Keywords
OTA
raisins
HPLC
immunoaffinity column
How to Cite
Abstract
The present study aimed to evaluate a methodology for analyzing ochratoxin A (OTA) in raisins samples marketed in São Paulo, Brazil. OTA was extracted with methanol:water (80:20, v/v) and diluted with phosphate buffer solution, and purified through an immunoaffinity column. OTA was separated and quantified using HPLC with fluorescence detection. The established detection and quantification limits were 0.24 and 0.80 ng/g, respectively. The recoveries values were 81.6, 80.4 and 81.9%, and the relative standard deviations (RSD) were 6.0, 4.3, and 6.1 % at 2.0, 5.0 and 10.0 ng/g levels, respectively. Of twenty black raisin samples analyzed 10 (50 %) contained OTA at levels ranging from 1.3 to 39.1 ng/g. All of twenty-two white raisin samples showed no contamination with OTA.References
1. WHO. World Health Organization, Ochratoxin A –Toxicological Evaluation of Certain Food Additives and Contaminants, WHO Food Additives Series 35 1996, Geneva, Switzerland, 363-376.
2. Schlatter CH, Studer-Rohr J, Rasonyi TH. Carcinogenicity and kinetid aspects of ochratoxin A. Food Addit Contam 1996; 13: 43-4.
3. Pittet A. Natural occurrence of mycotoxins in foods and feeds: an updated review. Rev Med Veter 1998; 149: 479-92.
4. Petkova-Bocharova T, Castegnaro M In: Mycotoxins, Endemic Nephropathy and Urinary Tract Tumours, Castegnaro M, Plestina R, Dirheimer G, Chernozemsky IN, Bartsch H editors. IARC Press: Lyon, France, 1991;p.159-64.
5. International Agency for Research on Cancer [IARC]. Ochratoxin A. In: IARC Monographs on the Evaluation ofCarcinogenic Risks to Human; Some Naturally Occurring Substances; Food Items and Constituents, Heterocyclic Aromatic Amines and Mycotoxins. IARC: Lyon, 1993,p.489-521.
6. Sage L, Krivobok S, Delbos E, Seigle-Murandi F, Creppy EE. Fungal flora and ochratoxin A production in grapesand Musts from France. J Agric Food Chem 2002; 50:1306-11.
7. Serra R, Abrunhosa L, Kozakiewicz Z, Venâncio A. Black Aspergillus species as ochratoxin A producers in Portuguese wine grapes. Int J Food Microbiol 2003; 88: 63-8.
8. FAO/WHO, Safety evaluation of certain mycotoxins infood. WHO Food Additives Series, No. 47; FAO Food and Nutrition Paper 74, 2001.
9. Trucksses MW, Maragos CM. Joint Mycotoxin Committee. Technical committee reports. JAOAC Int 2001; 84:303-8.
10. Stefanaki I, Foufa E, Tsatsou-Dritsa A, Dais P. Ochratoxin A concentration in Greek domestic wines and dried vinefruits. Food Addit Contam 2003; 20: 74-83.
11. MAFF (Ministry of Agriculture, Fisheries and Food). Survey of retail products for ochratoxin A. Food Safety Information Bulletin 1998, n.185.
12. Ianamata BT. Fungos toxigênicos e micotoxinas em frutas secas e produção de ocratoxina A em uvas passas em condições de abuso [Dissertação de Mestrado]. Campinas, São Paulo: Universidade Estadual de Campinas, 2004. 64pp.
13. Ianamata BT, Taniwaki MH, Menezes HC, Vicente E, Fungaro MH. Incidence of toxigenic fungi and ochratoxin A in dried fruits sold in Brasil. Food Addit Contam 2005;22: 1258-63.
14. AOAC International. Official Methods of Analysis, 16 thed., Washington, DC: Association of Official Analytical Chemists, 1997, ch. 49.
15. Commission of the European Communities. Commission Directive (EC No 26/2002 of 13 March 2002). Off J European Communities 2002, 75: 38.
16. Monaci L, Palmisano F. Determination of ochratoxin A infoods: state-of-the-art and analytical challenges. Anal Bioanal Chem 2004; 378: 96-103.
17. Magnoli C, Astoreca A, Ponsone L, Combina M, Palácio G, Rosa CAR, Dalcero AM. Survey of mycoflora and ochratoxin A in dried vine fruits from Argentina markets .Lett Applied Microbiol 2004; 39: 326-31.
18. MacDonald S, Wilson P, Barnes K, Damant A, Massey R, Mortby E, Shepherd MJ. Ochratoxin A in dried vine fruit: method development and survey. Food Addit Contam 1999;16: 253-60.
19. Commission of the European Communities. Commission regulation (EC 9 n0 472/2002 of 12 March 2002 amending regulation (EC) n0 466/2002). Off J European Communities 2002, 75: 18-20.
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