Keywords
Melaleuca alternifolia
antifungal activity
essencial oil
How to Cite
Abstract
Oral candidiasis is a fungal infection mostly common in people infected with HIV and the recurrent episodes occur in patients with AIDS. Aiming at searching, a new and efficacious drug against resistant strains, the antifungal activity of essential oil from Melaleuca alternifolia at different concentrations was assessed on yeasts isolated from oral candidiasis. The experiment was performed by using agar diffusion
technique; and the antifungal effect was evaluated on the standard strains of Candida albicans ATCC 10231, Candida tropicalis ATCC 157, Candida glabrata ATCC 30070, Candida krusei ATCC 6258 and Candida dubliniensis ATCC 778 157, and on the isolated from the oral cavity of HIV positive pregnant women,
being seven C. albicans, one C. tropicalis, one C glabrata and one C. krusei. The essential oil was analyzed in quantities of 20 and 50 μL at 10-100% concentrations, ranging from 10 to 10%. All of the tested strains were susceptible to the M. alternifolia essential oil at concentrations of 70% and 50% in volumes of 20 μL and 50 μL, respectively. The antifungal activity of essential oil from M. alternifolia holds one´s attention in manufacturing new and effective drugs.
References
1. Souza LB, Pinto LP, Medeiros AMC, Araújo Junior RF, Mesquita OJX. Manifestações orais em pacientes com AIDS em uma população brasileira. Pesqui Odontol Bras. 2000; 14 (1): 79-85.
2. Shmidt-Westhausen AM, Bendick C, Reichart PA, Samaranayake LP. Oral candidosis and associated Candida species in HIV- infected Cambodians exposed to antimytotics. Mycoses. 2004; 47: 435-1.
3. Wingeter MA, Guilhermetti E, Shinobre CS, Takaki I, Svidizinski IE. Identificação microbiológica e sensibilidade in vitro de Candida isoladas da cavidade oral de indivíduos HIV positivos. Rev Soc Bras Med Trop. 2007; 40 (3): 272-6.
4. Sant´Ana PL, Milan EP, Martinez R, Queiroz-Telles F, Ferreira MS, Alcântara AP et al. Multicenter brazilian study of oral Candida species isolated from aids patients. Mem Inst Oswaldo Cruz. 2002; 97 (2): 253-7.
5. Chavasco JK, Paula CR, Hirata MH, Aleva NA, Melo CE, Gambale W et al. Molecular identification of Candida dubliniensis isolated from oral lesions of HIV-positive and HIV-negative patients in São Paulo, Brazil. Rev Inst Med Trop São Paulo. 2006; 48 (1): 21-6.
6. Mariano PLS, Milan EP, Matta DA, Colombo AA. Candida dubliniensis identification in brazilian yeast stock collection. Mem Ins Oswaldo Cruz. 2003; 98 (4): 533-8.
7. Cox SD, Mann CM, Markham JL, Gustafson JE, Warmington JR, Wyllie SG. Determining the antimicrobial actions of tea tree oil. Molecules. 2001; 6: 87-91.
8. Cox SD, Mann CM, Markham JL, Bell HC, Gustafson JE, Warmington JR et al. The mode of antimicrobial action of the essential oil of Melaleuca alternifolia (tea tree oil). J Appl Microbiol. 2000; 88: 170-5.
9. Hammer KA, Carson CF, Riley TV. Antifungal effects of Melaleuca alternifolia (tea tree) oil and its components on Candida albicans, Candida glabrata and Saccharomyces cerevisiae. J Antimicrob Chemother. 2004; 53: 1081-5.
10. Silva CJ, Barbosa LCA, Maltha CRA, Pinheiro AL, Ismail FMD. Comparative study of the essential oils of seven Melaleuca (Myrtaceae) species grown in Brazil. Flavour Frag J. 2007; 22: 474-8.
11. Costa ACBP, Pereira CA, Freire F, Junqueira JC, Jorge AOC. Atividade antifúngica dos extratos glicólicos de Rosmarinus officinalis Linn. e Syzygium cumini Linn. sobre cepas clínicas de Candida albicans, Candida glabrata e Candida tropicalis. Rev Odontol UNESP. 2009; 38 (2): 111-6.
12. Mondello F, Bernardis F, Girolamo A, Salvatore G, Cassoni A. In vitro and in vivo activity of tea tree oil against azole-susceptible and- resistant human pathogenic yeasts. J Antimicrob Chemother. 2003; 51: 1223-9.
13. Hammer KA, Carson CF, Riley TV. In vitro activity of essential oils, in particular Melaleuca alternifolia (tea tree) oil and tea tree oil products, against Candida spp. J Antimicrob Chemother. 1998; 42: 591-5.
14. Vazquez JA, Arganoza MT, Boikov D, Vaishampayan JK, Akins RA. In vitro susceptibilities of Candida and Aspergillus species to Melaleuca alternifolia (tea tree) oil. Rev Iberoam Micol. 2000; 17: 60-3.
15. Silva JO, Franceschinf AS, Candido RC. Presença de leveduras em mucosas e fezes de indivíduos aparentemente saudáveis e de pessoas com sintomas de infecção fúngica. Rev Ins Adolfo Lutz. 2002; 61(2): 113-20.
16. Jandourek A, Vaishampayan JK, Vazquez JA. Efficacy of melaleuca oral solution for the treatment of fluconazole refractory oral candidiasis in AIDS patients. AIDS. 1998; 12: 1022-37.
17. Catalán A, Pacheco JG, Martinéz A, Mondaca MA. In vitro and in vivo activity of Melaleuca alternifolia mixed with tissue conditioner on Candida albicans. Surg Oral Med Oral Pathol Oral Radiol Endod. 2008; 105: 327-32.
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