Keywords
heterotrophic bacteria
Reasoner’2 agar
Plate Count Agar
How to Cite
Abstract
To detect the potential risk which may interfere on the quality of the water used for dialysis procedure, some assessment parameters have been established led to the definition of for its evaluation, such as the counting of heterotrophic bacteria. Considering that the chosen culture media and the incubation conditions may interfere on the results from microbiological analysis on water for dialysis, the present study evaluated the efficiency of R2A culture medium for performing the heterotrophic bacteria counting. A total of 193 samples of water collected from dialysis clinics located in the city of São Paulo and in other cities of the Grande São Paulo region were analyzed, and also the incubation temperature and the efficiency comparison on bothR2A and PCA media were evaluated. No difference between bacteria counting on R2A medium after 96hincubationat 24oC and 34oC was found. The highest bacteria counting was found on R2A medium (p <0.02), which was considered to be mostly suitable for evaluating the water for dialysis. In order to minimize the risks to the patient under dialysis, the water for dialysis should be carried out by bacteria counting technique on R2A agar because of its high sensitivity, and incubating it for longer incubation times.
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