Keywords
fumonisins
corn
nutrients
mycoflora
How to Cite
Abstract
The present study aimed to correlate the mycoflora and mycotoxins production with nitrogen, zinc, and boro levels used during the corn grain crop. For the experiment, following treatments were done: use of four doses of nitrogen (0, 50, 100, and 150 kg/ha), two levels of zinc (0.5 and 1.0 kg/ha), two of boro (0.25 and 0.5 kg/ha), and two l evels of zinc and boro (0.5 and 1.0 , 0.25 and 0.5 kg/ha, respectively). Twenty-five treatments were performed. The corn mycoflora profiles showed that Aspergillus , Penicillium and Fusarium contaminations prevailed in 42.7, 38.9, and 41.5% of samples, respectively. The highest frequencies of isolated fungal were detected mainly at a w values of 0.53-0.63. Fumonisins analysis indicated a contamination in 100% of corn samples, at concentrations ranging from 1.7 to 27.9 mg/kg for FB 1 , and from 0.3 to 11.2 mg/kg for FB 2 . Seven corn grain samples were contaminated with aflatoxin B 1 at concentrations ranging from 16.0 to 1,858.3 mg/kg, and three samples were contaminated with aflatoxin B 2 at concentrations that ranged from 14.6 to 110.3 mg/kg. Statistical analysis indicated that the nitrogen did influence on the prevalence of Fusarium spp. in corn grains (p<0.05). A significant negative correlation was observed between nitrogen and the genus Aspergillus prevalence in corn grains (p<0.10).
References
1. Pittet A. Natural occurrence of mycotoxins in foods and feeds – an updatedreview. Revue Méd Vét 1998; 149:479-92.
2. Gelderblom WCA et al. Fumonisins: novel mycotoxin with câncer-promoting activity produced by Fusarium moniliforme. Appl EnvironMicrobiol 1988; 54: 1806-11.
3. International Agency on Research on Cancer [IARC]. Some naturallyoccurring substances: food items and constituents, heterocyclic aromaticamines and mycotoxins. IARC. Monographs on the evaluation ofcarcinogenic risks to humans. Lyon: IARC, 1993. v.56.
4. Diener UL, Cole RJ, Sanders TH, Payne GA, Lee LS, Klich MA.Epidemiology of aflatoxin formation by Aspergillus flavus. Ann Rev Phytopathol 1987; 25: 249-70.
5. Stack RW, Horst RK, Langhans RW. Effects of nitrogen and potassiumfertilization on infection of florists carnation by Giberella zeae. PlantDisease 1986; 70:29-31.
6. Amézquita MO, Barrera AC, Arbeláez G, Granada EG, Ospira J. Evaluciondos sistemas de desinfeccion del suelo y su interaccion com algunasformulaciones de microelementos sobre la incidencia de Fusariumoxysporium en dos variedades de clavel. Agronomia Colombiana, 1993;10:122-7.
7. Cuero RG, Osuji G, Washington A. N-carboxymethylchitosan inhibitionof aflatoxin production: role of zinc. Biotechnol Letters 1991; 13: 441-4.
8. Büll LT. Nutrição Mineral do Milho. In: Cultura do Milho. Simpósio sobrefatores que afetam a produtividade do milho e sorgo. Piracicaba: Associação Brasileira para Pesquisa da Potassa e do Fosfato, 1990: 63-145.
9. Miller R, Donahue R. Potassium, sulfur, and micronutrients. In Soils: Anintroduction to soils and plant growth 6th ed. New Jersey: Prentice-HallInternational Inc.; 1990, p.281-307.
10. Reid LM, Zhu X, Ma BL. Crop rotation and nitrogen effects on maizesusceptibility to Gibberella (Fusarium graminearum) ear rot. Plant andsoil 2001; 237: 1-14.
11. Borkert CM. Micronutrientes na planta. In: Bull LT, Rosolem CA editores. Interpretação de análise química do solo e planta para fins de adubação. Botucatu: Fundação de Estudos e Pesquisas Agrícolas Florestais; 1989:p.309-29.
12. Graham RD. Effects of nutrient stress on susceptibility of plants to diseasewith particular reference to the trace elements. Adv Bot Research 1983;10:221-76.
13. Failla LJ, Lynn D, Niehaus GJ. Correlation of Zn2+ content with aflatoxincontent of corn. Appl Environ Microbiol 1989; 52:73-4.
14. Jackson MA, Slininger PJ, Bothast RJ. Effects of zinc, iron, cobalt and manganeseon Fusarium moniliforme NRRL 13616 growth and fusarin C biosynthesis insubmerged cultures. Appl Environ Microbiol 1989; 55: 649-55.
15. Griffin DH. Fungal Physiology. New York: Wiley Interscience Publication;1993.
16. Cuero RG. Regulation of mycotoxins formation and fungal growth by metalions and fertilizer: Effect on fungal gene expression. In: Koe WJ. editor.Mycotoxins and Phycotoxins in perspective at the turn of the millennium. Guarujá, 2000. p. 355-361.
