Assessment of physical-chemical characteristics and hygienic and sanitary conditions indicators in water supplied at public primary schools
PDF (Português (Brasil))

Keywords

Pseudomonas aeruginosa
schools
potable water
water supply

How to Cite

1.
Almeida VF da S, Oliveira SR de, Jácome PRL de A, Jácome-Júnior AT. Assessment of physical-chemical characteristics and hygienic and sanitary conditions indicators in water supplied at public primary schools. Rev Inst Adolfo Lutz [Internet]. 2009 Aug. 1 [cited 2024 Dec. 4];68(3):334-40. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/32691

Abstract

This study analyzed the hygienic and sanitary conditions of the water samples used for drinking and for preparing scholar foods at public schools located in Caruaru city, Pernambuco, Brazil, by searching the occurrence of Pseudomonas aeruginosa and coliform group. Tap water samples were collected from the kitchens of 36 public schools located in Caruaru city, in which the people rates including students, teachers and school staff were more than 100, and teaching children aged from 0 to 5 years. The analyzed samples showed pH on average of 7.04 ± 0.53 at room temperature. Pseudomonas aeruginosa was detected in 83.3% of samples and coliforms were found in 69.4%; and 13.9% of water samples were negative for both bacteria. Among 20% of Pseudomonas aeruginosa positive samples, no coliform was found; 61.1% of Pseudomonas aeruginosa positive samples were directly related to the lack of adequate water reservoir cleansing. These findings showed that Pseudomonas aeruginosa investigation should be performed as a standard sanitary indicator for assessing drinking water condition, as according to the decree 518 of March 25, 2004 the water sample contaminated with Pseudomonas aeruginosa has not been the reason for disapproving it. This procedure is crucial because adding to the fact of Pseudomonas aeruginosa being an opportunistic pathogen, it is considered an indicator of pollution with organic material in water stored in reservoirs, which could be potential source of pathogens proliferation.

https://doi.org/10.53393/rial.2009.v68.32691
PDF (Português (Brasil))

References

1. Brasil. Ministério da Agricultura, Pecuária e Abastecimento Decreto nº 30.691, de 29 de março de 1952. Aprova o Regulamento de Inspeção Industrial e Sanitária de Produtos de Origem Animal. Diário Oficial [da] União, Brasília,DF, 2007; p.10785, 07 de jul de 1952 Seção 1.

2. Constituição Federal. Lei 11.101, de 9 fevereiro de 2005. Capítulo III Dos crimes contra saúde pública, art. 272. Código Penal. Código de Processo Penal Organização Luiz Flávio Gomes, 9 Ed. Ver., ampl., e atual. SP. Editora Revista dos Tribunais, RT minicódigos; 2007: 310.

3. Brasil. Ministério da Saúde. Agência Nacional de Vigilância Sanitária (ANVISA). Resolução RDC n 259 de 20 de setembro de 2002. Aprova o Regulamento técnico sobre rotulagem de alimentos embalados. Diário Ofi cial [da] Republica Federativa do Brasil.Brasília, DF,23 de set.de 2002, Seção 1.

4. Brasil. Ministério da Marinha de Guerra, Ministério do Exército, Ministério da Aeronáutica Militar. Decreto-lei n 986 de 21 de outubro de 1969. Institui normas básicas sobre alimentos. Diário Ofi cial [da] Republica Federativa do Brasil. Brasília, DF,p. 8935 de 21 out.de1969.

5. Brasil. Ministério do Desenvolvimento, Indústria e Comercio Exterior. Instituto Nacional de Metrologia, Normalização e Qualidade Industrial (INMETRO). Portaria n 157, de 19 de agosto de 2002. Aprova o Regulamento técnico metrológico estabelecendo a forma de expressar o conteúdo líquido a ser utilizado nos produtos pré-medidos.Diário Ofi cial [da] Republica Federativa do Brasil. Brasília, DF, 20 de ago 2002, Seção 1.

6. Brasil. Congresso Nacional. Lei n° 8078 de 11 de setembro de 19990 (Código de Defesa do Consumidor). Dispõe sobre a proteção do consumidor e dá outras providencias. Diário Ofi cial [da] Republica Federativa do Brasil. Brasília, DF,12 de set de 1990, Suplemento.

7. Mosková E, Paulicková I. PCR-Based detection of cow’s milk in goat and sheep cheeses marketed in the Czech Republic. Czech Journal Food Science 2006; 24(3): 127-32.

8. Veloso ACA. Detecção de Adulteração em produtos alimentares contendo leite e/ou proteínas lácteas. Química Nova 2002; 25(4): 609-15.

9. Sgarbieri VC. Revisão: Propriedades Estruturais e Físico-Químicas das Proteínas do Leite. Brazilian Journal of Food Technology 2005; 8(1): 43-56.

