Importância do diagnóstico laboratorial na busca de casos de tuberculose em um hospital psiquiátrico

Hindenburg C. G. da Costa; Ana Carolina Malaspina; Fernando F. de Mello; Clarice Q. F. Leite

doi:10.53393/rial.2005.64.32993

Vol. 64 No. 2 (2005), ORIGINAL ARTICLE

Vol. 64 No. 2 (2005)

Searching tuberculosis cases in a psychiatric hospital: importance of laboratory diagnosis

ORIGINAL ARTICLE

https://doi.org/10.53393/rial.2005.64.32993

Published 2005-02-10

Hindenburg C. G. da Costa⁺⁻
Ana Carolina Malaspina⁺⁻
Fernando F. de Mello
Clarice Q. F. Leite⁺⁻

Hindenburg C. G. da Costa

Laboratório Hormonal, Rio Verde, GO

Ana Carolina Malaspina

Universidade Estadual Paulista, Faculdade de Ciências Farmacêuticas, Departamento de Ciências Biológicas, Araraquara, SP

Fernando F. de Mello

Clarice Q. F. Leite

Universidade Estadual Paulista, Faculdade de Ciências Farmacêuticas, Departamento de Ciências Biológicas, Araraquara, SP

PDF (Português (Brasil))

Keywords

psychiatric hospital
polymerase chain reaction technique
Mycobacterium tuberculosis
Mycobacterium chelonae
mycolic acids
drug sensibility

How to Cite

1.

da Costa HCG, Malaspina AC, de Mello FF, Leite CQF. Searching tuberculosis cases in a psychiatric hospital: importance of laboratory diagnosis. Rev Inst Adolfo Lutz [Internet]. 2005 Feb. 10 [cited 2024 May 15];64(2):258-62. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/32993

Abstract

From February to August of 2002, the search for M. tuberculosis was performed among 74 inpatients from the Psychiatric Hospital of Goiás - Brazil. Mycobacteria were isolated from five (6.8%) patients, being four M. tuberculosis isolates and one M. chelonae. M. chelonae positive result on baciloscopy was observed in a sputum sample only. M. tuberculosis was isolated culture of clinical specimens from four smearnegative patients. Mycobacteria species were identified by means of conventional methods, mycolic acids analysis, and PCR technique. The most specific and sensitive method - PCR detected M. tuberculosis on a culture and M. chelonae was only identified by means of conventional methods. According to drug susceptibility profiles, all of four M. tuberculosis isolates were susceptible to isoniazid and riphampicin.

https://doi.org/10.53393/rial.2005.64.32993

PDF (Português (Brasil))

References

1. Raviglione MC, Snider DE, Kochi A. Global Epidemiology of Tuberculosis: Morbidity and Mortality of a Worldwide Epidemic. JAMA1995; 273: 220-6.

2. CNPq. Cientistas declaram guerra à tuberculose. CNPq notícias [citadoem 2 out 2002]. Disponível em: URL: http//www.cnpq.br/notícias/130902.htm

3. Rasolofo-Razanamparany V, Menard D, Ratsitorahina M, Auregan G,Gicquel B, Chanteau S. Transmission of tuberculosis in the prison of Antananarivo (Madagascar), Res Microbiol 2000, 151: 785-95.

4. Lourenço MCS, Silva MO, Fonseca LS Multidrug-resistant tuberculosis among inmates in Rio de Janeiro, Brazil. J Microbiol 2000, 31: 17-9.

5. Brasil. Manual de Bacteriologia da Tuberculose. Rio de Janeiro: Ministério da Saúde; 2nd ed., 1994.

6. Kudoh S, Kudoh M. A simple technique for culturing tubercle bacilli.Bull WHO 1974, 52: 71-82.

7. Leite CQF, Souza CWO, Leite SRA. Identification of mycobacteria bythin layer chromatografic analysis of mycolic acids and conventional biochemical method: four years of experience. Mem Inst Oswaldo Cruz 1998, 93: 801-5.

8. Tanaka II, Anno IS, Leite SRA, Cooksey RC, Leite CQF. Comparison of amultiplex-PCR with mycolic acids analysis and conventional methods for identification of mycobacteria. Microbiol Immunol 2003, 47: 307-12.

9. Eisenach KD. PCR amplification of a repetitive DNA sequence specificfor M. tuberculosis. J Clin Dis 1990, 161: 977-81.

10. Shih JY, Hsueh PR, Lee LN, Wang HC, Yang PC, Kuo SH et al. Nontuberculous mycobacteria isolates, clinical significance and diseases pectrum. J Formos Med Assoc 1997, 96: 621-7.

11. Tartaglione T. Treatment of nontuberculous mycobacterial infections: role of clarithromycin and azithromycin. Clin Ther 1997, 19: 626-38.

12. Jonas V, Alden MJ, Curry JI, Kamisango K, Knott CA, Lankford R etal. Detection and identification of Mycobacterium tuberculosis directly from sputum sediments by amplfication of rRNA. J Clin Microbiol1993, 31: 2410-16.

13. Reichler MR, Reves R, Bur S, Thompson V, Mangura BT, Ford J et al. Evaluation of investigations conducted to detect and preventtransmission of tuberculosis. JAMA 2002, 27: 992-5.

14. Ministério da Saúde. Secretaria de Vigilância em Saúde. Divisão de Vigilância Epidemiológica. Coordenação de Doenças Endêmicas. Área Técnica de Pneumologia Sanitária. Documento de indicadores e metas para o Programa Nacional de Controle da Tuberculose,2004.

15. Secretaria Municipal de Saúde de Rio Verde, GO. Informações fornecidas referentes ao ano de 2002.

16. Aydin IO, Ulusain A. Depression, anxiety comorbidity, anddisability in tuberculosis and chronic obstructive pulmonary disease patients: applicability of GHQ-12. Gen Hosp Psychiatry 2001, 23:77-83.

17. Rabahi MF, Almeida Neto JC. Tuberculose: Risco ocupacional em profissionais de saúde. Rev Patol Trop 2001, 30: 1-8.

18. Kritski AL, Marques MJ, Rabahi MF, Vieira MA, Werneck-Barroso E,Carvalho CE et al. Transmission of tuberculosis to close contacts of patients with multidrug-resistant tuberculosis. Am J Respir Crit CareMed 1996, 153: 331-5.

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