(1):182-6. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/32410

Abstract

Kefir is a homemade fermented milk produced by adding kefir grains. The domestic handling and the use of raw materials from different standards and sources, and the lack of inspection by qualified professionals, all this classify kefir as a food which might represent potential risks to human health. This study aimed at evaluating the pathogens survival during the kefir fermentation process. Kefir grains were added into portions of UHT skimmed milk which were experimentally contaminated with Escherichia coli O157:H7, Salmonella Typhimurium and Enteritidis, Staphylococcus aureus and Listeria monocytogenes. Analyses of the microorganism isolation in these milk samples were carried out at 0, 6, 12, 48 and 72 hours of fermentation process. Salmonella Typhimurium and Enteritidis survived for a 24-hour period in fermenting kefir. Escherichia coli O157:H7, Staphylococcus aureus and Listeria monocytogenes were recovered in less than 72 hours after the fermentation process was initiated. Under the conditions and the microorganisms concentrations established in the present study, the analyzed pathogenic bacteria survived for a period longer than those used for homemade kefir fermentation, and this one might be a potential hazard for human consumption.
https://doi.org/10.53393/rial.2012.71.32410
PDF

References

1. Chen HC, Wang SY, Chen MJ. Microbiological study of lactic acid bacteria in kefir grains by culture-dependent and culture-independent methods. Food Microbiol. 2008;25:492-501.

2. Garrote GL, Abraham AG, Antoni GL. Inhibitory power of kefir: the role of organic acids.J Food Prot. 2000;63:364-9.

3. Takizawa S, Kojima S, Tamura S, Fujinagasa S, Benno Y, Nakase T. The composition of the Lactobacillus flora in kefir grains. Syst Appl Microbiol. 1998;21:121-7.

4. Witthuhn RC, Schoema T, Britz TJ. Characterization of the microbial population at different stages of kefir production and kefir grain mass cultivation. Int Dairy J. 2005;15:383-9.

5. Farnworth ER. Kefir: a complex probiotic. Food Sci Technol Bull. Function Foods. 2005;2(1):1-17.

6. Doyle GL, Abraham AG, Antoni GL. Food microbiology: fundamentals and frontiers. 2. ed. Washington: ASM; 2001.

7. Dias PA, Conceição RCS, Coelho FJO, Tejada TS, Segatto M, Timm CD. Qualidade higiênico-sanitária de carne bovina moída e de embutidos frescais comercializados no sul do Rio Grande do Sul, Brasil. Arq Inst Biol. 2008;75:359-63.

8. Conceição RCS, Hentges A, Moreira AN, Vasconcellos FA, Ângelo IMR, Carvalhal JB, et al. Isolamento de Salmonella de produtos de frango e perfil de suscetibilidade dos isolados a antimicrobianos. Rev Inst Adolfo Lutz. 2007;66:31-4.

9. U. S. Food and Drug Administration – FDA. Center for Food Safety and Applied Nutrition. Staphylococcus aureus. In: Foodborne pathogenic microorganisms and natural toxins handbook. [Accessed 2010 May 31]. Available in: [http://www.cfsan.fda.gov/~mow/chap3.html].

10. Andrews WH, Hammack T. Salmonella. U.S. Food and Drug Administration, Bacteriological analytical manual online, chapter 5, 2007. [Accessed 2010 May 31]. Available in: [http://www.cfsan.fda.gov/~ebam/bam-5.html].

11. Hitchins AD. Detection and enumeration of Listeria monocytogenes in foods. U.S. Food and Drug Administration, Bacteriological analytical manual online, Chapter 10, 2003. [Accessed 2010 May 28]. Available in: [http://www.cfsan.fda.gov/~ebam/bam-10.html].

12. Bennett RW, Lancette GA. Staphylococcus aureus. U.S. Food and Drug Administration, Bacteriological analytical manual online, chapter 12, 2001. [Accessed 2010 May 31]. Available in: [http://www.cfsan.fda.gov/~ebam/bam-12.html].

13. Gulmez M, Guven A. Behaviour of Escherichia coli O157:H7, Listeria monocytogenes 4b and Yersinia enterocolitica O3 in pasteurized and non-pasteurised kefir fermented for one or two days. Int J Food Sci Tech. 2003;9:365-9.

14. Kasimoglu A, Akgun S. Survival of Escherichia coli O157:H7 in the processing and post-processing stages of acidophilus yogurt. Int J Food Sci Tech. 2004;39:563-8.

15. Czamansky RT. Avaliação da atividade antimicrobiana de filtrados de quefir artesanal. [dissertação de mestrado em medicina veterinária preventiva]. Porto Alegre (RS): Universidade Federal do Rio Grande do Sul; 2003.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright (c) 2012 Instituto Adolfo Lutz Journal

Downloads

Download data is not yet available.