Keywords
milk
Public Health
toxin gens
How to Cite
Abstract
S. aureus isolated from diverse transmission sources during the cow milking was studied by amplifying the toxins genes. The milk samples and specimens from teat orifices were collected before milking, and samples from mouthpieces were collected before and during the milking procedure performed at varied climatic conditions. The study on the A to D enterotoxins genes and the toxin of toxic shock syndrome were carried out by amplifying the respective coding sequences. The sea gene showed to be the most prevalent among those analyzed, and the majority of isolated were detected in milk samples from mammary quarters with mastitis. The occurrence of staphylococcal toxins genes in S. aureus isolated from different sources of bovine mastitis epidemiological chain, evidences the feasibility of this microorganism transmission by contaminated milk, being a potential risk to public health.References
1. Akineden O, Annemuller, C, Hassan AA, Lammler C, Wolter W, Zschock M. Toxin genes and other characteristics of Staphylococcus aureus isolates from milk of cows with mastitis. Clin Diag Lab Immunol. 2001; 8 (5): 959-64.
2. Sabour PM, Gill JJ, Lepp D, Pacan JC, Ahmed R, Dingwell R et al. Molecular typing and distribution of Staphylococcus aureus isolates in Eastern Canadian Dairy Herds. J Clin Microbiol. 2004; 42 (8): 3449–55.
3. Zafalon LF, Langoni H, Benvenutto F, Castelani L, Broccolo CR. Aspectos epidemiológicos da mastite bovina causada por Staphylococcus aureus. Vet Zootec. 2008;15(1):56-65.
4. Harmon RJ, Eberhart RJ, Jasper DE, Langlois BE, Wilson RA. Microbiological procedures for the diagnosis of bovine udder infection. Arlington (VA): National Mastitis Council; 1990.
5. Ingawa K, Adkinson R, Gough R. Evaluation of gel teat cleaning and sanitizing compound status of the goat udder. J Dairy Res. 1992, 59: 21-8.
6. Mcdonald J, Kinsel ML, Adams DS, Darlington R.L. Studying the effect of backfl ushing milking units. Vet Med. 1993; 88: 382-6.
7. CIIAGRO. Centro Integrado de Informações Agrometeorológicas. Instituto Agronômico de Campinas, Campinas.[acesso em 2006] Disponível em: <http://ciiagro.iac.sp.gov.br/ciiagroonline/.
8. Holmberg O. Staphylococcus epidermidisisolated from bovine milk. Acta Vet Scand. 1973; 45: 1-144.
9. Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST. Gram-positive cocci. In: Bergey’s Manual Of Determinative Bacteriology. 9th ed. Baltimore: Ed. Williams e Wilkins; 1994. p. 544-51.
10. Ferreira LM, Nader Filho A, Oliveira E, Zafalon LF, Souza V. Variabilidade fenotípica e genotípica de estirpes de Staphylococcus aureus isoladas em casos de mastite subclínica bovina. Cien Rural. 2006; 36 (4): 1228-34.
11. Martineau F, Picard FJ, Roy PH, Ouellette M, Bergeron MG. Species-specific and ubiquitous-DNA-based assays for rapid identifi cation of Staphylococcus aureus. J Clin Microbiol. 1998; 36: 618-23.
12. Cunha MLRS, Calsolari RAO, Araújo Júnior JP. Detection of enterotoxin and Toxic Shock Syndrome Toxin 1 genes in Staphylococcus, with emphasis on coagulase-negative Staphylococci. Microbiol Immunol. 2007; 51 (4): 381-90.
13. Bauer AW, Kirby WMM, Truck M. Antibiotic susceptibility testing by a standardized single disc method. Am J Clin Pathol. 1966; 45: 493-6.
14. NCCLS. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing. 15th Informational Supplement. 2005, 25 (1): 177p.
15. SAMPAIO, I.B.M. Estatística aplicada à experimentação animal. Belo Horizonte: Fundação de Ensino e Pesquisa em Medicina Veterinária e Zootecnia, 1998. 221p.
16. Sá MEP, Cunha MLRS, Elias AO, Langoni H. Importância do Staphylococcus aureus nas mastites subclínicas: pesquisa de enterotoxinas e toxina do choque tóxico, e a relação com a contagem de células somáticas. Braz Journal Vet Res Anim Sci. 2004; 41 (5): 320-6.
17. Pereira ML, Pereira JL, Serrano AM, Bergdoll MS. Estafi lococos: até onde sua importância em alimentos? Hig Aliment. 2000; 14 (68-69): 32-40.
