Keywords
microbiological analysis
in vivo and in vitro toxicity
How to Cite
Abstract
Papers manufactured for sanitary purposes are absorbing products, composed by natural fibers, and used to produce hygienic paper, handkerchiefs, towels, napkins, and hospital sheets. Sixty-five products from different manufacturers and marketed in São Paulo, Brazil were investigated by in vivo and in vitro dermal toxicity assays and microbiological quality analysis. The assays were performed following ABNT NBR 15134 Regulation and Ministerial Decree 1480. In vivo assay was carried out by Draize and Magnusson and Kligman techniques, and in vitro testing was performed by agar diffusion NCTC cell strains clone 929 (ATCC-CCL1). Microbiological analysis was performed according to Ministerial Decree 1480. The analyzed products showed satisfactory results on both in vivo and in vitro assays. Forty-nine (75.4%) samples showed satisfactory microbiological quality, but unsatisfactory in 16 (24.6%) samples due to the occurrence of aerobic mesophilic bacteria and Clostridium spp. In vitro ytotoxicity assay could be used as screening test, and as an alternative test for replacing the animal-using methodologies. The microbiological analysis is not included in the pertinent guidelines, but the use of analytical marker is crucial for assessing the sanitary-hygienic
conditions and also to act in compliance with Good Manufacturing and Control Practices.
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