Abstract
Laboratory investigations of Escherichia coli (enteropathogenic coli) associated with enteric infection of children are reported, Specimens of feces were collected from the following groups of children: group I composed of 40 infants aged 1 month to 5 years whose material was collected on admission. The components of this group were admitted with acute enteritis and the material was examined by technics of isolation in order to compare these technics; group II was composed of 90 infants aged 30 days to 2 years with symptoms of severe enteric disturbances; group III was composed of 159 premature or newborn infants, all hospital delivered, with less than 30 days old. 138 of them had symtoms of an acute gastroenteritis and 21 were normal. Cultures on 74 specimens of feces collected by rectal swab from group I gave similar results to those obtained on 74 specimens of feces collected by spontaneous defecation on the same group. The investigators discovered that the use of enriched media (Gerbeaux, 1955) did not increase the number of positive cultures. Superior results were obtained by the combined use of SS and Holt-Harris-Teague media. The identification of the E. coli was based on morphology and fermentation reactions. Antigenic analysis was performed by slide agglutination followed by slow tube agglutination. The frequency of enteritis by E. coli (enteropathogenic coli) was high in all groups but higher in the newborn group (group III). The technics of agglutination, haemagglutination and microflocculation were compared for the detection of serum antibodies. The authors favor the last technic although none of the results was satisfactory. From all isolated strains E. coli O 111 B4 tests of sensivity in vitro (disk method) for the following drugs were performed: neomycin, polymyxin, sulphadiazine, chloramphenicol, oxytetracycline, chlortetracycline and streptomycin. The authors came to the conclusion that antibiograms made with germs isolated from the first and second group were similar. In the third group it is noticed an increased resistance to tetracycline and chloramphenicol. They emphasize the importance of to be acquainted with the local situation regarding the individual resistance of the patient against the commonest antibiotics used. Using a similar technic, the authors made a search for normal carriers of enteropathogenic coli and isolated from 1027 samples 30 pathogenics. Finally, they tried experimental infection on the guinea pig by introducing the germs directly into the stomach. In 16 infected animals, there were positive results in 15 cases with clinic and histopathologic picture of severe and diffuse enteritis and recovery of the germ from the feces.
References
1. ADAMSON,C. A., S. LOFGREN,C. MALMNAS- 1951 - Antibodies in mothers and newborn infants. Scand. J. clin. Lab. Invest., 3: 52-57.
2. ASSIS,A. - 1935 - Shigeloses crônicas. Arch. bras. Meâ., 25: 133-149.
3. ASSIS,A. - 1948 - Shigella guanabara, tipo sorológico destacado do grupo B. ceylonensis-dispar. O Hospital, Rio de Janeiro, 33: 505-518.
4. BRAY,J. - 1945 - Isolation of antigenically hornogeneous strains of Bact. coli neapolitanum from díarrhoea of infants. J. Path. Bact., 57: 239-247.
5. CHABBERT, Y., F. BOYER,& M. DECHAVASSINE- 1953 - Détermination de la sensíbílíté microbienne aux sulfamides par un méthode de disques séchés. Ann. Inst. Pasteur, 85: 56-63.
6. CHARTERR. E. & J. TAYLOR- 1952 - Cultural and serological reactions of strains of Bact. coli isolated from babies. J. Paih, Btici., 64: 729-734.
7. CRAIGE,J. - 1931 - Studies on the serological reactions of the flagella of B. typhosus. J. Immunol., 21: 417-511.
8. DE BEER,E. J. & M. B. SHERWOOD- 1945 - The paper-disc agar plate method for the assay of antibiotic substances. J. Bact., 50: 459-467.
9. ERICSSON,H., C. HOGMAN& K. WICKMAN- 1954 - A papel' disk method for determination of bacterial sensitivity to chemotherapeutic and antibiotic agents. Scand. J. clin, Lab. Invest., 6 (supl, 11): 23-36.
10. EWINGW. H., K. E. TANNES& H. W. TATUM- 1955 - A new serotype of Escherichia colí associated wíth infantile díarrhea, Publ. Hlth. Rep., 70: 107-114.
11. FERGUSON,W. W. & B. C. JUNE - 1952 - Experiments on Ieedíng adult volunteers with Escherichia coZi 0111 B4, a coliform organism associated with infant diarrhea. Amer. J. Hyg., 55: 155-169.
12. FRETER,R. - 1955 - The fatal enteric cholera infection in the guinea píg, achieved by inhibition of normal enteric flora. J. In], Dis., 97: 57-65.
13. FRETER,R. - 1956 - Experimental enteric Shigella and Vibrio infections in mice and guinea pigs. J. Exp. Med., 104: 411-418.
14. GERBEAUX,C. - 1955 - Milieu d'enrichessement et technique d'isolement des E. coli des gastro-entérites infantiles. Ann. Inst. Pasieur, 88: 790-793.
