Keywords
quantification
direct method
chilled broiler carcasses
How to Cite
Abstract
The direct method for detecting and enumerating Campylobacter spp. in chicken meat is easy to perform, but the volumes of 100μL and 400μL, as recommended in some methodologies, are often unable to achieve the colonies counts due to the occurrence of confluent growth or microbial contaminants. This study aimed at evaluating the different volumes for rinsing chicken meats in order to minimize the interference of microbial contaminants, without compromising the sensitivity of direct methodology. Rinse volumes of 5, 10, 50, 100 and 400μL were tested using the modified charcoal cefoperazone deoxycholate agar (mCCDA) and modified Bolton agar. The presence of Campylobacter was confirmed by phenotypic methods and PCR assay. However, the strategy of using less than 100 μL of rinse volumes did not improve the isolation and enumeration of Campylobacter colonies, because it reduces the sensitivity of the assay. Thus, a possible solution to minimize the interference of microbial contaminants would be in developing new selective media or incorporate them into other existing antimicrobial drugs.References
1. Ahmed R, León-Velarde CG, Odumeru JA. Evaluation of novel agars for the enumeration of Campylobacter spp. in poultry retail samples.J Microbiol Methods.2012;88:304-10. Disponível em: [http://www.ncbi.nlm.nih.gov/pubmed/22226753].
2. Hutchison ML, Walters LD, Allen VM, Mead GC, Howell M. Measurement of Campylobacter numbers on carcasses in British poultry slaughterhouses. J Food Prot.2006;69:421–4. Disponível em: [http://www.ncbi.nlm.nih.gov/pubmed/16496586].
3. Habib I, Sampers I, Uyttendaele M, Berkvens D, Zutter LD. Performance characteristics and estimation of measurement uncertainty of three plating procedures for Campylobacter enumeration in chicken meat.Food Microbiol.2008;25:65-74. Disponível em: [http://www.ncbi.nlm.nih.gov/pubmed/17993378].
4. Medeiros VM, Bricio SML, Filgueiras ALL, Clementino MCM. Use of Bolton broth for selective enrichment and comparative analysis of its performance with direct plating methodology for isolating Campylobacter spp. from chilled chicken carcasses.Rev Inst Adolfo Lutz.2012;71:456-61. Disponível em: [http://revistas.bvs-vet.org.br/rialutz/article/view/5255/4519].
5. Franchin PR, Aidoo KE, Batista CRV. Sources of poultry meat contamination with thermophilic Campylobacter before slaughter. Braz J Microbiol.2005;36:157-62. Disponível em: [http://www.scielo.br/pdf/bjm/v36n2/arq11.pdf ].
6. Oliveira TCRM, Barbut S, Griffiths MW. A robotic DNA purification protocol and real-time PCR for the detection of Campylobacter jejuni in foods. J Food Prot.2005;68:2131-5. Disponível em: [http://www.ncbi.nlm.nih.gov/pubmed/16245718].
7. ISO. International Organization for Standardization - ISO 10272 Microbiology of Food and Animal Feeding Stuff - Horizontal Method for Detection and Enumeration of Campylobacter spp. - Part 2: Enumeration Method. ISO, Geneva, Switzerland, 2006.
8. Richardson LJ, Cox NA, Bailey JS, Berrang ME, Cox, JM, Buhr RJ, et al. Evaluation of TECRA broth, Bolton broth, and Direct Plating for recovery of Campylobacter spp. from Broiler Carcass Rinsates from Commercial Processing Plants. J Food Prot.2009;72:972-77. Disponível em: [http://www.ncbi.nlm.nih.gov/pubmed/19517723].
9. Franchin PR, Ogliari PJ, Batista CRV. Frequency of thermophilic Campylobacter in broiler chickens during in industrial processing in a Southern Brazil slaugterhouse. Br Poult Sci.2007;48:127-32. Disponível em: [http://www.ncbi.nlm.nih.gov/pubmed/17453803].
10. Habib I, Uyttendaele M, De Zutter L. Evaluation of ISO 10272:2006 standard versus alternative enrichment and plating combinations for enumeration and detection of Campylobacterin chicken meat. Food Microbiol.2011;28:1117-23. Disponível em: [http://www.ncbi.nlm.nih.gov/pubmed/21645809].
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