Mycobacteria isolation in clinical specimens using the MB/BacTTM system
pdf (Português (Brasil))

Keywords

Mycobacterium tuberculosis
diagnostic
infection TB/HIV
MB/BacTTM

How to Cite

1.
Aily DCG, Sato DN, Martins MC, Telles MA da S, Tuberculose RE de L da. Mycobacteria isolation in clinical specimens using the MB/BacTTM system. Rev Inst Adolfo Lutz [Internet]. 2003 Dec. 30 [cited 2024 May 18];62(3):233-7. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/34905

Abstract

The increasing incidence of tuberculosis and other mycobacterial diseases has made it essential for laboratories to quickly detect and identify mycobacteria. A total of 791 clinical specimens were collected from patients who were HIV positive, between March and December 2000. The MB/BacTTM system has detected 30.0% of mycobacterial isolates, while 18.8% were detected by Löwenstein-Jensen medium. The automated MB/BacTTM system was more sensitive and rapid than the traditional method for mycobacterial recovery. The MB/BacTTM system plus a DNA AccuProbe identification method could be a useful tool for the TB Control Program in rapid diagnosing TB cases among HIV positive patients.

https://doi.org/10.53393/rial.2003.62.34905
pdf (Português (Brasil))

References

1. Alcaide, F. et al. Evaluation of the BACTEC MGIT 960 and the MB/BacT systems for recovery of mycobacteria from clinical specimens an for species identification by DNA Accu Probe. J. Clin. Microbiol., 38 (1): 398-401, 2000.

2. Badak, F. Z. et al. Use of nucleic acid probes for identification of Mycobacterium tuberculosis directly from MB/BacT bottles. J. Clin. Microbiol., 37 (5): 1602-1605, 1999.

3. BRASIL, Ministério da Saúde. Fundação Nacional de Saúde. Centro de Referência Professor Hélio Fraga. Manual de Bacteriologia da Tuberculose, 2ª edição, Rio de Janeiro, 1994.

4. Brunello, F.; Favari, F.; Fontana, R. Comparison of the MB/BacT and BACTEC 460 TB systems for recovery of mycobateria from various clinical specimens. J. Clin. Microbiol., 37 (4): 1206-1209, 1999.

5. Conville, P. S. & Witebsky, F. G. Inter-bottle transfer of mycobacteria by the BACTEC 460. Diagn.Microbiol. Infect. Dis., 12: 401-405, 1989.

6. Kent, P. T. & Kubica, G. P. Public Health Mycobacteriology: a guide for the level III laboratory. Atlanta, US Department of Health and Human Services, 1985.

7. Manterola, J.M. et al. Comparison of a non radiometric system with BACTEC 12B and Culture on Egg-Based Media for Recovery of Mycobacteria from clinical specimens. Eur. J. Clin. Microbiol. Infect. Dis., 17:773-77, 1998.

8. Piersimoni et. al. Comparison of MBBacT Alert 3D System with radiometric BACTEC system and Löwenstein-Jensen Aily, D.C.G. et al. - Isolamento de micobactérias em espécimes clínicos, utilizando o sistema automatizado MB/BacTTM . Rev. Inst. Adolfo Lutz, 62(3): 233 - 237, 2003. medium for recovery and identification of mycobacteria from clinical specimens: A Multicenter study. J. Clin. Microbiol., 39:651-57, 2001.

9. Roberts G.D.; Bottger E.C.; Stockman L. Methods for the rapid identification of mycobacterial species. Clinics Lab. Med., 16(3):603-13, 1996.

10. Roggenkamp, A. et al. Comparison of MB/BacT and th BACTEC 460 TB systems for recovery of mycobacteria in a routine diagnostic laboratory. J. Clin. Microbiol., 37 (11): 3711-3712, 1999.

11. Rohner, P. et al. Evaluation of de MB/BacT system in comparison to the BACTEC 460 system and solid media for isolation of mycobacteria from clinical specimens. J. Clin. Microbiol., 35(12):3127-3131, 1997.

12. Rosemberg, J. Tuberculose Panorama Global, óbices para o seu controle. Secretaria da Saúde do Estado do Ceará – 1999.

13. São Paulo, Centro de Vigilância Epidemiológica “Prof. Alexandre Vranjac”. (Informe Técnico – Campanha Estadual de Busca de Casos de Tuberculose). Alerta TB/1998.

14. Wolinsky, E. Conventional diagnostic methods for tuberculosis. Clin. Infect. Dis., 19: 396-401, 1994.

15. World Health Organization. (Laboratory Services in Tuberculosis Control.) Part III: Culture. Geneva 1998.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright (c) 2003 Revista do Instituto Adolfo Lutz

Downloads

Download data is not yet available.