Keywords
saliva
immunoglobulin G
immunoassay
dot-ELISA
How to Cite
Abstract
The diagnosis of T. gondii infection is usually performed by serological tests, but the blood collection could be restricted in some groups as children. Antibodies are also found in other biological materials, such as saliva, whose sampling has been done by means of non-invasive procedure. Commercially available assays for performing antibody detection are standardized for analyzing serum samples. There are alternative techniques for detecting antibody in saliva, however they demand the use of equipment which is not easy to be used in field. Dot-ELISA is highly sensitivity and the results are of visual reading without any equipments, being available to be used in field studies, by means a rapid and efficient screening technique. Thus, a dot-ELISA with high sensitivity was standardized for detecting anti-T. gondii antibodies in saliva and serum by using samples from 20 adult volunteers. Sensitivity and specificity of the standardized dot-ELISA were similar in both saliva and serum samples, and precisely distinguishing the positive and negative samples, even in low antibody concentration-containing sample as saliva. Saliva showed to be as a potential biological material for detecting anti-T. gondii antibody in epidemiological studies on toxoplasmosis in children or other protected groups, where the blood collection is restricted.
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