Keywords
cell line
cellular characterization
cross contamination
How to Cite
Abstract
The evidence of the species origin has been a crucial characteristic for cell line certification, which can be performed by isoenzymes electrophoresis. Aiming at standardizing the isoenzyme electrophoresis technique at the Cell Culture Nucleus of IAL, cell extracts from different animal species were investigated on horizontal or vertical electrophoresis using polyacrylamide or agarose gels, 100 V at 4oC. The glucose-6- phosphate dehydrogenase staining was carried out using tetrazolium salts. A single band for each tested cell line was detected, with the expected migration pattern for the different animal species used, excepting the bovine cell line MDBK which did not show one of the bands, probably due to low enzyme expression. After analyzing the results from different electrophoretic systems and gels testing, the horizontal electrophoresis with 2% agarose gels showed the best performance. This technique standardization and implementation will turn the laboratory be feasible in providing cell line with high quality control.
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