Biological safety cabinet: ultraviolet radiation effect on mycobacteria
PDF (Português (Brasil))

Keywords

biological safety cabinet
UV radiation
mycobacteria

How to Cite

1.
Ueki SYM, Geremias AL, Moniz LL, Latrilha FO, Brito AC de, Giampaglia CMS, Simeão FC dos S, Telles MAS. Biological safety cabinet: ultraviolet radiation effect on mycobacteria. Rev Inst Adolfo Lutz [Internet]. 2006 Aug. 1 [cited 2024 Nov. 23];65(3):222-4. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/32870

Abstract

Biological safety cabinet (BSC) is the major equipment which is the primary means of containment of infectious microorganisms aerosols generated from laboratory procedures, and the decontamination using ultraviolet radiation , constitute a stage of Good Laboratory Practices. The objective of this study was to evaluate the effect of UV lamp of class II B2 cabinet on varied mycobacteria species and to correlate them with UV exposition time. Reference strains were subcultivated and incubated at 37º Cuntil getting a turbidity equivalent to that of MacFarland 1 standard. From this bacterial suspension, 100μL were plated into 7H11 medium; the plates were partially covered with aluminium paper and exposed to UV radiation for 5 and 10 minutes. After UV exposition, the paper was removed and the plates were incubated at 37ºC for 30 days. All plates showed no bacterial growth at the side exposed to the UV radiation, while a regular and abundant bacteria growth was observed at the other half part that was covered with paper. Switching on the UV lamp for 15 minutes after using the cabinet and for 15 minutes before starting any activity inside the cabinet guarantees proper decontamination. This biosafety practice is recommended to decontaminate the BSC and also materials that need to be withdrawn from the cabinet.
https://doi.org/10.53393/rial.2006.65.32870
PDF (Português (Brasil))

References

1. Brasil. Ministério da Saúde. Fundação Nacional de Saúde. Secretaria de Vigilância em Saúde. Departamento de Vigilância Epidemiológica. Biossegurança em laboratórios biomédicos e de microbiologia. Brasília, 2005.

2. Brasil. Ministério da Saúde. Secretaria de Ciência, Tecnologia e Insumos Estratégicos. Diretrizes gerais para o trabalho em contenção com material biológico. Brasília, 2004.

3. Organización Panamericano de la Salud. Oficina Sanitária Panamericana, Oficina Regional de la Organización Mundial de la Salud. Cabinas de seguridad biológica: uso,desinfección y mantenimiento. 2002.

4. Ferreiros M. Cabines de segurança biológica.(www.sbcc.com.br). Rev Elet SBCC 2001; 14: 16-225.

5. Mastroeni M.F. Biossegurança aplicada a laboratórios e serviços de saúde. São Paulo, Editora Atheneu, 2004.

6. Brasil. Leis, etc. Portaria no 930, de 27 de agosto de 1992. Dispõe sobre normas para controle das infecções hospitalares. Diário Oficial [da] União, Brasília, Seção 1,pp. 12279-81, 04 de setembro de 1992.

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Copyright (c) 2006 Instituto Adolfo Lutz Journal

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