Keywords
glucose concentration
inoculum
culture medium
vaccine
polysaccharide
How to Cite
Abstract
Polysaccharide for producing a specific vaccine against N. meningitidis (serogroup C) is the principal product obtained from this microorganism cultures. In order to assess the polysaccharide production varying the bacteria inoculum bulk ,and the initial glucose concentrations, four assays were carried out in duplicate based on factorial 22 planning design. Samples were collected from the cultures every two hours for determining cell concentration (expressed as dry biomass), pH, glucose and polysaccharide concentration. The comparative criteria were based on the final polysaccharide concentrations, and the cell/polysaccharide (YP/X) yield coefficient. The bacteria culture employing the highest inoculum bulk (optical density ≅ 0.1 at 540nm) presented the best result of final polysaccharide concentration (0.105 g/ L), and YP/X (0.078). The polysaccharide further purified on gel chromatography column following the molecular mass criterion, was the suitable antigen for vaccine production.References
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