Detecção de Leishmania (Viannia) braziliensis por PCR em amostras coletadas por raspagem de bordas de lesões de pacientes de uma área endêmica

Aparecida Helena de Souza Gomes; Izabel Madornado Armelin; Vera Lucia Pereira-Chioccola

doi:10.53393/rial.2015.v74.33497

Vol. 74 No. 4 (2015), BRIEF COMMUNICATION

Vol. 74 No. 4 (2015)

Detection of Leishmania (Viannia) braziliensis by PCR in samples collected by scraping the lesion edges from patients of an endemic area

BRIEF COMMUNICATION

https://doi.org/10.53393/rial.2015.v74.33497

Published 2016-08-26

Aparecida Helena de Souza Gomes⁺⁻
Izabel Madornado Armelin⁺⁻
Vera Lucia Pereira-Chioccola⁺⁻

Aparecida Helena de Souza Gomes

Instituto Adolfo Lutz, Centro de Laboratório Regional de Sorocaba, São Paulo, SP

Izabel Madornado Armelin

Instituto Adolfo Lutz, Centro de Laboratório Regional de Sorocaba, São Paulo, SP

Vera Lucia Pereira-Chioccola

Instituto Adolfo Lutz, Centro de Parasitologia e Micologia, Laboratório de Biologia Molecular de Parasitas, São Paulo, SP

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Keywords

American cutaneous leishmaniasis
Leishmania (Viannia) braziliensis
PCR

How to Cite

1.

Gomes AH de S, Armelin IM, Pereira-Chioccola VL. Detection of Leishmania (Viannia) braziliensis by PCR in samples collected by scraping the lesion edges from patients of an endemic area. Rev Inst Adolfo Lutz [Internet]. 2016 Aug. 26 [cited 2024 Jul. 22];74(4):437-41. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/33497

Abstract

American cutaneous leishmaniasis (ACL) is an infectious disease caused by Leishmania. Their diagnosis is performed in samples collected from the lesion biopsies, which has to be performed by physicians. For simplifying the sample collection, this study proposes a minimally invasive procedure, by scraping the lesion edges. Laboratory diagnosis by PCR was performed and compared with the microscopic examination, by analyzing 28 samples collected from patients with suspicion of ACL. Sample collected from the lesion edge with a sterile toothpick was divided into two aliquots. One aliquot was analyzed under direct microscopy, and the second by PCR, by using two primer pairs (one for Leishmania genus and other for L. (V.) braziliensis). Of 28 samples, 27 (96.43 %) showed concordant results in both methodologies (eight positive and 20 negative). The PCR methodology is an invaluable tool: (i) to determine the Leishmania species; (ii) to provide an alternative procedure of sample collection, when an authorized professional is not available in the respective health service;and (iii) to propose a minimally invasive procedure for collecting biological material.

https://doi.org/10.53393/rial.2015.v74.33497

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