Occurrence of Cronobacter spp. (Enterobacter sakazakii) in infant food purchased in a public hospital
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Keywords

Cronobacter spp. (E. sakazakii)
infant formulas
neonates

How to Cite

1.
Freitas LG, Ristori CA, Jakabi M, Paula AMR de, Rowlands REG. Occurrence of Cronobacter spp. (Enterobacter sakazakii) in infant food purchased in a public hospital. Rev Inst Adolfo Lutz [Internet]. 2011 Apr. 1 [cited 2024 May 16];70(4):548-53. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/32513

Abstract

Cronobater spp. (E. sakazakii) is considered an opportunistic pathogen and the public health authorities have given attention to this matter because of the increasing number of infection outbreaks in neonates and infants. The bacterium is associated with rare cases which can cause meningitis, necrotizing enterocolitis and septicemia, with a high mortality rate. Cronobacter spp. is widely disseminated, but only the powdered infant milk formulas were epidemiologically linked to diseases caused by this agent. The aim of this study was to evaluate the occurrence of Cronobacter spp. in foods for children aged 0-36 months, that were purchased in a public hospital in São Paulo. Twenty six reconstituted samples and 24 powdered formulas were analyzed according to the methodology established by ISO. Cronobacter spp. was detected in one sample (3.8%) of reconstituted infant food based on corn flour and in other four samples (16.7%) of these powered products . The bacterium was not detected in infant formula for children aged 0-6 months, although its presence in baby food might contribute to contaminate the lactary environment and devices by cross-contamination.

https://doi.org/10.53393/rial.2011.v70.32513
PDF (Português (Brasil))

References

1. Food and Agriculture Organization/World Health Organization - FAO/WHO. Enterobacter sakazakii (Cronobacterspp.) in powdered follow-up formulae. Microbiological Risk Assessment Series, 2008. [acesso 2008 Jun 15]. Disponível em: [http://www.who.int/foodsafety/publications/micro/mra_followup/en/index.html].

2. World Health Organization - WHO. Enterobacter sakazakii and other microorganisms in powdered infant formula. In: Joint FAO/ WHO Workshop; 2004; Geneva, Sz. [Acesso 2008 Jun 20]. Disponível em [http://www.who.int/foodsafety/micro/jemra/meetings/feb2004/en/].

3. Healy B, Cooney S, O ́Brien S, Iversen C, Whyte P, Nally J, et al. Cronobacter (Enterobacter sakazakii): An Opportunistic Foodborne Pathogen. Foodborne Pathog Dis. 2010;7(4):339-50.

4. Food and Drug Administration - FDA. Health professionals letter on Enterobacter sakazakii infections associated with used of powdered (dry) infant formulas in neonatal intensive care units. 2002. [acesso 2007 Jun 20]. Disponível em: [http://www.fda.gov/Food/FoodSafety/Product-SpecificInformation/InfantFormula/AlertsSafetyInformation/ucm111299.htm].

5. Van Acker J de, Smet F, Muyldermans G, Bougatef A, Naessens A, Lauwers S. Outbreak of necrotizing enterocolitis associated with Cronobacter spp. in powdered milk formula. J Clin Microbiol. 2001;39(1):293-7.

6. Centers for Diseases Control and Prevention - CDC. Enterobacter sakazakii infections associated with the use of powdered infant formula-Tennessee. MMWR Morb Mortal Wkly Rep. 2002;51(14):297-300.

7. Barreira ER, Souza DC, Góis PF, Fernandes JC. Meningite por Enterobacter sakazakii em recém-nascido: relato de um caso. Pediat. 2003;25(1/2):65-70.

8. Lai KK. Enterobacter sakazakii infection among neonates, infants, children, and adults: case report and review of the literature. Medicine. 2001;80(2):113-22.

9. Farber JM. E. sakazakii: new foods for thought? Lancet. 2004;363(9402):5-6.

10. World Health Organization - WHO. Enterobacter sakazakii and other microorganisms in powdered infant formulas: meeting report. In: Microbiological Risk Assessment Series. 2007. [Acesso 2008 Jun 20]. Disponível em: [http://www.who.int/foodsafety/publications/micro/mra/index/html/].

11. Kandhai MC, Reij MW, Van-Puyvelde K, Guillaume-Gentil O, Beumer, RR, Van Schothorst M et al. Occurrence of E. sakazakii in food production environments and households. Lancet. 2004;363(9402):39-40.

12. Hamilton JV, Lehane MJ, Braig HR. Isolation of Enterobacter sakazakii from midgut of Stomoxys calcitrans. Emerg Infect Dis. 2003;9(10):1355-6.

13. Mullane NR, Iversen C, Healy B, Walsh C, Whyte P, Wall PG, et al. Enterobacter sakazakii an emerging bacterial pathogen with implications for infant health. Minerva Pediatr. 2008; 59(2):137-48.