10. Richter W, Krause I., Graf C, Sperrer I., Schwarzer C, Klostermeyer H. An indirect competitive ELISA for the detection of cow’s milk and caseinate in goat’s and ewes’ milk and cheese using polyclonal antibodies against bovine γ – caseins. Z Lebensm Unters Forsch A 1997; 204 : 21-6.

11. Benjamini E, Coico R, Sunshine G. Interações Antígeno-Anticorpo, Imunoensaios e Sistemas Experimentais. In: Imunologia. 4ª ed. São Paulo: Ed Guanabara Koogan S.A; 2002. p. 51-68

12. Hurley IP, Coleman RC, Ireland HE, Williams HH. Measurement of bovine igG by indirect Competitive ELISA as a means of detection milk adulteration. Journal Dairy Science 2004; 87: 543-9.

13. Borková M, Snáselová J. Possibilities of different animal milk detection in milk and dairy products – a review. Czech Journal Food Science 2005; 23(2): 41-50.

14. Asensio L., González I., García T, Martín R. Determination of food authenticity by enzyme-linked immunosorbent assay (ELISA). Food Control 2008; 19(1): 1-8.

15. Douglas FW, Greenberg R, Farrell Jr HM, Edmondson LF. Effects of ultra-high-temperature pasteurization on milk proteins. Food Agriculture Food Chemistry 1981; 29(1):11-5.

16. Pintado ME, Malcata FX. Effect of thermal treatment on the protein profi le of whey from ovine and caprine milk thoroughout lactation. International Dairy Journal 1996; 6: 497-518.

17. Haza AI, Morales P, Martín R, García T, Anguita G, Sanz B, Hernández PE. Detection and Quantifi cation of goat’s cheese in ewe’s cheese using a monoclonal antibody and two ELISA formats. Journal of the Science of food and agriculture 1999; 79: 1043-7.

18. Haasnoot W, Smits NGE, Kemmers-Voncken AEM, Bremer MGEG. Fast Biosensor immunoassays for the detection of cow’s milk in the milk of ewes and goats. Journal of Dairy Research2004; 71: 322-9.

19. Reale S, Campanella A, Mergioli A, Pilla F. A novel method for species identifi cation in milk and milk-based products. Journal of dairy Research 2008; 75:107-12.

20. Empresa Brasileira de Pesquisa Agropecuária [EMBRAPA]. Fundamentos teórico-prático e protocolos de extração e de amplificação de DNA por meio de reação em cadeia de polimerase [Recurso Eletrônico]. São Carlos: EMBRAPA Pecuária Sudeste. Disponível em: httpp://www.cppse.embrapa.br/publicacaogratuita/e-books/LVFundDNA.pdf. 2007.

21. Felligini M, Bonizzi I., Curik VC, Parma P, Greppi GF, Enne G. Detection of Adulteration in Italian Mozzarella Cheese Using Mitochondrial DNA Templates as Biomarkes. Food Techonology Biotechnology 2005; 43(1): 91-5.

22. López-Calleja I, González -Alonzo I, Fajardo V, Rodríguez, MA, Hernández PE., García T, Martín R. PCR detection of cow’s milk in water buffalo milk and Mozzarella cheese. International Dairy Journal 2005; 15: 1122-9.

23. Plath A, Krause, I., Einspanier, R. Species Identifi cation in dairy products by three different DNA-based techniques. Z Lebensm Unters Forsch A 1997; 205: 437-41.

24. Bottero, M.T., Civera, T., Nucera, D., Rosati, S., Sacchi, P., Turi, R.M. A multiplex polymerase chain reaction for the identifi cation of cow’s, gost’s and sheep’s milk in dairy products. International Dairy Journal 2003; 13: 277-82,.

25. Bania, J.; Ugorski,M.; Polanowski, A.; Adamczyk, E. Application of polymerase chain reaction for detection of goat’s milk adulteration by milk of cow. Journal of Dairy Research 2001; 68: 333-6.

26. Rea, S., Chikuni, K., Branciari, R., Sangamayya, R.S., Ranucci, D., Avellini, P. Use of duplex polymerase chain reaction (duplex – PCR) technique to identify bovine and water buffalo milk used in making mozzarella cheese. Journal of Dairy Research 2001; 68: 689-98.

27. Mafra, I.; Ferreira, I.M.P.L.V.O.; Faria, M.A.; Oliveira, B.P.P. A novel approach to the quantifi cation of bovine milk in ovine cheeses using a duplex polymerase chain reaction method. Journal of agricultural and food chemistry 2004; 52: 4943-7.

28. Feligini, M., Alim, N., Bonizzi, I., Enne, G., Aleandri, R. Detection of cow milk in water buffalo cheese by SYBR Green Real-Time PCR: Sensitivity Testo n Governing Liquid Samples. Pakistan Journal of Nutrition 2007; 6(1): 94-8.