17. Yamada T. Boro: será que estamos aplicando a dose suficiente para oadequado desenvolvimento das plantas? Informações Agronômicas -POTAFOS 2000; 90:1-5.
18. Berjak P. Report of seed committee working group on the effects of storagefungi on seed viability. Seed Sci Technol 1984; 12: 233-53.
19. Raper KB, Fennell DI. The genus Aspergillus. Baltimore: Willians & Wilkins; 1965.
20. ARX JA von. The genera of fungi sporulating in pure culture. Vaduz: J.Cramer; 1974.
21. Nelson PE, Touson TA, Marasas WFO. Fusarium species. An ilustradedmanual for identification. Pennsylvania: University Press; 1983.
22. Nelson PE. Taxonomy and biology of Fusarium moniliforme.Mycopathologia 1992; 117:29-36.
23. Sydenham EW, Shephard GS, Thiel PG, Snijman PW, Stockenstrom S. Liquid chromatographic determination of fumonisin B1, B2 and B3 incorn: AOAC-IUPAC collaborativy study. J Assoc Off Anal Chem Int 1996;79: 688-96.
24. Soares LMV, Rodriguez-Amaya DB. Survey of aflatoxins, ochratoxin A, zearalenone and sterigmatocystin in some Brazilian foods by using multi-toxin thin layer chromatographic method. J Assoc Off Anal Chem 1989;72: 22-6.
25. Draper NR, Smith H. Applied regression analysis. New York: John Wiley;1981.
26. Lacey J. Water availability and the ocorrence of toxigenic fungi andmycotoxins in stored products. In: International IUPAC Symposium onMycotoxins and Phycotoxins, 6. Anais Tokyo, 1988:186-89.
27. Cahagnier B, Melcion D, Richard-Molard D. Growth of Fusariummoniliforme and its biosynthesis of fumonisin B1 on maize grain as afunction of different water activities. Lett Appl Microbiol 1995; 20: 247-51.
28. Marin S, Sanchis V, Arnau F, Ramos AJ, Magan N. Colonisation andcompetitiveness of Aspergillus and Penicillium species on maize in thepresence of Fusarium moniliforme and Fusarium proliferatum. Int J FoodMicrobiol 1998; 45: 107-17.
29. Almeida AP, Corrêa B, Direito GM, Fonseca H, Fancelli AL, Ortega E.Mycoflora and fumonisin contamination in Brazilian corn from sowing toharvest. J Agric Food Chem 2002; 50:3877-82.
30. Miller JD. Epidemiology of Fusarium ear diseases of cereals. In: Miler JD, Trenholm HL editores. Mycotoxins in grain. St Paul: Eagan Press;1994. p.19-36.
31. Duffy BK, Defago G. Trace mineral amendements in agriculture foroptimizing the biocontrol activity of plant associated bacteria. In: Berthelin,Huang PM, Bollag JB, Andreaux F editores. Effect of mineral-organic microorganism interaction on soil and freshwater environments. New York:Kluwer Academic/Plenum Publishers; 1999. p.295-304.
32. Celar, F. Competition for ammonium and nitrate forms of nitrogen betweensome phytopathogenic and antagonistic soil fungi. Biological Control 2003,1-6
33. Sarathachandra U, Ghani A, Waller, J Burch, G Sayer S, Waipara N, DexterM. Impact of carbon-rich dairy factory effluent on growth of perennialryegrass (Lolium perenne) and soil microorganisms. European Journal ofSoil Biology 2006, 42:13-22.
34. Thompson JE, Legge R, Baber RL. The role of free radicals in senescenceand wounding. New Phytol 1987; 105:317-44.
35. Zubaidi A, Mcdonald GK, Hollamby GJ. Nutrient uptake and distributionby bread and durum wheat under drought conditions in South Australia.Australian J Experim Agriculture 1999; 39:721-32.
36. Guerra D, Anderson AJ. The effect of iron and boron amendments oninfection of bean by Fusarium solani. Physiol Biochem 1985; 75:989-91.
37. Anderson HW, Nehring EW, Wichser WR. Aflatoxin contamination of cornin the field. J Agric Food Chem 1975; 23:775-82.
38. Jones RK, Ducan HE. Effect of nitrogen fertilizer, planting date, and harvestdate on aflatoxin production in corn inoculated with Aspergillus flavus.Plant Disease 1981; 65: 741-4.
39. Bassir O, Adekunle AA. Production of aflatoxin B1 from defined naturalcultures of Aspergillus flavus (Link). Mycopathol Mycol Appl 1972;46:421-6.
40. Obidoa O, Ndubuisi IE. The role of zinc in the aflatoxigenic potential of Aspergillus flavus NRRC 3251 on foods tuffs. Mycopathol 1981; 74:3-6.
41. Cuero R, Ouellet T. Metal ions modulate gene expression and accumulation of the mycotoxins aflatoxin and zearalenone. J Appl Microbiol 2005;98:598-605.
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