Implementation and optimization of real time PCR for diagnosis of pandemic influenza A (H1N1) at Instituto Adolfo Lutz and perspectives for 2010
Vol. 69 No. 1 (2010), SCIENTIFIC NOTE
Vol. 69 No. 1 (2010)

Implementation and optimization of real time PCR for diagnosis of pandemic influenza A (H1N1) at Instituto Adolfo Lutz and perspectives for 2010

SCIENTIFIC NOTE
https://doi.org/10.53393/rial.2010.69.32686
Published 2010-01-01
Lucila Okuyama Fukasawa
Instituto Adolfo Lutz, Centro de Imunologia, São Paulo, SP
Daniela Bernardes Silva
Instituto Adolfo Lutz, Centro de Virologia, São Paulo, SP
https://orcid.org/0000-0002-5686-7979
Fabiana Cristina Pereira dos Santos
Instituto Adolfo Lutz, Centro de Virologia, São Paulo, SP
Isabel Takano Oba
Instituto Adolfo Lutz, Centro de Virologia, São Paulo, SP
Adele Caterino-de-Araújo
Secretaria da Saúde de São Paulo, Coordenadoria de Controle de Doenças, Instituto Adolfo Lutz, Centro de Imunologia, São Paulo, SP
Maria Gisele Gonçalves
Instituto Adolfo Lutz, Centro de Imunologia, São Paulo, SP
Fábio Higa
Instituto Adolfo Lutz, Centro de Imunologia, São Paulo, SP
Maristela Marques Salgado
Instituto Adolfo Lutz, Centro de Imunologia, São Paulo, SP
Claudio Tavares Sacchi
Instituto Adolfo Lutz, Centro de Imunologia, São Paulo, SP
Grupo de Trabalho de Influenza A (H1N1)
Instituto Adolfo Lutz
PDF (Português (Brasil))

Keywords

influenza A (H1N1)
molecular diagnosis
real time PCR (rRT-PCR)
algorithms of molecular assays

How to Cite

1.
Fukasawa LO, Silva DB, Santos FCP dos, Oba IT, Caterino-de-Araújo A, Gonçalves MG, Higa F, Salgado MM, Sacchi CT, de Influenza A (H1N1) G de T. Implementation and optimization of real time PCR for diagnosis of pandemic influenza A (H1N1) at Instituto Adolfo Lutz and perspectives for 2010. Rev Inst Adolfo Lutz [Internet]. 2010 Jan. 1 [cited 2024 Dec. 4];69(1):131-5. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/32686
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Abstract

The authors present their experience with the molecular diagnosis of Influenza A (H1N1) with 37.240 clinical samples obtained from individuals suspected of flu, sent to Instituto Adolfo Lutz for analysis. They show the used algorithms, compare their efficiency in terms of sensitivity, specificity and cost, and suggest a new algorithm to be employed in case of an outbreak of Influenza A (H1N1) in 2010.
https://doi.org/10.53393/rial.2010.69.32686
PDF (Português (Brasil))

References

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Copyright (c) 2010 Instituto Adolfo Lutz Journal

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