Keywords
DON
wheat
mycotoxins
How to Cite
Abstract
Twenty-eight samples of wheat flour and 14 samples of wheat were purchased in the city of São Paulo and these products were analyzed for deoxynivalenol (DON). Samples were extracted with acetonitrile-water (84+16) followed by a cleanup with MycoSep column. The extract purification was carried out on TLC and the toxin was determined with AlCl 3 solution. The efficiency of MycoSep columns 225 and 227 was evaluated with samples of wheat and wheat flour spiked with DON at two levels, 80.0 and 100.0 ¼g/kg. The recovery averages on columns 225 and 227 were, respectively, 72% and 107% for wheat flour and 90% and 125% for wheat grain, respectively. The relative standard deviations were 11% and 18% for wheat flour and 8% and 20% for wheat grain, respectively. DON was detected in 19 (45%) of 42 analysed samples at levels ranged from 82 to 1500 ¼g/kg. Eight samples were confirmed by means of HPLC-UV.
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