14. Leclercq A, Wanegue C, Baylac P. Comparasion of fecal coliform agar and violet red bile lactose agar for fecal coliform enumeration in foods. Appl Environ Microbiol. 2002;68(4):1631-8.

15. Friedemann M. Enterobacter sakazakii in food and beverages (other than infant formula and milk powder). Int J Food Microbiol. 2007;116(1):1-10.

16. Iversen C, Forsythe SJ. Comparison of Media for the Isolation of Enterobacter sakazakii. Appl Environ Microbiol. 2007;73(1):48-52.

17. Lehner A, Stephan R. Microbiological, epidemiological, and food safety aspects of Enterobacter sakazakii. J Food Prot. 2004;67(12):2850-7.

18. Gillio CM. Enterobacter sakazakii em fórmulas lácteas infantis desidratadas para bebês de 0-6 meses [dissertação de mestrado]. São Paulo (SP): Universidade de São Paulo; 2006.

19. Palcich G. Ocorrência de Enterobacter sakazakii no ambiente de lactários de Maternidades da Grande São Paulo [dissertação de mestrado]. São Paulo (SP): Universidade de São Paulo; 2007.

20. Santos, RFS. Ocorrência de Enterobacter sakazakii em fórmulas infantis para lactantes em Hospitais e Maternidades da região de Campinas – SP [dissertação de mestrado]. Campinas (SP): Universidade Estadual de Campinas; 2006.

21. Santos M, Silva C, Sampaio J, Marangoni D, Pinto M, Moreira B. Detection and control of Enterobacter sakazakii sepsis outbreak in four hospitals in Rio de Janeiro, Brazil. Infect Control Hosp Ep. 2000;21(2):140.

22. Nero LA. Listeria monocytogenes e Salmonella spp. em leite cru produzido em quatro regiões leiteiras no Brasil: ocorrência e fatores que interferem na sua detecção [tese de doutorado]. São Paulo (SP): Universidade de São Paulo; 2005.

23. International Standard Organization (ISO) TC34/SC 5N. Milk and Milk products – Detection of Enterobacter sakazakii. Geneva; 2004.

24. Bar-Oz B, Peleg O, Block C, Arad, I. Enterobacter sakazakiiinfection in the newborn. Acta Pediatri. 2001;90(3):356-8.

25. Block C, Peleg O, Bar-Oz B, Simhon A, Arad I, Shapiro M et al. Cluster of neonatal infections in Jerusalem due to unusual biochemical variant of Enterobacter sakazakii. Eur J Clin Microbiol Infect Dis. 2002;21(8):613-6.

26. Simmons BP, Gelfand MS, Haas M, Metts L, Ferguson J. Enterobacter sakazakii infections in neonates associated with intrinsic contamination of a powdered infant formula. Infect Control Hosp Ep. 1989;10 (9):398-401.

27. Restaino L, Frampton EW, Lionberg WC. Becker RJ. A chromogeninc plating medium for the isolation and identification of Enterobacter sakazakii from foods, food ingredients, and environmental soucers. J Food Prot. 2006;69(2):315-22.

28. Shaker R, Osaili T, Al-Omary W, Jaradat, Z, Al-Zuby, M. Isolation of Enterobacter sakazakii and other Enterobacter sp. from food and food production environments. Food Control. 2007;18(7): 1241-5.

29. Nazarowec-White M, Farber JM. Incidence, survival and growth of Enterobacter sakazakii in infant formula. J Food Prot.1997;60(3):103-13.

30. Iversen C, Forsythe, SJ. Risk profile of Enterobacter sakazakii, an emergent pathogen associated with infant milk formula. Trends Food Sci Tech. 2003;14(11): 443-54.

31. Nazarowec-White M, Farber JM. Thermal resistence of Enterobacter sakazakii in reconstituted dried-infant formula. Lett Appl Microbiol .1997;24(1):9-13.

32. Breeuwer P, Lardeau A, Peterz M, Joosten HM. Desiccation and heat tolerance of Enterobacter sakazakii. J Appl Microbiol. 2003;95(5):967-73.

33. Barron JC, Forsythe S. Dry stress and survival time of Enterobacter sakazakii and other Enterobacteriaceae in dehydrated powdered infant formula. Food Protect. 2007;70(9):2111-7.

34. Gurtler JB, Beuchat LR. Growth of Enterobacter sakazakii in reconstituted infant formula as affected by composition and temperature. J Food Prot. 2007;70(9):2095-103.

35. Osaili T, Shaker R, Ayyash M, Al-Nabulsi AA, Forsythe SJ. Survival and growth of Cronobacter species (Enterobacter sakazakii) in wheat-based infant follow-on formulas. Lett Appl Microbiol. 2008;48(4):408-12.

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