15. GILES C. & G. SANGSTER- 1948 - An outbreak of infantile gastro-enteritis in Aberdeen. J. Hyg., 46: 1-9.
16. HARDY, A .V. & M. M. GALTON - 1955 - The role of food processíng pIants m the dissemination of Salmonella. Amer. J. trop. Med. Hyg., 4: 725-730.
17. HARDY, A. V. & J. WATT - 1944 - Newer procedures in laboratory diagnosis and therapy ín the control of bacillary dysentery. Amer. J. Pub. Healih, 34: 503-509.
18. HORMAECHE,E., C. A. PELUFFO & P. L. ALEPPO - 1936 - Nueva contibución al estudio etioIógico de Ias "díarreas infantiles de verano". Arch. urug. Med., 9: 113-162.
19. HUNTER, C. A. & B. COLBERT - 1956 - FlocuIation tests for Brucellosis. J. Immunol. 77: 232-241.
20. KAUFFMANN, F. - 1954 - Enterobacteriaceae. Ejnar Munksgaard Publisher, Copenhague.
21. KOYA, G., N. KOSAKAI, M. KONO, M. MORI & Y. FUKASAWA - 1954 - Observations on the multípllcatíon of Escherichia coli 0-111 B4 in the intestinal tract of aduIt volunteers ín feeding experíments. The intubation study wíth MíllerAbbott's double lumen tube. Jap. J. medo Sci. Biol., 7: 197-204.
22. MAROJA, R. C. & W. D. LOWERY - 1956 - Eficácia relativa da coprocultura comparada ao swab-retal no isolamento de Shigellas. Rev. Servo Saúde públ., Rio de Janeiro, 8: 577-583.
23. MEDICALRESEARCH COUNCIL - 1953 - Antibiotic and chemotherapeutic agents ín the treatment of infantile díarrhoea and vominting. Lancet, 2: 1163-1169.
24. NETER, E., L. F. BERTRAM,D. A. ZAK, M. R. MURDOCK& C. E. ARBESMAN- 1952 Studies on hemagglutínation and hemolysís by Escherichia coli antísera. J. exp. Med., 96: 1-15.
25. NETER, E., N. J. ZALEWSKI & W. W. FERGUNSON - 1953 - Escherichia coli hemagglutinin response of adults volunteers to ingested E. coli O 55 B5. Proc. Soc. exp. Biol., N. Y., 82: 215-219.
26. NETER, E., O. WESTPHAL, O. LUDERITZ, M. R. GINO & A. E. GORZYNSKI 1955 - Demonstration of antibodies against enteropathogenic Escherichia coZi ín sera of children of various ages. Pediatrics, 16: 801-808.
27. NOVAES,J. R. C., A. E. TAUNAY & S. S. ALMEIDA- 1949 - Tipagem de Salmonelas no Laboratório de Saúde Pública. Rev. Inst. Aâolio Lute, 9: 115-122.
28. OCKLITZ, H. W. & E. F. SCHMIDT - 1955 - Vber das Vorkommen von Dyspepsie-Coli bei Erwachsenen. Helvet. paediat. acta, 10: 450-461.
29. ORSKOV, F. - 1951 - On the occurrence of E. coli belongíng to O-group 26 ín case of infantile diarrhoea and whíte scours. Acta path. microbiol. scanâ., 29: 373-378.
30. PAYNE, A. M. M. & G. T. COOR: - 1950 - A specific sorological type of Bact. coli found in infants' home in absence of epidemic díarrhoea. Brit. medo J., 2: 192-195.
31. RUGAI, E. - Informação pessoal. STEVENSON, S. S. - 1952 - Further observatíon on the occurrence of Bact. coZi D. 433 ín adult faeces. Brit. medo J., 2: 123-124.
32. TAUNAY, A. E. - 1951 - Bacteriologia das Shigeloses. Rev. Inst. Adolfo Lutz, 11: 49-102.
33. TAUNAY, A. E., J. F. PONTES, E. PRADO & E. S. PEIXOTO - 1956 - Shigeloses. Comparação de método de colheita das fezes no diagnóstico bacteriológico das enterocolites crônicas. Rev. Inst. Aaouo Lutz, 16: 37-61.
34. TAUNAY, A. E., J. C. SOARES BrcUDO, A. CORRÊA & E. S. PEIXOTO - 1956 - Estudo bacteriológico da diarréia do recém-nascido. O Hospital, Rio de Janeiro, 49: 625-634.
35. TAYLOR, J., B. W. POWELL & J. WRIGHT - 1949 - Infantile diarrhoea and vomítíng. A clinical and bacteriological investigation. Brit. medo J., 2: 117-125.
36. TROMAS, M. E. M. - 1954 - Disadvantages of the rectal swab ín díagnosís of diarrhoea. Brit. med. J., 2: 394-396.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 1958 Instituto Adolfo Lutz Journal