Listeria monocylogenes em hortaliças: isolamento e sorotipagem

Marcelo Luiz Lima Brandão; Fernanda Corredeira da Paixão Bispo; Davi de Oliveira Almeida; Carla de Oliveira Rosas; Silvia Maria Lopes Bricio; Victor Augustus Marin

doi:10.18241/0073-98552013721549

Vol. 72 No. 1 (2013), BRIEF COMMUNICATION

Vol. 72 No. 1 (2013)

Listeria monocytogenes in green vegetables: isolation and serotyping

BRIEF COMMUNICATION

https://doi.org/10.18241/0073-98552013721549

Published 2013-01-22

Marcelo Luiz Lima Brandão⁺⁻
Fernanda Corredeira da Paixão Bispo⁺⁻
Davi de Oliveira Almeida⁺⁻
Carla de Oliveira Rosas⁺⁻
Silvia Maria Lopes Bricio⁺⁻
Victor Augustus Marin⁺⁻

Marcelo Luiz Lima Brandão

Fundação Oswaldo Cruz, Instituto Nacional de Controle de Qualidade em Saúde, Departamento de Microbiologia, Laboratório de Microbiologia de Produtos, Setor de Alimentos, Rio de Janeiro, RJ

Fernanda Corredeira da Paixão Bispo

Fundação Oswaldo Cruz, Instituto de Controle de Qualidade em Saúde, Rio de Janeiro, RJ

Davi de Oliveira Almeida

Fundação Oswaldo Cruz, Instituto de Controle de Qualidade em Saúde, Rio de Janeiro, RJ

Carla de Oliveira Rosas

Fundação Oswaldo Cruz, Instituto de Controle de Qualidade em Saúde, Rio de Janeiro, RJ

Silvia Maria Lopes Bricio

Fundação Oswaldo Cruz, Instituto Nacional de Controle de Qualidade em Saúde, Departamento de Microbiologia, Laboratório de Microbiologia de Produtos, Setor de Alimentos, Rio de Janeiro, RJ

Victor Augustus Marin

Fundação Oswaldo Cruz, Instituto de Controle de Qualidade em Saúde, Rio de Janeiro, RJ

PDF (Português (Brasil))

Keywords

Listeria monocytogenes
leafy green vegetables
serotyping

How to Cite

1.

Brandão MLL, Bispo FC da P, Almeida D de O, Rosas C de O, Bricio SML, Marin VA. Listeria monocytogenes in green vegetables: isolation and serotyping. Rev Inst Adolfo Lutz [Internet]. 2013 Jan. 22 [cited 2024 Jul. 22];72(1):99-103. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/32902

Abstract

Consumption of food contaminated with Listeria monocytogenes poses as a risk to human health. Ninety-seven samples of green leafy vegetables marketed in the state of Rio de Janeiro were analyzed for detecting L. monocytogenes by selective enrichment. Thirty-five in natura green vegetables, 32 minimally processed samples and 30 ready-to-eat raw salads samples were assessed. The isolates were serotyped by means of classical seroagglutination technique using somatic and flagellar polyclonal antisera. Seven samples (7.2 %) showed to be contaminated with L. monocytogenes, being two in natura samples and five ready-to-eat salads. All isolates were identified as serovar 1/2a. Considering these findings on the contamination of in natura green vegetables with L. monocytogenes, the consumers should be advised regarding to the possibility of this kind of product to be contaminated with this pathogen, and also to imply vigilance in observing the proper sanitization procedures to avoid the cross-contamination with other foods. Occurrence of L. monocytogenes in ready-to-eat salad samples denotes a health hazard to consumers, especially to those who belong to risk groups.

https://doi.org/10.18241/0073-98552013721549

PDF (Português (Brasil))

References

1. Mecanoglu TB, Halkman AK. Do leafy green vegetables and their ready-to-eat [RTE] salads carry a risk of foodborne pathogens? Anaerobe.2011;17(6):286-7.

2. Food and Agricultural Organization/World Health Organization – FAO/WHO. Microbiological hazards in fresh leafy vegetables and herbs. Meeting Report. Rome: WHO, 2008. 151p. Microbiological Risk Assessment Series Nº 14. [acesso 2012 Abr 26]. Disponível em: [http://ftp.fao.org/docrep/fao/011/i0452e/i0452e00.pdf ].

3. Newell DG, Koopmans M, Verhoef L, Duizer E, Aidara-Kane A, Sprong H et al. Food-borne diseases – The challenges of 20 years ago still persist while new ones continue to emerge. Int J Food Microbiol.2010;139(Supl. 1):S13-5.

4. Hitchins AD, Jinneman K. Detection and Enumeration of Listeria monocytogenes in Foods. In: Bacteriological analytical manual Online, Charper 10. [S.l]; FDA. 2011. [acesso 2012 Abr 26]. Disponível em: [http://www.fda.gov/Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/ucm071400.htm].

5. Swaminaphan B, Cabanes D, Zhang W, Cossart P. Listeria monocytogenes. In: Doyle MP, Beuchat LR. Food Microbiology Fundamentals and Frontiers. 3rd Ed. Washington DC: ASM Press, 2007. p. 457-91.

6. Brasil. Ministério da Saúde. Resolução RDC n.º12, de 2 de janeiro de 2001. Aprova regulamento Técnico sobre Padrões Microbiológicos para alimentos e seus anexos I e II. Diário Oficial [da] República Federativa do Brasil. Brasília, DF, n.7-E, 10 jan. 2001. Seção1, p. 45.

7. Seeliger HPR, Höhne K. Serotyping of Listeria monocytogenesand related species. Meth Microbiol.1979;13:31-49.

8. De Giusti M, Aurigemma C, Marinelli L, Tufi D, Medici D, Pasquale S, et al. The evaluation of the microbial safety of fresh ready-to-eat vegetables produced by different technologies in Italy. J Appl Microbiol.2010;109:996-1006.

9. Abadias M, Usall J, Anguera M, Solsona C, Viñas I. Microbiological quality of fresh, minimally-processed fruit and vegetables, and sprouts from retail establishments. Int J Food Microbiol.2008;123:121-9.

10. Tresseler JFM, Figueiredo EAT, Figuereido RW, Machado TF, Delfino CM, Sousa PHM. Avaliação da qualidade microbiológica de hortaliças minimamente processadas. Ciênc Agrotecnol.2009;33(Edição Especial):1722-7.

11. Oliveira M, Usall J, Viñas I, Anguera M, Gatius F, Abadias M. Microbiological quality of fresh lettuce from lettuce from organic and conventional production. Food Microbiol.2010;27:679-84.

12. Fröder H, Martins CG, Souza KLO, Landgraf M, Franco BDGM, Destro MT. Minimally Processed Vegetable Salads: Microbial Quality Evaluation. J Food Protect.2007;70(5):1277-80.

13. Cordano AM, Jacquet C. Listeria monocytogenes isolated from vegetables salads sold at supermarket in Santiago, Chile: Prevalence and strain characterization. Int J Food Microbiol.2009;132:176-9.

14. Little CL, Taylor FC, Sagoo SK, Gillespie IA, Grant K, McLauchlin J. Prevalence and level of Listeria monocytogenes and other Listeria species in retail pre-packaged mixed vegetable salads in the UK. Food Microbiol.2007;24:711-7.

15. Oliveira ABA, Ritter AC, Tondo EC, Cardoso MI. Comparison of Different Washing and Disinfection Protocols Used by Food Services in Southern Brazil for Lettuce (Lactuca sativa). Food Nutr Sci.2011;3:28-33.