Keywords
bovine mastitis
mecA
Staphylococcus spp
How to Cite
Abstract
Mastitis is of a major concern among the diseases that affect the dairy herd due to the economic problems and public health concerns. Staphylococcus spp has been the infectious bacterium and the etiologic agent of this disease. The oxacillin resistance of Staphylococcus spp isolated from milk samples of animals with a history of recurrent mastitis was investigated. This study was performed by means of gel diffusion test, agar screening, minimum inhibitory concentration (MIC), and the mecA gene searching. Of 134 milk samples, Staphylococcus spp was isolated from 55.22% samples (74/134). By means of disk diffusion test, the majority of the isolates showed multiresistance, and 51.35% (38/74) were resistant to oxacillin. This profile was confirmed in parallel by CMI and the agar screening. By molecular analysis 33.78% of isolates (25/74) revealed the mecA gene, being frequently isolated from Staphylococcus aureus and coagulase negative Staphylococcus at rates of 48% (12/25) and 32% (8/25 ), respectively, and followed by S. intermedius in 16% (4 / 25) and S. hyicus in 4% (1/25). The serious problem associated with oxacillin-resistant Staphylococcus aureus was found this study, including in rural environment and these findings justifie the treatment difficulty and the recurrence of infection.References
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