Keywords
defibrinated blood
sheep blood collection
contamination
hemoderivatives
How to Cite
Abstract
Animal-origin hemoderived products are largely employed for culture media that account for laboratory practice of interest for Public Health. In order to evaluate the quality of this product, two
systems of blood collection were tested, the traditional system that makes use of syringe, and a bag system made of atoxic polyvinyl chloride (PVC). Forty adult sheep, males and females, distributed at random in two groups of twenty animals each, were used. Blood used in this study was collected with
asepsis, in a sterile environment, incubated at 37 °C for 24 hours, and then streaked out on Mueller Hinton
blood agar plates and seeded in tubes with Brain Heart Infusion Broth (BHI). Our data showed that the PVC bags did not present any bacterial growth in the tested samples, indicating to be better than the traditional system.
References
1. Antunes G.S. Meios de Cultura. Manual de Diagnóstico Bacteriológico. 2a ed. Porto Alegre: Editora da Universidade/UFRGS; 1998. p. 242-266.
2. Colégio Brasileiro de Experimentação Animal. Princípios Éticos na Experimentação Animal. [ http://www.meusite.com.br/COBEA/etica.htm ] 03 março 2000.
3. Forney, J.E. Quality control of culture media. In: Lennette, E.H., editor. Manual of Clinical Microbiology. 4 th ed. Washington: ASM Press; 1985. p.1037 – 1050.
4. Morton, D.B et al. Removal of blood from laboratory mammals and birds. Lab. Anim., 27:1-22, 1993.
5. Moura, R.A. Meios de cultura. In: Moura, R.A., coord. Técnicas de Laboratório. 3a ed. São Paulo: Atheneu; 2002. p.169-179.
6. Nash, P.; Krenz, M.M. Culture media. In: Ballows, A., editor. Manual of Clinical Microbiology. 5 th ed. Washington: ASM Press; 1991. p. 1226-1288.
7. Russell, W.M.S; Bush, R.L. The Principles of Humane Experimentation Technique. Special Edition. Potters Bar, UK: UFAW; 1992. 238 p.
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