Keywords
ELISA-IgM
MAT
serovar Patoc
How to Cite
Abstract
The aim of this study was to evaluate the early detection of antibodies by the microscopic agglutination test (MAT) using saprophytic leptospira serovar Patoc (MAT-Patoc), in serum samples from patients with confirmed leptospirosis. The obtained results were compared with those found in ELISA-IgM. A hundred fifty-eight serum samples collected from patients in acute phase of illness were analysed by MAT-Patoc. Samples with titers ≥ 50 were considered positive. The sensitivity of MAT-Patoc and IgM-ELISA was 13.29 % and 28.48 %, respectively. In the first seven days of the disease, MAT-Patoc was positive in 12.29 % of the cases, while the IgM-ELISA was positive in 24.59 %. The MAT-Patoc was positive in sera from confirmed cases, and the infecting serogroups were Icterohaemorrhagiae, Cynopteri and Ballum. In conclusion, the use of MAT-Patoc test showed lower sensitivity for diagnosing leptospirosis when compared with ELISA-IgM, although positive reactivity was found in some samples with negative results. The MAT-Patoc should be used in combination with ELISA-IgM to improve the sensitivity of the diagnosis of leptospirosis in the first phase of the disease, with subsequent confirmation by standard MAT.
References
1. Levett PN. Leptospirosis. Clin Microbiol Rev. 2001;14(2):296-326. [DOI: 10.1128/CMR.14.2.296-326.2001].
2. Bharti AR, Nally JE, Ricaldi JN, Matthias MA, Diaz MM, Lovett MA, et al. Leptospirosis: a zoonotic disease of global importance. Lancet Infect Dis.2003;3:757-71. [DOI: 10.1016/S1473-3099(03)00830-2].
3. Faine S, Adler B, Bolin C, Perolat P. Leptospiraand leptospirosis. 2ª ed. Melbourne (Australia): MediSci; 1999.
4. Adler B, Faine S, Christopher WL, Chappel RJ. Development of an improved selective medium for isolation of leptospires from clinical material. Vet Microbiol.1986;12:377-81. [DOI: 10.1016/0378-1135(86)90087-8].
5. Wuthiekanun V, Chierakul W, Limmathurotsakul D, Smythe LD, Symonds ML, Dohnt MF et al. Optimization of culture of Leptospira from humans with leptospirosis. J Clin Microbiol.2007;45(4):1363-5. [DOI: 10.1128/JCM.02430-06].
6. Levett PN, Morey RE, Galloway RL, Turner DE, Steigerwal AG, Mayer LW. Detection of pathogenic leptospires by real-time quantitative PCR. J Med Microbiol.2005;54:45–9.
7. Ahmed A, Engelberts MF, Boer KR, Ahmed N, Hartskeerl RA. Development and validation of a real-time PCR for detection of pathogenic Leptospiraspecies in clinical materials. PLoS One.2009;4(9):e7093. [DOI: 10.1371/journal.pone.0007093].
8. Bedir O, Kilic A, Atabek E, Kuskucu AM, Turhan V, Basustaoglu AC. Simultaneous detection and differentiation of pathogenic and nonpathogenic Leptospira spp. by Multiplex Real-Time PCR (TaqMan) assay. Pol J Microbiol.2010;59(3):167-73.
9. Bourhy P, Bremont S, Zinini F, Giry C, Picardeau M. Comparison of real-time PCR assays for detection of pathogenic Leptospira spp. in blood and identification of variations in target sequences. J Clin Microbiol.2011;49(6):2154–60. [DOI: 10.1128/JCM.02452-10].
10. Blanco RM, Romero EC. Evaluation of nested polymerase chain reaction for the early detection of Leptospiraspp. DNA in serum samples from patients with leptospirosis. Diagn Microbiol Infect Dis.2014;78(4):343-6. [DOI: 10.1016/j.diagmicrobio.2013.12.009].
11. Ministério da Saúde do Brasil. Portal da Saúde. 2015. [acesso 2015 Mar 16]. Disponível em: [http://portalsaude.saude.gov.br/index.php/informacoes-tecnicas].
12. World Health Organization/International Leptospirosis Society. Human leptospirosis: guidance for diagnosis, surveillance and control. 2003. [acesso 2014 Out 10]. Disponível em: [http://whqlibdoc.who.int/hq/2003/WHO_CDS_CSR_EPH_2002.23.pdf].
13. Marta AG. Leptospirosis: Public health perspectives. Biologicals.2013;41:295-7. [DOI: 10.1016/j.biologicals.2013.06.010].
14. Smits HL, Ananyina YV, Chereshsky A, Dancel L, Lai-A-Fat RFM, Chee HD, et al. International Multicenter Evaluation of the clinical utility of a Dipstick Assay for detection of Leptospira-Specific Immunoglobulin M antibodies in human serum specimens. J Clin Microbiol.1999;37:2904–9.
15. Flannery B, Costa D, Carvalho FP, Guerreiro H, Matsunaga J, Da Silva ED, et al. Evaluation of recombinant Leptospiraantigen-based enzyme-linked immunosorbent assays for the serodiagnosis of leptospirosis. J Clin Microbiol. 2001;39(9):3303-10. [DOI: 10.1128/JCM.39.9.3303-3310.2001].
