Keywords
apple juice
patulin
HPLC
How to Cite
Abstract
Patulin is a mycotoxin produced by several species of moulds (Penicillium, Aspergillus and Bissochlamys), but mainly by the apple-rotting fungus Penicillium expansum. Patulin is a highly toxic compound that has been shown to be mutagenic, carcinogenic and teratogenic in experiments with animals. The patulin content of apple juice is a good indicator of the quality of the apples used in the process. Thirteen brands were collected from retail markets in the city of Belo Horizonte, state of Minas Gerais, Brazil, between March and September 1999. A rapid reversed-phase liquid chromatographic determination of patulin was used. Patulin was extracted with ethyl acetate and the extract was cleaned up by partition with sodium carbonate solution. The identification and quantification of patulin was determined by C18
reversed phase column (3 µm) and a variable-wavelength UV-VIS detector set at 278 nm. Patulin and 5-hydroxymethylfurfural were completely resolved by using 0,8% tetrahydrofuran in water containing 0,02% sodium azide as the mobile phase at a flow rate of 0,5 mL/min. The detection limit was < 5 µg/L. Average recoveries greater than 73% of patulin and coefficients of variation of less than 7% were obtained. Patulin was detected in only one sample (10 µg/L). Although the incidence...
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