Keywords
fenotipic identification
PCR
How to Cite
Abstract
The rapidly growing mycobacteria, previously classified as Mycobacterium fortuitum complex, has recently been designated M. fortuitum, M. peregrinum, M. chelonae and M. abscessus. The identification of these mycobacteria is important for establishing the proper treatment, because they have different patterns to drugs. Among 3,441 cultures of mycobacteria received in 1999 by the Mycobacteria Laboratory of the Adolfo Lutz Institute, 13 cultures classified as M. fortuitum complex were selected to be studied. The study included isolation of colonies in order to ensure the purity of the cultures, the addition of tests with carbohydrate and citrate, which differentiate the four previously mentioned species and the use of the PCR restriction analysis of hsp65 (PRA) for the analysis of dubious results in conventional tests. In the analysis of the 13 cultures, it was observed that eight showed two types of colonies (smooth
and rough) and the remaining five showed only one type. The identification by PRA technique and by conventional tests agreed to the majority of the strains. The results of this study suggest that the identification of mycobacteria, due to its complexity, should be centralized in Reference Laboratories and implemented with specific tests for each specie in order to give a rapid diagnosis.
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