Keywords
mycobacteria
Mycobacterium tuberculosis
non tuberculous mycobacteria
How to Cite
Abstract
Careful examination of occurrence of mixed mycobacterial cultures is important because most of them include at least one pathogenic agent. Among 8, 036 mycobacterial cultures samples received between 1999 and 2000 at the Mycobacteria Laboratory of Instituto Adolfo Lutz, 21 (0.26%) classified as mixed cultures were selected for being investigated. For this, the use of 7H11 plate was included for colonies isolation. The isolated colonies were then subcultured into Lowenstein-Jensen media and incubated at 37ºC. The isolates were identified by phenotypic tests and by PCR restriction enzyme analysis of hsp65 gene. The analysis of those 21 mixed cultures showed the occurrence of M. tuberculosis and non-tuberculous mycobacteria (NTM) in eight samples, three cultures with two species of NTM , and ten with only one specie of mycobacteria. The results of this study suggest that microbiology technicians should be aware of mixed cultures and apply every practical method available to separate and to identify the involved mycobacteria species.
References
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Copyright (c) 2005 Instituto Adolfo Lutz Journal
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