16. Bajani MD, Ashford DA, Bragg SL, Woods CW, Aye T, Spiegel RA, et al. Evaluation of four commercially available rapid serologic tests for diagnosis of leptospirosis. J Clin Microbiol. 2003;41(2):803-9. [DOI: 10.1128/JCM.41.2.803-809.2003].
17. Tahiliani P, Kumar MM, Chandu D, Kumar A, Nagaraj C, Nandi D. Gel purified LipL32: A prospective antigen for detection of leptospirosis. J Postgrad Med.2005;51:164-8.
18. McBride AJA, Pereira FA, Silva ED, Matos RB, Silva ED, Ferreira AGP, et al. Evaluation of the EIE-IgM-Leptospirose assay for the serodiagnosis of leptospirosis. Acta Trop. 2007;102(3):206–11. [DOI: 10.1016/j.actatropica.2007.05.002].
19. Tanganuchitcharnchai A, Smythe L, Dohnt M, Hartskeerl R, Vongsouvath M, Davong V et al. Evaluation of the Standard Diagnostics Leptospira IgM ELISA for diagnosis of acute leptospirosis in Lao PDR. Trans R Soc Trop Med Hyg.2012;106(9):563-6. [DOI: 10.1016/j.trstmh.2012.06.002].
20. Addamiano L, Babudiaeri B. Water strains of Leptospira in the serodiagnosis of human and animal leptospirosis. Bull World Health Organ.1968;39(6):925-34.
21. Corrêa MO, Natale V, Sadatsune T, Fleury GC. Practical value and use of Patoc I Leptospira semaranga in the diagnosis of human leptospirosis. Rev Inst Med Trop São Paulo.1970;12(4):284-7.
22. Girio RJS, Mathias LA. Use of saprophytic Leptospirastrains in the serodiagnosis of experimental leptospirosis in guinea-pigs (Cavia sp). Rev Inst Med Trop S Paulo. 988;30(2):91-4. [DOI: 10.1590/S0036-46651988000200007].
23. Ahmad SN, Shah SH, Ahmad FM. Laboratory diagnosis of leptospirosis. J Postgrad Med.2005;51:195-200.
24. Blanco RM, Takei K, Romero EC. Leptospiral glycolipoprotein as a candidate antigen for serodiagnosis of human leptospirosis. Lett Appl Microbiol.2009;49(2):267-73. [DOI: 10.1111/j.1472-765X.2009.02650.x].
25. Vijayachari P, Sugunan AP, Shriram AN. Leptospirosis: an emerging global public health problem. J Biosci.2008;33:557-69.
26. Helmerhorst HJ, Van Tol EN, Tuinman PR, de Vries PJ, Hartskeerl RA, Grobusch MP et al. Severe pulmonary manifestation of leptospirosis. Neth J Med.2012;70(5):215-21.
27. Stoddard RA, Gee JE, Wilkins PP, McCaustland K, Hoffmaster AR. Detection of pathogenic Leptospira spp. through TaqMan polymerase chain reaction targeting the LipL32 gene. Diagn Microbiol Infect Dis.2009;64(3):247–55. [DOI: 10.1016/j.diagmicrobio.2009.03.014].
28. Terpstra WJ, Ligthart GS, Schoone GJ. ELISA for the Detection of Specific IgM and IgG in Human Leptospirosis. J Gen Microbiol.1985;131:377-85.
29. Cumberland P, Everard COR, Levett PN. Assessment of the efficacy of an IgM-ELISA and Microscopic Agglutination Test (MAT) in the diagnosis of acute leptospirosis. Am J Trop Med Hyg.1999;61(5):731–4.
30. Levett PN, Branch SL. Evaluation of two Enzyme-Linked Immunosorbent Assay Methods for detection of Immunoglobulin M antibodies in acute leptospirosis. Am J Trop Med Hyg. 2002;66(6):745–8.
31. Sakata EE, Yasuda PH, Romero EC, Silva MV, Lomar AV. Sorovares de Leptospirainterrogans isolados de casos de leptospirose humana em São Paulo, Brazil. Rev Inst Med Trop S Paulo. 1992;34:217-21. [DOI: 10.1590/S0036-46651992000300006].
32. Romero EC, Bernardo CCM, Yasuda PH. Human leptospirosis: a twenty-nine-year sorological study in São Paulo, Brazil. Rev Inst Med Trop S Paulo.2003;45(5): 245-48. [DOI: 10.1590/S0036-46652003000500002 ].
33. Romero EC, Blanco RM, Galloway RL. Application of pulsed-field gel electrophoresis for the discrimination of leptospiral isolates in Brazil. Lett Appl Microbiol.2009;48:623-7. [DOI: 10.1111/j.1472-765X.2009.02580.x].
34. SehgalSC,VijayachariS, SharmaS, SugunanAP.LEPTO Dipstick: a rapid and simple method for serodiagnosis of acute leptospirosis. Trans R Soc Trop Med Hyg. 1999;93:161-4.
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