Keywords
goat milk
TLC technique
occurrence
How to Cite
Abstract
Aflatoxin M1 (AFM1) is a biotransformation product from aflatoxin B1, which is excreted in milk from animals that ingest contaminated foodstuff. The objectives of this study were to improve the thin layer chromatography (TLC) technique for analyzing AFM1 in goat milk, and to evaluate its occurrence in samples from State of Bahia. One hundred raw milk samples were colleted from five farms, located at the Recôncavo Baiano, during the period from November 2000 to August 2002. Milk samples were analyzed for determining aflatoxin M1 by means of TLC technique (Sabino et al. 1989) with some modifications. For that, lead acetate was employed during the purification process in order to precipitate proteins found in substrate, and allowed the visualization of AFM1 on chromatography plate. By means of modified TLC technique the detection and quantification limits of aflatoxin M1 were of 0.2 and 0.5 µg/L, respectively. The percentage of recoveries average and coefficient of variation for AFM1 detection were 89.6 e 0%, respectively. These results revealed that this technique was efficient for determining AFM1 in goat milk. AFM1 was not detected in any of the analyzed samples, demonstrating the good quality of these products regarding to contamination by this toxin.References
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