Keywords
cell proliferation
cervical squamous epithelium
immunohistochemistry
How to Cite
Abstract
To assess proliferative and apoptotic rates in squamous lesions of the uterine cervix. Material and Methods: 81 cervical biopsies were classified as cervical intraepithelial neoplasia grade 1 CIN1 = 20, CIN2 = 19, CIN3 = 23 and squamous cell carcinoma (SCC) = 19, according to WHO classification. Histological
sections were submitted to MIB-1 immunostaining by streptavidin-peroxidase amplification. In situ endlabeling of DNA strand breaks by TUNEL method was used to enhance the detection of apoptosis. All cells representative of the pertinent lesion were counted in each sample in digital photomicrographs and the results were expressed as positive cells %. The groups were compared using a one-way analysis of variance (ANOVA). Statistical significance was defined as a P< 0.05. Results: There was progressive
increase of proliferation rate (%) with the severity of lesion CIN 1 = 22.7; CIN 2 = 34.5; CIN 3 = 38.3; SCC = 52.6 p<0.0001). The same was obtained with apoptotic rate (%) (CIN 1 = 0.30; CIN 2 = 0.55; CIN 3 = 0.70; SCC = 1.19 p<0.0001), and turnover rate (%) (CIN 1 = 23.0; CIN 2 = 35.0; CIN 3 = 39.0; SCC = 53.8 p<0.0001). Surprisingly, the ratio of proliferation/apoptotic rate did not...
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