Analysis of in vitro antimicrobial activity of antiseptics products by means of time kill assay
Vol. 72 No. 3 (2013), ORIGINAL ARTICLE
Vol. 72 No. 3 (2013)

Analysis of in vitro antimicrobial activity of antiseptics products by means of time kill assay

ORIGINAL ARTICLE
https://doi.org/10.18241/0073-98552013721568
Published 2013-03-22
Hilda do Nascimento Nobrega
Fundação Oswaldo Cruz, Instituto Nacional de Controle de Qualidade em Saúde, Rio de Janeiro, RJ
Joana Angélica Barbosa Ferreira
Fundação Oswaldo Cruz, Instituto Nacional de Controle de Qualidade em Saúde, Rio de Janeiro, RJ
Célia Maria Maria Carvalho Pereira Araújo Romão
Fundação Oswaldo Cruz, Instituto Nacional de Controle de Qualidade em Saúde, Rio de Janeiro, RJ
Ivano Raffaele Victorio de Filippis Capasso
Fundação Oswaldo Cruz, Instituto Nacional de Controle de Qualidade em Saúde, Rio de Janeiro, RJ
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Keywords

antimicrobial activities
antiseptic
time kill
neutralization

How to Cite

1.
Nobrega H do N, Ferreira JAB, Romão CMMCPA, Capasso IRV de F. Analysis of in vitro antimicrobial activity of antiseptics products by means of time kill assay. Rev Inst Adolfo Lutz [Internet]. 2013 Mar. 22 [cited 2024 Dec. 4];72(3):226-33. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/32922
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Abstract

Many chemicals (alcohol, iodophor, chlorhexidine, etc) are used in laboratories and industries. Unlike disinfectants, no specific norms and criteria have been standardized for evaluating the antiseptics activity. This study analyzed the antimicrobial activity of antiseptics using Time Kill Test (Hobson & Bolsen). This assay assessed the evolution of a population of aerobic microorganisms in a specific period of time when tested against antimicrobial agents. Two different recovery methodologies were evaluated: membrane filtration and pour plate technique The membrane filtration assay was less sensitive. Pour plate technique showed high sensitivity with high colonies counts. Of 25 samples of products analyzed, only the chlorhexidine digluconate-based antiseptics were unsatisfactory, showing no efficacy on all reference micro-organisms strains, and corresponded to 20 % of analyzed samples. However, they were efficacious against clinical strains. Therefore, these products should be used with caution and further studies are needed, as data on its efficacy have still been scarce. These findings might give support to the health surveillance and public health in establishing the future legislation, as these products have been available on the market, but without following any specific legislation.
https://doi.org/10.18241/0073-98552013721568
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