Determination of quinolones by high performance liquid chromatography using a monolitic reversed-phase column
Vol. 64 No. 1 (2005), ORIGINAL ARTICLE
Vol. 64 No. 1 (2005)

Determination of quinolones by high performance liquid chromatography using a monolitic reversed-phase column

ORIGINAL ARTICLE
https://doi.org/10.53393/rial.2005.64.33035
Published 2005-01-03
Blanca Elena O. Markman
Instituto Adolfo Lutz, Seção de Antibióticos do Serviço de Medicamentos, Divisão de Bromatologia e Química, São Paulo, SP
Maria Regina W. Koschtschak
Instituto Adolfo Lutz, Seção de Antibióticos do Serviço de Medicamentos, Divisão de Bromatologia e Química, São Paulo, SP
Helena M. Yano
Instituto Adolfo Lutz, Seção de Antibióticos do Serviço de Medicamentos, Divisão de Bromatologia e Química, São Paulo, SP
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Keywords

quinolones
ciprofloxacin
norfloxacin
HPLC

How to Cite

1.
Markman BEO, Koschtschak MRW, Yano HM. Determination of quinolones by high performance liquid chromatography using a monolitic reversed-phase column. Rev Inst Adolfo Lutz [Internet]. 2005 Jan. 3 [cited 2024 May 19];64(1):65-9. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/33035
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Abstract

Quinolones are synthetic antibacterial agents used for treating a wide variety of infections of urinary tract, gastrointestinal tract, and skin. The aim of this study was to develop a method for performing the determination of ciprofloxacin, ciprofloxacin ethylenediamine analog (a related compound), and norfloxacin by means of high performance liquid chromatographic (HPLC) technique. The compounds were separated on a Chromolith RP18 column, with isocratic system. The mobile phase was a mixture of phosphoric acid 0.042 mol/L, pH 3.0 ± 0.1: acetonitrile (87:13), flow rate of 1.6 mL/min and UV detection at 275nm. The range of calibration curve was 20.0-125.0 µg/mL for ciprofloxacin and 20.0-100.0 µg/mL for norfloxacin. The correlation coefficient was 0.9999 for ciprofloxacin and 0.9999 for norfloxacin. The retention time for ciprofloxacin was 3.4 min, for ethilenodiamine ciprofloxacin was 2.4 min, and 2.9 min for norfloxacin. Resolution between ciprofloxacin and ciprofloxacin ethylenediamine analog was 1.1. The proposed HPLC method is rapid, simple, accurate, reproducible, and it can be applied in analyzing raw-materials, as well as in determining ciprofloxacin, ciprofloxacin ethylenediamine and norfloxacin in diverse formats as capsules, tablets, and in solution for infusion.
https://doi.org/10.53393/rial.2005.64.33035
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