18. Gómez C, Pinal L, Franco J, Carrillo JM, Ramírez J. Identifi cation of Staphylococcus aureus strains negative for enterotoxins A, B and C isolated from bovine mastitis in México. Vet Immunol Immunopathol. 2007; 117: 249–53.
19. Silva WP, Gandra EA. Estafi lococos coagulase positiva: patógenos de importância em alimentos. Hig Aliment. 2004; 18 (122): 32-40.
20. Hata W, Katsuda K, Kobayashi H, Nishimori K, Uchida I, Higashide M et al. Bacteriological Characteristics of Staphylococcus aureus Isolates from Humans and Bulk Milk. J Dairy Sci. 2008; 91 (2): 564–9.
21. Peles F, Wagner M, Varga L, Hein I, Rieck P, Gutser K et al. Characterization of Staphylococcus aureus strains isolated from bovine milk in Hungary. Int J Food Microbiol. 2007; 118:186–93.
22. Silva ER, Carmo LS, Silva N. Detection of the enterotoxins A, B, and C genes in Staphylococcus aureus from goat and bovine mastitis in Brazilian dairy herds. Vet Microbiol. 2005; 106:103-7.
23. Silva N, Cardoso HFT, Sena MJ, Carmo LS. Produção da toxina-1 da síndrome do choque tóxico por Staphylococcus aureus isolados de leite bovino em Minas Gerais. Revista Napgama 1999; 2 (5):12-4.
24. Rall VLM, Vieira FP, Rall R, Vieitis RL, Fernandes Jr A, Candeias JMG et al. PCR detection of staphylococcal enterotoxin genes in Staphylococcus aureus strains isolated from raw and pasteurized milk. Vet Microbiol. 2008; 132: 408–13.
25. Chapaval L, Moon DH, Gomes JE, Duarte FR, Tsai SM. Use of PCR to detect classical enterotoxins genes (ent) and toxic shock syndrome toxin-1 gene (tst) in Staphylococcus aureus isolated from crude milk and determination of toxin productivities of S. aureus isolates harboring these genes. Arq Inst Biol. 2006; 73 (2): 165-9.
26. Van Leeuwen WB, Melles DC, Alaidan A, Al-Ahdal M, Boelens HAM, Snijders SV et al. Host- and tissue-specifi c pathogenic traits of Staphylococcus aureus. J Bacteriol. 2005; 187 (13): 4584–91.
27. Brabes KCS, Carvalho EP, Dionísio FL, Pereira ML, Garino Júnior F, Costa EO. Participação de espécies coagulase positivas e negativas produtoras de enterotoxinas do gênero Staphylococcus na etiologia de casos de mastite bovina em propriedades de produção leiteira dos Estados de São Paulo e Minas Gerais. Revista Napgama 1999; 2 (3): 4-11.
28. Guedes Neto LG, Veras JF, Sena MJ, Cerqueira MMOP, Santos WLM,Souza MR et al. Isolamento e identificação de Staphylococcusspp. e suas enterotoxinas de amostras de queijo de coalho produzidos em Pernambuco, Brasil. Hig Aliment. 2005; 19 (130) (encarte eletrônico).
29. Vieira-da-Motta O, Folly MM, Sakyiama CCH. Detection of different Staphylococcus aureus strains in bovine milk from subclinical mastitis using PCR and routine techniques. Braz J Microbiol. 2001; 32:27-31.
30. Morandi S, Brasca M, Lodi R, Cremonesi P, Castiglioni B. Detection of classical enterotoxins and identification of enterotoxin genes in Staphylococcus aureus from milk and dairy products. Vet Microbiol. 2007; 124:66-72.
31. Zecconi A, Cesaris L, Liandris E, Dapra V, Piccinini R. Role of several Staphylococcus aureus virulence factors on the inflammatory response in bovine mammary gland. Microb Pathogen. 2006; 40: 177-83.
32. Kenny K, Reiser RF, Bastida-Corcuera FD, Norcross NL. Production of enterotoxins and toxic shock syndrome toxin by bovine mammary isolates of Staphylococcus aureus. J Clin Microbiol. 1993; 31 (3): 706-7.
33. Fagundes, H, Oliveira, CAF. Infecções intramamárias causadas por Staphylococcus aureus e suas implicações em saúde pública. Cien Rural. 2004; 34 (4): 1315-20.
34. Araújo WP. Fagotipagem de cepas de Staphylococcus aureusresistentes a antibióticos, isoladas de leite. Braz J Vet Res Anim Sci. 1998; 35 (4): 161-5.
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