Keywords
cell culture
cytotoxicity
Draize
How to Cite
Abstract
Although tests using animals are still demanded in the evaluation of shampoos, there is a strong evidence of a tendency for replacing them by in vitro systems. This study aims at defining
conditions for the application of a method using NCTC clone 929 cells, as an alternative. Procedures
employed to test non diluted samples of shampoos for adults resulted in irritation (in the in vivo method); the same result was not observed when samples diluted to a 25% concentration were tested. In the in vitro tests, the same shampoos presented a perfect correlation of results when not diluted (cytotoxic) and when
diluted to a 0.1% concentration (non cytotoxic). When the shampoos for children use were tested, the only case of irritation happened with one of the 4 non-diluted shampoos The absence of cytotoxicity was observed in the in vitro test, after diluting all the samples to 1% and 0.1%. It is possible thus to use the in vitro test instead of the vivo one, if preceded by a careful validation with emphasis on the concentration
of the product.
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