Keywords
potassium dichromate
ecotoxicology
bioassay/methods
How to Cite
Abstract
The ecotoxicology analyses determine the deleterious effect caused by a toxic agent on an organism .Microcrustacean Daphnia magna is one of the most used organisms for performing toxicity bioassay. To guarantee the reliability of results, evaluation of the dafnias sensitivity is required as they can be influenced by the culture conditions.The present work evaluated the sensitivity testing performed from March 2004 to March 2005 in the Laboratory of Ecotoxicology of Instituto de Pesquisas Ambientais e Tecnológicas(IPAT)-University do Extremo Sul Catarinense (UNESC), following the criteria from ABNT NBR 12713 norm. The potassium dichromate in a concentration of 10 mg/L was used as reference substance to get the sensitivity band. The organisms aged from 2 to 24 hours were tested in solutions containing different concentrations of potassium dichromate. The CE(I)50 mean value of 1.28 mg/L indicates that it is within the potassium dichromate band limit, in accordance to ISO 6341 norm criteria. Comparing this finding with the value established by ISO and with the data reported by other authors, it can be inferred that the dafnias cultivated in the Laboratory of Ecotoxicology at IPAT have been suitable for being employed on toxicity assayss
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