Keywords
high performance liquid chromatography
wheat flour
corn flour
How to Cite
Abstract
Enrichment of foods with folic acid has turned out to be a common practice around the world. Recently, in
Brazil, the National Agency for Sanitary Surveillance (ANVISA) has brought into public notice a resolution
on mandatory enrichment of wheat and corn flour with folic acid. In the face of this, reliable analytical
methodologies for to determinate folic acid in flours have to be established and standardized. The aim of
the present study was to validate a methodology for folic acid analyses in enriched wheat and corn flour
carried out by High Performance Liquid Chromatography. Folic acid was extracted from the flours using
potassium hydroxide solution and acetonitrile. The extract was purified with trichloroacetic acid. For
chromatographic separation a gradient system composed by acid solution/acetonitrile was used as mobile
phase, and a C18 column was employed for vitamin separation and elution. Folic acid detection was made
at 290nm and its quantification was performed by external standardization. The evaluated method was
efficient being the recovery rates between 92 and 98%; the method presented good repeatibility (CV 0.60
to 0.98%), and detection and quantification limits were 1.3ng/g and 1.6ng/g, respectively.
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This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 2004 Revista do Instituto Adolfo Lutz
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