Keywords
molecular methods
bacterial genotyping
PFGE
How to Cite
Abstract
PFGE ("pulsed field gel electrophoresis") is an electrophoresis technique suitable for separating large fragments of DNA by means of DNA reorientation in agarose gel from the effects of alternated electric fields. This technique is considered as gold standard for performing bacteria, yeast and protozoa strains identification. The main objective of the present review is to update the PFGE technical basis for being suitable for bacteria studies. Its resolution depends on a series of factors such as agarose concentration, temperature, buffer solution, pulse time, and running time. Understanding the involved molecular mechanisms in this type of electrophoresis technique is fundamental for maximizing and attaining accurate analysis results.References
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