Keywords
vitamin A
fish
How to Cite
Abstract
The aim of this study was the extraction and determination of Vitamin A in fish muscle by
a simple and reliable method. The adapted methodology consisted of spectrophotometric determination of retinil palmitate in fish muscle, by using Bligh and Dyer3 method. To cleaned up the fish muscle extactact it was used a neutral alumina minicolumn and the determination of vitamin A was at 325 nm. The coeficient of variation ranged from 4 to 7% and recovery was 91%. It was higher when compared to the obtained on Carr Price’s colorimetric method, 57 and 75%. The methodology was applied to determine vitamin A in fish samples “in natura”, industrialized and heat processed (baked), maintaining their characteristics of accuracy and precision, exception when sample was canned tuna. Edible parts of bluefish (Pomatonus saltatrix) and mullet (Mugil brasiliensis) in natura resulted in 303 RE/100g (VC= 5%) and 214
RE/100g (VC=7%) respectively. In the processed products – baked bluefish 255 ER/100g (VC= 4).
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