Enhanced sensitivity of dot-immunobinding assays for the detection of antibodies in mice by the use of mild detergent extracted Trypanosoma cruzi antigens
Vol. 50 No. 1-2 (1990), ORIGINAL ARTICLE
Vol. 50 No. 1-2 (1990)

Enhanced sensitivity of dot-immunobinding assays for the detection of antibodies in mice by the use of mild detergent extracted Trypanosoma cruzi antigens

ORIGINAL ARTICLE
https://doi.org/10.53393/rial.1990.50.35061
Published 1990-12-28
Elizabeth Natal de Gaspari
Seção de Imunologia do Instituto Adolfo Lutz, São Paulo
Ana Maria S. Stolf
Departamento de Parasitologia do Instituto de Ciências Biomédicas, Universidade de São Paulo
Ises A. Abrahamsohn
Departamento de Imunologia do Instituto de Ciências Biomédicas, Universidade de São Paulo
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Keywords

Trypanosoma cruzi
Dot-immunobinding assays
epimastigotes
trypomastigotes
non-ionic detergent

How to Cite

1.
Gaspari EN de, Stolf AMS, Abrahamsohn IA. Enhanced sensitivity of dot-immunobinding assays for the detection of antibodies in mice by the use of mild detergent extracted Trypanosoma cruzi antigens. Rev Inst Adolfo Lutz [Internet]. 1990 Dec. 28 [cited 2025 Jan. 10];50(1-2):231-3. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/35061
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Abstract

A method for a rapid and accurate demonstration of antibodies to Trypanosoma cruzi by using nitrocellulose paper strips with antigens of the parasite was developed and evaluated. We report herein additional advantages of its use for the detection of specific antibody to Trypanosoma cruz i in the sera of mice. Increased sensibility without increase in the background was achieved using parasite antigens obtained by mild non-ionic detergent extraction as compared to antigens extracted by alkaline digestion.

https://doi.org/10.53393/rial.1990.50.35061
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References

1. ARAÚJO, F.G. - A method for demonstration of antibodies to Trypanosoma cruzi by using antigen coated nitrocell~lose paper strips. Am. J. Trop. Med. Hyg., 34. 242-245, 1985.

2. USSALDO; A.M.; STOLF, A.M.S.; HOSHINO SHIMIZU, S. & CAMARGO, M.E. - Padronização da técnica de immunodot para sorologia da doença de Chagas. In: Reunião Anual sobre Pesquisa Básica em Doença de Chagas, 12°, Caxambu, 1986. Proccedings, Caxambu, Minas Gerais, 1986. p. 99.

3. PAPPAS, M.G.; HAYKOWSKI, R.; HACKMEYER, W.T. - Dot enzme-linked immunosorbent assay (Dot-EUSA): a micro technique for the rapid diagnosis of visceral Leishmaniasis. J. Immunol, Methods 64: 205-214, 1983.

4. PAPPAS, M.G.; HAYKOWSKI, R.; TANG, D.B & HOCKMEYER, W.T. - Reduced false positive reactions in the Dot-enzyme linked immunosorbent assay for human visceral leishmaniasis. Clln, Immunol, Immunopathol., 34: 392-396, 1985.

5. SIEGEL, A.- Nonnparametric statistics for the behavioural sciences. Tokyo, Kokakushas, 1985. p. 68-74.

6. SHIMIZU, S.H.; CAMARGO, M.E. & NAGASSE, T.K.- A stable polysaccharide-hemaglutination reagent for the diagnosis of acute or recent Trypanosoma cruzi infections. Rev, Inst . Med. Trop. S. Paulo, 20: 208- 12, 1978.

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Copyright (c) 1990 Revista do Instituto Adolfo Lutz

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