Keywords
bacterial inhibition
bacterial inactivation
antioxidants
anthocyanin
How to Cite
Abstract
The hibiscus (Hibiscus sabdariffa L.) has antioxidant and antimicrobial properties, it can be used as functional food and medicinal plant. This study aimed at determining the intensity of activity inhibition (IINIB) and of inactivation (IINAB) of two alcoholic extracts obtained from calyxes and fruits with seeds of different accesses of hibiscus on international standards bacterial Enterococcus faecalis, Escherichia coli, Salmonella Enteritidis and Staphylococcus aureus. And the correlation between these results and the presence of total polyphenols and anthocyanins was done. The antimicrobial activity of alcoholic extract from both accesses showed a significant difference. While Salmonella Enteritidis (11,5) and Escherichia coli (12) were more susceptible to the alcoholic extract from hibiscus calyxes and fruits with seeds, respectively, the Staphylococcus aureus (5.2 e 0.1) was the most resistant in both extracts. The IINIB values were higher than IINAB values, showing that the bacteriostatic activity was usually higher than the bactericide. Total polyphenols and anthocyanins contents in calyxes alcoholic extract showed a significant difference, and they were higher than in alcohol extract from fruits with seeds. It seems likely that occurs a direct correlation between the anthocyanin concentration and the antibacterial activity in different structures of the hibiscus plant.References
1. Forsythe, SJ. Microbiologia da Segurança Alimentar. Porto Alegre (RS): Artmed; 2002. 424 p.
2. Pereira MC, Vilela GR, Costa LMAS, Silva RF, Fernandes AF, Fonseca EWN, et al. Inibição do desenvolvimento fúngico através da utilização de óleos essenciais de condimentos. Ciênc Agrotec. 2006;30(4):731-8.
3. Souza EL, Lima EO, Narain N. Especiarias: uma alternativa para o controle da qualidade sanitária e de vida útil de alimentos, frente às novas perspectivas da indústria alimentícia. Hig Aliment. 2003;17(113):38-42.
4. Sartoratto A, Machado ALM, Delarmelina C, Figueira GM, Duarte, MCT, Rehder VLG, et al. Composition and antimicrobial activity of essential oils aromatic plants used in Brazil. Braz J Microbiol. 2004;35:275-80.
5. Peng Y, Yuan J, Liu F, Ye J. Determination of active components in rosemary by capillary electrophoresis with electrochemical detection. J Pharm Biomed Anal. 2005;39(3-4):431-7.
6. Lorenzi H, Matos FJA. Plantas medicinais no Brasil nativas e exóticas. São Paulo (SP): Instituto Plantarum de Estudos da Flora; 2002.
7. Martins ER, Castro DM, Castellano DC, Dias JE. Plantas medicinais. Viçosa (MG): Universidade Federal de Viçosa; 1994.
8. Panizza S. Plantas que curam: cheiro de mato. 2. ed. São Paulo (SP): IBRASA; 1997.
9. McCaleb RS. Roselle Production Manual (Hibiscus sabdariffa). Herb Research Foundation, USA, 1998. [acesso 2009 dez 20]. Disponível em: [http://www.herbs.org/.africa/hibiscus production manual.html].
10. Castro NEA, Pinto JEBP, Cardoso MG, Morais AR, Bertolucci SKV, Silva FG, et al. Planting time for maximization of yield of vinegar plant calyx (Hibiscus sabdariffa L.). Ciênc Agrotec. 2004;28(3):542-51.
11. Mahadevan N, Shivali, Pradeep K. Hibiscus sabdariffa Linn. An overview. Nat Prod Radiance. 2009;8(1):77-83.
12. Liu KS, Tsao SM, Yin MC. In vitro antibacterial activity of roselle calyx and protocatechuic acid. Phytother Res. 2005; 19:942-5.
13. D’Heurex-Calix F, Badrie N. Consumer acceptance and physicochemical quality of processed red sorrel/roselle (Hibiscus sabdariffa L.) sauces from enzymatic extracted calyces. Food Serv Technol. 2004;4:141-8.
14. Wang CJ, Wang JM, Lin WL, Chu CY, Chou FP, Tseng TH. Protective effect of hibiscus anthocyanins against tert-butyl hydroperoxide-induced hepatic toxicity in rats. Food Chem Toxicol. 2000;38(5):411-16.
15. Brasil. Ministério da Saúde. Portaria nº 519, de 26 de junho de 1998. Aprova o Regulamento Técnico para Fixação de Identidade e Qualidade de “Chás – Plantas Destinadas à Preparação de Infusões ou Decocções”. Diário Oficial [da] República Federativa do Brasil. Brasília, DF, 29 jun 1998.
