Culture media made by bovine whey for Staphylococcus aureus growth
Vol. 71 No. 1 (2012), SCIENTIFIC NOTE
Vol. 71 No. 1 (2012)

Culture media made by bovine whey for Staphylococcus aureus growth

SCIENTIFIC NOTE
https://doi.org/10.53393/rial.2012.71.32413
Published 2012-01-01
Renata Albuquerque Costa
Universidade Federal do Ceará, Instituto de Ciências do Mar, Laboratório de Microbiologia Ambiental e do Pescado, Fortaleza, CE
Márcia Viviane de Brito
Universidade Estadual Vale do Acaraú, Departamento de Biologia, Sobral, CE
Verônica Sousa de Brito
Universidade Estadual Vale do Acaraú, Departamento de Biologia, Sobral, CE
Joseíres Lira de Sousa Fontenelle
Universidade Estadual Vale do Acaraú, Departamento de Biologia, Sobral, CE
Jackson Rafael Oliveira Peixoto
Universidade Federal do Ceará, Laboratório de Microbiologia Ambiental e do Pescado, Fortaleza, CE
Gustavo Hitzschky Fernandes Vieira
Universidade Estadual Vale do Acaraú, Departamento de Biologia, Sobral, CE
Regine Helena Silva dos Fernandes Vieira
Universidade Federal do Ceará, Instituto de Ciências do Mar, Fortaleza, CE
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Keywords

staphylococcus aureus
bovine whey
experimental media

How to Cite

1.
Costa RA, Brito MV de, Brito VS de, Fontenelle JL de S, Peixoto JRO, Vieira GHF, Vieira RHS dos F. Culture media made by bovine whey for Staphylococcus aureus growth. Rev Inst Adolfo Lutz [Internet]. 2012 Jan. 1 [cited 2024 Dec. 4];71(1):198-201. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/32413
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Abstract

We investigated the cultivation of Staphylococcus aureus ATCC 25923 in experimental media containing bovine whey. Liquid (whey broth – WB) and solid (whey agar – WA) media supplemented with sodium chloride (WB+NaCl and WA+NaCl) and yeast extract (WB+yeast and WA+yeast) were prepared. In WA media, the enrichment with yeast extract (0.1%) and water dilution (40%) (WA+H2O+yeast) was tested. Commercial brain heart infusion broth (BHI) and Baird-Parker agar (BP) media were used as controls. The efficiency of experimental culture media was assessed by means of biomass determination and colony counts by using S. aureus ATCC 25923 strain. The bacterial growths in all experimental media were identical to those found in control media. In addition, grown bacteria colonies showed morphological characteristics compatible with ATCC S. aureus, especially in the WA+H2O+yeast medium. Therefore, the bovine whey proved to be efficient as an alternative culture medium for S. aureus isolation and growth.
https://doi.org/10.53393/rial.2012.71.32413
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