29. Aschaffenburg, R.; Sen, A.; Thompson, P. The Casein of Buffalo Milk. Comp. Biochem. Physiol. 1968; 27: 621 – 3.

30. Otaviano, A.R.; Tonhati, H.; Sena, J.A.D.; Muñoz, M.F.C. Kappa –casein gene study with molecular markes in female buffaloes (Bubalus bubalis). Genetics and Molecular Biology 2005; 28(2): 237-41.

31. Silva, C.L.S.P. Eletroforese Bidimensional: Princípios e Aplicações. Ciências Agrárias e Saúde 2002; 2(1): 74-8.

32. Egito, A.S., Rosinha, G.M.S., Laguna, L.E., Miclo, L., Girarder, J.M., Gaillard, J.L. Método eletroforético rápido para detecção da adulteração do leite caprino com leite bovino. Arquivo Brasileiro de Medicina Veterinária e Zootecnia 2006; 58(5): 932-9.

33. Dennis, M.J. Recent developments in food authentication. Analyst 1998; 123: 151R-156R.

34. Amigo, L.; Ramos, M.; Martin-Alvarez, P.J. Effect of technology parameters on electrophoretic detection of cow’s milk in ewe’s milk cheeses. Journal Dairy Science 1991; 74: 1482-90.

35. Europeian Commission. EC 213/2001 Methods for the analysis and quality evaluation of milk and milk products. Offi cial Journal of the European Communities 2001; 44:L.37/1 –L37/99.

36. Silva, J.A.F Da. Terminologia para técnicas analíticas de eletromigração em capilares. Química Nova 2007; 30(3): 740-4.

37. Molina, E.; Frutos, M. De; Ramos, M. Capillary electrophoresis characterization of the casein fraction of cheeses made from cow’s, ewes’ and goats’ milks. Journal of Dairy Research 2000; 67: 209-16.

38. Rodriguez-Otero, J.L.; Hermida, M.; Centeno, J. Análisis of dairy produtcs by mear infraredspectroscopy: A review. Journal Agriculture Food Chemistry 1997; 45(8): 2815-9.

39. Pappas, C.S.; Tarantiles, P.A.; Moschopoulou, E.; Moatsou, G.; Kandarakis, I. Identifi cation and differenitation of goat and sheep milk based on diffuse refl ectance infrared Fourier transform spectroscopy (DRIFTS) using cluster analysis. Food Chemistry 2008; 106: 1271-7.

40. Qiaoqian, L.; Puhan, Z. Determination of protein and casein in milk by fourth derivative UV spectrosphotometry. Analytica Acta 1999; 393: 227-34.

41. Barbano, D.M.; Dellavalle, E. Rapad method for determination of milk casein content by infrared analysis. Journal of Dairy Science 1987; 70:1524-8.

42. Sato, T.; Kawano, S. Detection of Foreign fat adulteration of milk fat by near infrared spctroscopic method. Journal of Dairy Science 1990; 73: 3408-13.

43. Frankhuizen, R. Near infrared analysis of dairy products. In: Burns, D.A, Churczak, E.W. Hanbook of near infrared analysis. EUA: Ed Marcel Dekker; 1992.

44. González- Martín, I., Hernández-Hierro, J.M., Morón - Sancho, R., Salvador- Esteban, J., Vivar- Quintana, A., Revilla, I. Determination of the percentage of milk (cow’s, ewe’s and goat’s) in cheese with different ripening times using near infrared spectroscopy technology and a remote refl ectance fi bre-optic probe. Analytica Chimica Acta 2007; 604:191-6.

45. Mayer, H.K. Milk species identification in cheese varieties using electrophoretic, chomatographic and PCR techniques. International Dairy Journal 2005;15: 595-604.

46. Romero, C.; Perez-Andújar, O.; Olmedo, A.; Jiménez, S. Detection of cow’s milk in ewe’s or goat’s milk by HPLC. Chromatographia 1996; 42(3/4): 181-04.

47. Haza, A. I., Morales, P., Martin, R., Garcia, T., Anguita, G., Gonzalez, I., Sanz, B.; Hernandez, P.E. Immunoreactivity of Goat’s Milk Casein Fractionated by Ion-Exchange Chromatography. J. Agric. Food Chem. 2005; 43: 2025-9.

47. Basch, J.J., Douglas, Jr, F.W., Procino, L.G., Holsinger, V.H., Farrell, Jr, H.M. Quantitation of caseins and whey proteins of processed milks and whey protein concentrates, application of gel electrophoresis, and comparison with Harland-Ashorth Procedure. Journal Dairy Science 1985; 68(1): 23-31.

48. Anguita, G., Martín, R., García, T., Morales, P., Haza, A.I., González, I., et al. Competitive Enzyme-Linked Immunosorbent Assay for Detection of Bovine Milk in Ovine and Caprine Milk and Cheese Using a Monoclonal Antibody against Bovine -Casein. Journal of Food Protection 1997; 60(1): 64-6.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright (c) 2009 Instituto Adolfo Lutz Journal

Downloads

Download data is not yet available.