16. Vilche C, Gely M, Santalla E. Physical properties of quinoa seeds. Biosyst Eng. 2003;86(1):59-65.
17. Morton JF. Roselle. In: Morton JF. Fruits of warm climates. Miami, FL: Florida Fair Books, 1987, p. 281-6.
18. Chao CY, Yin MC. Antibacterial effects of Roselle Calyx extracts and protocatechuic acid in ground beef and apple juice. Foodborn Pathog Dis. 2009;6(2):201-6.
19. Akindahunsi AA, Olaleye MT. Toxicological investigation of aqueous–methanolic extract of the calyces of Hibiscus sabdariffaL. J Ethnopharmacol. 2003;89:161-4.
20. Avancini CAM, Wiest JM. Atividade desinfectante do decocto de Hypericum caprifoliatum Cham. Eshclecht. Guttiferae (“escadinha/sinapismo”) frente a diferentes doses infectantes de Staphylococcus aureus (agente infeccioso em mastite bovina). Rev Bras Plantas Med. 2008;10(1):64-9.
21. Romeiro RS. Técnica de microgota para contagem de células bacterianas viáveis em uma suspensão. Laboratório de Bacteriologia de Plantas. Viçosa (MG): Universidade Federal de Viçosa. [acesso 2009 abr 05]. Disponível em: [http://www.ufv.br/dfp/bac/uni9.pdf ].
22. Cavalli-Sforza L. Biometrie: Grundzüge biologisch-medizinische Statistic (Biometria: fundamentos de estatística viológica-médica). Stuttgart: Gustav Fisher, 1974. p. 201-4.
23. Deutsche Veterinärmedizinische Gesellschaft (DVG)/ Sociedade Alemã de Medicina Veterinária. Richtlinien zur Prüfung chemischer Desinfektionsmittel für die Veterinärmedizin/ Normas para a testagem de desinfetantes químicos para a medicina veterinária. Giessen, 1980. In: Schliesser Th, Strauch D. Desinfektion in Tierhaltung, Fleisch- und Milschwirtschaft/Desinfecção na produção animal, em laticínios e em frigoríficos. Stuttgart: Enke Verlag, 1981.
24. Callegari-Jacques SM. Bioestatística: princípios e aplicações. Porto Alegre (RS): Artmed; 2003.
25.Vinson JA, Su X, Zubik L, Bose P. Phenol antioxidant quantity and quality in foods: fruits. J Agric Food Chem. 2001;49(11):5315-21.
26. Karou D, Dicko MH, Simpore J, Traore AS. Antioxidant and antibacterial activities of polyphenols from ethnomedicinal plants of Burkina Faso. Afr J Biotechnol. 2005;4(8):823-8.
27. Kuskoski EM, Asuero AG, Morales MT, Fett R. Frutas Tropicais Silvestres e Polpas de Frutas Congeladas: Atividade Antioxidante, Polifenóis e Antocianinas. Ciênc Rural. 2006;36(4):1283-7.
28.Teixeira L, Stringheta PC, Oliveira FA. Comparação de métodos para quantificação de antocianinas. Rev Ceres. 2008;4(55):297-304.
29. Carvalho HHC, Cruz FT, Wiest JM. Atividade antibacteriana em plantas com indicativo etnográfico condimentar em Porto Alegre, RS/Brasil. Rev Bras Plantas Med. 2005;7(3):25-32.
30. Gobbo-Neto L, Lopes NP. Plantas medicinais: fatores de influência no conteúdo de metabólitos secundários. Quim Nova. 2007;30(2):374-81.
31. Olaleye MT. Cytotoxicity and antibacterial activity of Methanolic extract of Hibiscus sabdariffa. J Med Plant Res. 2007;1(1):9-13.
32. Juliani HR, Welch CR, Wu Q, Diouf B, Malainy D, Simon JE. Chemistry and Quality of Hibiscus (Hibiscus sabdariffa) for Developing the Natural-Product Industry in Senegal. J Food Sci. 2009;74(2):113-21.
33. Sáyago-Ayerdi SG, Arranz S, Serrano J, Goñi I. Dietary fiber content and associated antioxidant compounds in Roselle flower (Hibiscus sabdariffa L.) beverage. J Agric Food Chem. 2007;55(19):7886-90.
34. Tsai PJ, McIntosh J, Pearce P, Camden B, Jordan BR. Anthocyanin and antioxidant capacity in Roselle (Hibiscus Sabdariffa L.) extract. Food Res Int. 2002;35:351–6.
35. Fullerton M, Khatiwada J, Johnson JU, Davis S, Williams LL. Determination of antimicrobial activity of sorrel (Hibiscus sabdariffa) on Esherichia coli O157:H7 isolated from food, veterinary, and clinical samples. J Med Food. 2011;14(9):950-6.
36. Yang L, Gou Y, Zhao T, Zhao J, Li F, Zhang B, et al. Antioxidant capacity of extracts from calyx fruits of roselle (Hibiscus sabdariffa L.). Afr J Biotechnol. 2012;11(17): 4063-8.
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